9129767 MWYMG4GN 1 apa 50 date desc year Barbeau 18 https://kbarbeau.scrippsprofiles.ucsd.edu/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A50%2C%22request_next%22%3A50%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22U9XZXU72%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Manck%20et%20al.%22%2C%22parsedDate%22%3A%222024-01-08%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EManck%2C%20L.%20E.%2C%20Coale%2C%20T.%20H.%2C%20Stephens%2C%20B.%20M.%2C%20Forsch%2C%20K.%20O.%2C%20Aluwihare%2C%20L.%20I.%2C%20Dupont%2C%20C.%20L.%2C%20Allen%2C%20A.%20E.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282024%29.%20Iron%20limitation%20of%20heterotrophic%20bacteria%20in%20the%20California%20Current%20System%20tracks%20relative%20availability%20of%20organic%20carbon%20and%20iron.%20%3Ci%3EThe%20ISME%20Journal%3C%5C%2Fi%3E%2C%20%3Ci%3E18%3C%5C%2Fi%3E%281%29%2C%20wrae061.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fismejo%5C%2Fwrae061%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fismejo%5C%2Fwrae061%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Iron%20limitation%20of%20heterotrophic%20bacteria%20in%20the%20California%20Current%20System%20tracks%20relative%20availability%20of%20organic%20carbon%20and%20iron%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lauren%20E%22%2C%22lastName%22%3A%22Manck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tyler%20H%22%2C%22lastName%22%3A%22Coale%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brandon%20M%22%2C%22lastName%22%3A%22Stephens%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kiefer%20O%22%2C%22lastName%22%3A%22Forsch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lihini%20I%22%2C%22lastName%22%3A%22Aluwihare%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christopher%20L%22%2C%22lastName%22%3A%22Dupont%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%20E%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katherine%20A%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%5Cn%20%20%20%20%20%20%20%20%20%20%20%20Iron%20is%20an%20essential%20nutrient%20for%20all%20microorganisms%20of%20the%20marine%20environment.%20Iron%20limitation%20of%20primary%20production%20has%20been%20well%20documented%20across%20a%20significant%20portion%20of%20the%20global%20surface%20ocean%2C%20but%20much%20less%20is%20known%20regarding%20the%20potential%20for%20iron%20limitation%20of%20the%20marine%20heterotrophic%20microbial%20community.%20In%20this%20work%2C%20we%20characterize%20the%20transcriptomic%20response%20of%20the%20heterotrophic%20bacterial%20community%20to%20iron%20additions%20in%20the%20California%20Current%20System%2C%20an%20eastern%20boundary%20upwelling%20system%2C%20to%20detect%20in%20situ%20iron%20stress%20of%20heterotrophic%20bacteria.%20Changes%20in%20gene%20expression%20in%20response%20to%20iron%20availability%20by%20heterotrophic%20bacteria%20were%20detected%20under%20conditions%20of%20high%20productivity%20when%20carbon%20limitation%20was%20relieved%20but%20when%20iron%20availability%20remained%20low.%20The%20ratio%20of%20particulate%20organic%20carbon%20to%20dissolved%20iron%20emerged%20as%20a%20biogeochemical%20proxy%20for%20iron%20limitation%20of%20heterotrophic%20bacteria%20in%20this%20system.%20Iron%20stress%20was%20characterized%20by%20high%20expression%20levels%20of%20iron%20transport%20pathways%20and%20decreased%20expression%20of%20iron-containing%20enzymes%20involved%20in%20carbon%20metabolism%2C%20where%20a%20majority%20of%20the%20heterotrophic%20bacterial%20iron%20requirement%20resides.%20Expression%20of%20iron%20stress%20biomarkers%2C%20as%20identified%20in%20the%20iron-addition%20experiments%2C%20was%20also%20detected%20insitu.%20These%20results%20suggest%20iron%20availability%20will%20impact%20the%20processing%20of%20organic%20matter%20by%20heterotrophic%20bacteria%20with%20potential%20consequences%20for%20the%20marine%20biological%20carbon%20pump.%22%2C%22date%22%3A%222024-01-08%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1093%5C%2Fismejo%5C%2Fwrae061%22%2C%22ISSN%22%3A%221751-7362%2C%201751-7370%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Facademic.oup.com%5C%2Fismej%5C%2Farticle%5C%2Fdoi%5C%2F10.1093%5C%2Fismejo%5C%2Fwrae061%5C%2F7646369%22%2C%22collections%22%3A%5B%22QIYZ9CQ7%22%2C%22MWYMG4GN%22%2C%2286H9SNJB%22%5D%2C%22dateModified%22%3A%222024-08-23T23%3A26%3A57Z%22%7D%7D%2C%7B%22key%22%3A%226XTW8WSH%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Lampe%20et%20al.%22%2C%22parsedDate%22%3A%222023-11-08%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ELampe%2C%20R.%20H.%2C%20Coale%2C%20T.%20H.%2C%20Forsch%2C%20K.%20O.%2C%20Jabre%2C%20L.%20J.%2C%20Kekuewa%2C%20S.%2C%20Bertrand%2C%20E.%20M.%2C%20Hor%26%23xE1%3Bk%2C%20A.%2C%20Oborn%26%23xED%3Bk%2C%20M.%2C%20Rabines%2C%20A.%20J.%2C%20Rowland%2C%20E.%2C%20Zheng%2C%20H.%2C%20Andersson%2C%20A.%20J.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20%26amp%3B%20Allen%2C%20A.%20E.%20%282023%29.%20Short-term%20acidification%20promotes%20diverse%20iron%20acquisition%20and%20conservation%20mechanisms%20in%20upwelling-associated%20phytoplankton.%20%3Ci%3ENature%20Communications%3C%5C%2Fi%3E%2C%20%3Ci%3E14%3C%5C%2Fi%3E%281%29%2C%207215.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-023-42949-1%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-023-42949-1%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Short-term%20acidification%20promotes%20diverse%20iron%20acquisition%20and%20conservation%20mechanisms%20in%20upwelling-associated%20phytoplankton%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robert%20H.%22%2C%22lastName%22%3A%22Lampe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tyler%20H.%22%2C%22lastName%22%3A%22Coale%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kiefer%20O.%22%2C%22lastName%22%3A%22Forsch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Loay%20J.%22%2C%22lastName%22%3A%22Jabre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Samuel%22%2C%22lastName%22%3A%22Kekuewa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Erin%20M.%22%2C%22lastName%22%3A%22Bertrand%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ale%5Cu0161%22%2C%22lastName%22%3A%22Hor%5Cu00e1k%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Miroslav%22%2C%22lastName%22%3A%22Oborn%5Cu00edk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ariel%20J.%22%2C%22lastName%22%3A%22Rabines%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elden%22%2C%22lastName%22%3A%22Rowland%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hong%22%2C%22lastName%22%3A%22Zheng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andreas%20J.%22%2C%22lastName%22%3A%22Andersson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katherine%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Coastal%20upwelling%20regions%20are%20among%20the%20most%20productive%20marine%20ecosystems%20but%20may%20be%20threatened%20by%20amplified%20ocean%20acidification.%20Increased%20acidification%20is%20hypothesized%20to%20reduce%20iron%20bioavailability%20for%20phytoplankton%20thereby%20expanding%20iron%20limitation%20and%20impacting%20primary%20production.%20Here%20we%20show%20from%20community%20to%20molecular%20levels%20that%20phytoplankton%20in%20an%20upwelling%20region%20respond%20to%20short-term%20acidification%20exposure%20with%20iron%20uptake%20pathways%20and%20strategies%20that%20reduce%20cellular%20iron%20demand.%20A%20combined%20physiological%20and%20multi-omics%20approach%20was%20applied%20to%20trace%20metal%20clean%20incubations%20that%20introduced%201200%20ppm%20CO%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%202%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20for%20up%20to%20four%20days%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Although%20variable%2C%20molecular-level%20responses%20indicate%20a%20prioritization%20of%20iron%20uptake%20pathways%20that%20are%20less%20hindered%20by%20acidification%20and%20reductions%20in%20iron%20utilization.%20Growth%2C%20nutrient%20uptake%2C%20and%20community%20compositions%20remained%20largely%20unaffected%20suggesting%20that%20these%20mechanisms%20may%20confer%20short-term%20resistance%20to%20acidification%3B%20however%2C%20we%20speculate%20that%20cellular%20iron%20demand%20is%20only%20temporarily%20satisfied%2C%20and%20longer-term%20acidification%20exposure%20without%20increased%20iron%20inputs%20may%20result%20in%20increased%20iron%20stress.%22%2C%22date%22%3A%222023-11-08%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41467-023-42949-1%22%2C%22ISSN%22%3A%222041-1723%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41467-023-42949-1%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%2C%2286H9SNJB%22%2C%22R2U424Z5%22%5D%2C%22dateModified%22%3A%222023-12-20T00%3A43%3A41Z%22%7D%7D%2C%7B%22key%22%3A%227I4BUDRM%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Forsch%20et%20al.%22%2C%22parsedDate%22%3A%222023%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EForsch%2C%20K.%20O.%2C%20Fulton%2C%20K.%20C.%2C%20Weiss%2C%20M.%20M.%2C%20Krause%2C%20J.%20W.%2C%20Stukel%2C%20M.%20R.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282023%29.%20Iron%20Limitation%20and%20Biogeochemical%20Effects%20in%20Southern%20California%20Current%20Coastal%20Upwelling%20Filaments.%20%3Ci%3EJournal%20of%20Geophysical%20Research%3A%20Oceans%3C%5C%2Fi%3E%2C%20%3Ci%3E128%3C%5C%2Fi%3E%2811%29%2C%20e2023JC019961.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1029%5C%2F2023JC019961%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1029%5C%2F2023JC019961%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Iron%20Limitation%20and%20Biogeochemical%20Effects%20in%20Southern%20California%20Current%20Coastal%20Upwelling%20Filaments%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20O.%22%2C%22lastName%22%3A%22Forsch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20C.%22%2C%22lastName%22%3A%22Fulton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20M.%22%2C%22lastName%22%3A%22Weiss%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20W.%22%2C%22lastName%22%3A%22Krause%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20R.%22%2C%22lastName%22%3A%22Stukel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%5Cn%20%20%20%20%20%20%20%20%20%20%20%20In%20the%20spring%20and%20summer%2C%20high%20rates%20of%20primary%20production%20occur%20in%20the%20California%20Current%20Ecosystem%20%28CCE%29%20when%20nutrients%20are%20supplied%20to%20the%20euphotic%20zone.%20During%20periods%20of%20intense%20coastal%20upwelling%2C%20a%20flux%20of%20the%20micronutrient%20iron%20comes%20from%20nearshore%20sedimentary%20sources.%20In%20this%20upwelling%20region%2C%20mesoscale%20filament%20features%20distribute%20iron%20laterally%2C%20leading%20to%20distinct%20iron%5Cu2010influenced%20ecological%20zones.%20This%20study%20is%20the%20first%20to%20focus%20on%20the%20biogeochemical%20links%20between%20iron%2C%20the%20macronutrients%2C%20and%20particulates%20in%20coastal%20upwelling%20filaments.%20Broad%20spatial%20patterns%20of%20iron%20and%20biogenic%20silica%20concentrations%2C%20and%20proxies%20of%20iron%5Cu2010stress%20of%20diatoms%2C%20support%20results%20from%20microcosm%20amendment%20studies%20conducted%20during%20CCE%20Long%20Term%20Ecological%20Research%20process%20cruises%20in%20the%20summers%20of%202017%20and%202019.%20We%20found%20that%20the%20benthic%20boundary%20layer%20and%20shoreward%20filament%20endmember%20supply%20dissolved%20and%20total%20dissolvable%20iron%20to%20this%20feature%2C%20but%20rapid%20assimilation%20and%20sinking%20by%20biogenic%20particles%20%28e.g.%2C%20diatoms%29%20depletes%20the%20surface%20concentrations.%20Subsequently%2C%20diatom%20blooms%20which%20form%20in%20recently%20upwelled%20water%20masses%20become%20iron%20limited%20over%20time%2C%20thereby%20affecting%20the%20ratios%20of%20surface%20macronutrient%20reservoirs%20and%20biogeochemical%20advective%20fluxes.%20The%20development%20of%20Fe%5Cu2010limitation%20during%20lateral%20advection%20may%20lead%20to%20efficient%20carbon%20export%20downstream%20and%20offshore%20of%20the%20region%20with%20the%20highest%20phytoplankton%20growth%20rates%20and%20productivity.%5Cn%20%20%20%20%20%20%20%20%20%20%2C%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20Plain%20Language%20Summary%5Cn%20%20%20%20%20%20%20%20%20%20%20%20Along%20the%20western%20continental%20margin%20of%20the%20United%20States%2C%20seasonal%20upwelling%20filaments%20appear%20as%20fast%5Cu2010flowing%20waters%20with%20high%20concentrations%20of%20nutrients%20%28e.g.%2C%20nitrate%2C%20silicic%20acid%2C%20iron%29%20and%20phytoplankton.%20Diatoms%20dominate%20the%20phytoplankton%20communities%20in%20filaments%2C%20and%20they%20accumulate%20particulate%20matter%20as%20organic%20carbon%20and%20dense%20biogenic%20silica.%20During%20transport%20offshore%2C%20diatoms%20quickly%20deplete%20the%20surface%20of%20the%20critical%20micronutrient%20iron%20%28Fe%29%20and%20become%20Fe%5Cu2010limited.%20Using%20shipboard%20incubation%20experiments%2C%20we%20show%20that%20Fe%5Cu2010stressed%20diatom%20communities%20become%20more%20heavily%20silicified%2C%20and%20thus%20denser.%20Iron%5Cu2010stress%20indicators%20consistently%20correspond%20with%20these%20phytoplankton%20community%20biochemical%20reconfigurations%20in%20two%20separate%20studies%20of%20filaments%2C%20thereby%20demonstrating%20a%20mechanism%20for%20enhanced%20diatom%20community%20export%20out%20of%20the%20surface%20ocean%20in%20later%20stages%20of%20filaments.%5Cn%20%20%20%20%20%20%20%20%20%20%2C%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20Key%20Points%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Upwelling%20filaments%20become%20Fe%5Cu2010limited%20features%20in%20the%20ocean%2C%20as%20sinking%20diatoms%20deplete%20surface%20dFe%20concentrations%20and%20are%20advected%20offshore%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Iron%5Cu2010limitation%20of%20diatom%20blooms%20is%20evidenced%20by%20in%20situ%20geochemical%20proxies%20%28e.g.%2C%20negative%20Siex%29%20and%20deckboard%20Fe%20amendment%20experiments%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Greater%20biogenic%20silica%5Cu2010to%5Cu2010particulate%20organic%20carbon%20ratios%20among%20diatom%20communities%20make%20filaments%20hotspots%20of%20particulate%20matter%20export%22%2C%22date%22%3A%2211%5C%2F2023%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1029%5C%2F2023JC019961%22%2C%22ISSN%22%3A%222169-9275%2C%202169-9291%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fagupubs.onlinelibrary.wiley.com%5C%2Fdoi%5C%2F10.1029%5C%2F2023JC019961%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222023-12-01T18%3A43%3A56Z%22%7D%7D%2C%7B%22key%22%3A%22I95UYGN6%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Forsch%20et%20al.%22%2C%22parsedDate%22%3A%222021-12%22%2C%22numChildren%22%3A4%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EForsch%2C%20K.%20O.%2C%20Hahn-Woernle%2C%20L.%2C%20Sherrell%2C%20R.%20M.%2C%20Roccanova%2C%20V.%20J.%2C%20Bu%2C%20K.%20X.%2C%20Burdige%2C%20D.%2C%20Vernet%2C%20M.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282021%29.%20Seasonal%20dispersal%20of%20fjord%20meltwaters%20as%20an%20important%20source%20of%20iron%20and%20manganese%20to%20coastal%20Antarctic%20phytoplankton.%20%3Ci%3EBiogeosciences%3C%5C%2Fi%3E%2C%20%3Ci%3E18%3C%5C%2Fi%3E%2823%29%2C%206349%26%23x2013%3B6375.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.5194%5C%2Fbg-18-6349-2021%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.5194%5C%2Fbg-18-6349-2021%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Seasonal%20dispersal%20of%20fjord%20meltwaters%20as%20an%20important%20source%20of%20iron%20and%20manganese%20to%20coastal%20Antarctic%20phytoplankton%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20O.%22%2C%22lastName%22%3A%22Forsch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%22%2C%22lastName%22%3A%22Hahn-Woernle%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Sherrell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V.%20J.%22%2C%22lastName%22%3A%22Roccanova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20X.%22%2C%22lastName%22%3A%22Bu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%22%2C%22lastName%22%3A%22Burdige%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Vernet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22Glacial%20meltwater%20from%20the%20western%20Antarctic%20Ice%20Sheet%20is%20hypothesized%20to%20be%20an%20important%20source%20of%20cryospheric%20iron%2C%20fertilizing%20the%20Southern%20Ocean%2C%20yet%20its%20trace-metal%20composition%20and%20factors%20that%20control%20its%20dispersal%20remain%20poorly%20constrained.%20Here%20we%20characterize%20meltwater%20iron%20sources%20in%20a%20heavily%20glaciated%20western%20Antarctic%20Peninsula%20%28WAP%29%20fjord.%20Using%20dissolved%20and%20particulate%20ratios%20of%20manganese%20to%20iron%20in%20meltwaters%2C%20porewaters%2C%20and%20seawater%2C%20we%20show%20that%20surface%20glacial%20melt%20and%20subglacial%20plumes%20contribute%20to%20the%20seasonal%20cycle%20of%20iron%20and%20manganese%20within%20a%20fjord%20still%20relatively%20unaffected%20by%20climate-change-induced%20glacial%20retreat.%20Organic%20ligands%20derived%20from%20the%20phytoplankton%20bloom%20and%20the%20glaciers%20bind%20dissolved%20iron%20and%20facilitate%20the%20solubilization%20of%20particulate%20iron%20downstream.%20Using%20a%20numerical%20model%2C%20we%20show%20that%20buoyant%20plumes%20generated%20by%20outflow%20from%20the%20subglacial%20hydrologic%20system%2C%20enriched%20in%20labile%20particulate%20trace%20metals%20derived%20from%20a%20chemically%20modified%20crustal%20source%2C%20can%20supply%20iron%20to%20the%20fjord%20euphotic%20zone%20through%20vertical%20mixing.%20We%20also%20show%20that%20prolonged%20katabatic%20wind%20events%20enhance%20export%20of%20meltwater%20out%20of%20the%20fjord.%20Thus%2C%20we%20identify%20an%20important%20atmosphere-ice-ocean%20coupling%20intimately%20tied%20to%20coastal%20iron%20biogeochemistry%20and%20primary%20productivity%20along%20the%20WAP.%22%2C%22date%22%3A%222021%5C%2F12%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.5194%5C%2Fbg-18-6349-2021%22%2C%22ISSN%22%3A%221726-4170%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%2C%22IG98B7FW%22%5D%2C%22dateModified%22%3A%222022-07-27T16%3A34%3A29Z%22%7D%7D%2C%7B%22key%22%3A%22HZ9CGCBT%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Moore%20et%20al.%22%2C%22parsedDate%22%3A%222021-11%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMoore%2C%20L.%20E.%2C%20Heller%2C%20M.%20I.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20Moffett%2C%20J.%20W.%2C%20%26amp%3B%20Bundy%2C%20R.%20M.%20%282021%29.%20Organic%20complexation%20of%20iron%20by%20strong%20ligands%20and%20siderophores%20in%20the%20eastern%20tropical%20North%20Pacific%20oxygen%20deficient%20zone.%20%3Ci%3EMarine%20Chemistry%3C%5C%2Fi%3E%2C%20%3Ci%3E236%3C%5C%2Fi%3E%2C%2016.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2021.104021%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2021.104021%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Organic%20complexation%20of%20iron%20by%20strong%20ligands%20and%20siderophores%20in%20the%20eastern%20tropical%20North%20Pacific%20oxygen%20deficient%20zone%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%20E.%22%2C%22lastName%22%3A%22Moore%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20I.%22%2C%22lastName%22%3A%22Heller%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20W.%22%2C%22lastName%22%3A%22Moffett%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%5D%2C%22abstractNote%22%3A%22Continental%20margins%20are%20an%20important%20external%20source%20of%20dissolved%20iron%20to%20the%20marine%20environment.%20However%2C%20the%20mechanisms%20responsible%20for%20the%20offshore%20transport%20of%20dissolved%20iron%20is%20impacted%20by%20the%20resulting%20iron%20speciation.%20We%20characterized%20the%20iron%20speciation%20in%20the%20Eastern%20Tropical%20North%20Pacific%20%28ETNP%29%20oxygen%20deficient%20zone%20%28ODZ%29%2C%20including%20dissolved%20iron%2C%20organic%20iron-binding%20ligands%2C%20and%20reduced%20iron.%20Organic%20iron-binding%20ligands%20were%20measured%20using%20both%20competitive%20ligand%20exchange%20adsorptive%20cathodic%20stripping%20voltammetry%20%28CLE-ACSV%29%20and%20liquid%20chromatography%20electrospray%20ionization%20mass%20spectrometry%20%28LC-ESI-MS%29%20in%20order%20to%20explore%20the%20impact%20of%20organic%20ligands%20on%20dissolved%20iron%20%28dFe%28T%29%20and%20iron%28II%29%20biogeochemistry%20in%20the%20region.%20Organic%20ligands%20were%20present%20in%20high%20concentrations%20%281.06-5.30%20nmol%20L-1%29%20and%20exceeded%20dissolved%20iron%20concentrations%20%280.36-4.52%20nmol%20L-1%29%20at%20all%20locations.%20Iron-binding%20strengths%20%28logK%28FeL%2Cfe%27%29%28cond%29%29%20ranged%2011.22%20to%2012.75%20and%20were%20elevated%20in%20the%20ODZ%20layer%20relative%20to%20the%20oxygenated%20water%20column.%20LC-ESI-MS%20revealed%20the%20presence%20of%20siderophores%2C%20or%20bacteriallyproduced%20organic%20ligands%20with%20high%20Fe-affinity%2C%20in%20all%20samples%20analyzed%2C%20suggesting%20these%20compounds%20may%20be%20produced%20by%20microbes%20in%20the%20ODZ%20despite%20high%20ambient%20dFe%28T%29%20concentrations.%20This%20study%20is%20the%20first%20to%20characterize%20siderophores%20in%20an%20ODZ%20environment%20to%20date%2C%20and%20the%20three%20siderophores%20found%20%28amphibactin%20B%2C%20synechobactin%20c9%2C%20synechobactin%20c10%29%20could%20contribute%20to%20the%20observed%20elevated%20logK%28FeL%2Cfe%27%29%28cond%29%20of%20ligands%20in%20the%20ODZ.%20Comparative%20analysis%20of%20organic%20ligand%20logK%28FeL%2Cfe%27%29%28cond%29%20values%20in%20other%20low%20oxygen%20environments%20suggests%20that%20strong%20ligands%2C%20including%20siderophores%2C%20could%20be%20present%20in%20other%20low%20oxygen%20regions.%20In%20a%20simple%20model%20of%20the%20shelf-to-offshore%20iron%20transport%20mechanism%2C%20strong%20organic%20iron-binding%20ligands%20had%20a%20large%20impact%20on%20the%20longevity%20and%20transport%20of%20iron%20in%20the%20ODZ.%20These%20results%20suggest%20that%20organic%20ligand%20composition%20can%20have%20an%20impact%20on%20iron%20distributions%20in%20the%20ETNP%20ODZ%20and%20regulate%20the%20offshore%20transport%20of%20iron%20to%20the%20open%20ocean.%22%2C%22date%22%3A%222021%5C%2F11%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.marchem.2021.104021%22%2C%22ISSN%22%3A%220304-4203%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A16Z%22%7D%7D%2C%7B%22key%22%3A%224FSJZRPV%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Manck%20et%20al.%22%2C%22parsedDate%22%3A%222021-08%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EManck%2C%20L.%20E.%2C%20Park%2C%20J.%2C%20Tully%2C%20B.%20J.%2C%20Poire%2C%20A.%20M.%2C%20Bundy%2C%20R.%20M.%2C%20Dupont%2C%20C.%20L.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282021%29.%20Petrobactin%2C%20a%20siderophore%20produced%20by%20Alteromonas%2C%20mediates%20community%20iron%20acquisition%20in%20the%20global%20ocean.%20%3Ci%3EIsme%20Journal%3C%5C%2Fi%3E%2C%2012.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41396-021-01065-y%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41396-021-01065-y%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Petrobactin%2C%20a%20siderophore%20produced%20by%20Alteromonas%2C%20mediates%20community%20iron%20acquisition%20in%20the%20global%20ocean%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%20E.%22%2C%22lastName%22%3A%22Manck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Park%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20J.%22%2C%22lastName%22%3A%22Tully%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20M.%22%2C%22lastName%22%3A%22Poire%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20L.%22%2C%22lastName%22%3A%22Dupont%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22It%20is%20now%20widely%20accepted%20that%20siderophores%20play%20a%20role%20in%20marine%20iron%20biogeochemical%20cycling.%20However%2C%20the%20mechanisms%20by%20which%20siderophores%20affect%20the%20availability%20of%20iron%20from%20specific%20sources%20and%20the%20resulting%20significance%20of%20these%20processes%20on%20iron%20biogeochemical%20cycling%20as%20a%20whole%20have%20remained%20largely%20untested.%20In%20this%20study%2C%20we%20develop%20a%20model%20system%20for%20testing%20the%20effects%20of%20siderophore%20production%20on%20iron%20bioavailability%20using%20the%20marine%20copiotroph%20Alteromonas%20macleodii%20ATCC%2027126.%20Through%20the%20generation%20of%20the%20knockout%20cell%20line%20Delta%20asbB%3A%3Akm%28R%29%2C%20which%20lacks%20siderophore%20biosynthetic%20capabilities%2C%20we%20demonstrate%20that%20the%20production%20of%20the%20siderophore%20petrobactin%20enables%20the%20acquisition%20of%20iron%20from%20mineral%20sources%20and%20weaker%20iron-ligand%20complexes.%20Notably%2C%20the%20utilization%20of%20lithogenic%20iron%2C%20such%20as%20that%20from%20atmospheric%20dust%2C%20indicates%20a%20significant%20role%20for%20siderophores%20in%20the%20incorporation%20of%20new%20iron%20into%20marine%20systems.%20We%20have%20also%20detected%20petrobactin%2C%20a%20photoreactive%20siderophore%2C%20directly%20from%20seawater%20in%20the%20mid-latitudes%20of%20the%20North%20Pacific%20and%20have%20identified%20the%20biosynthetic%20pathway%20for%20petrobactin%20in%20bacterial%20metagenome-assembled%20genomes%20widely%20distributed%20across%20the%20global%20ocean.%20Together%2C%20these%20results%20improve%20our%20mechanistic%20understanding%20of%20the%20role%20of%20siderophore%20production%20in%20iron%20biogeochemical%20cycling%20in%20the%20marine%20environment%20wherein%20iron%20speciation%2C%20bioavailability%2C%20and%20residence%20time%20can%20be%20directly%20influenced%20by%20microbial%20activities.%22%2C%22date%22%3A%222021%5C%2F08%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41396-021-01065-y%22%2C%22ISSN%22%3A%221751-7362%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A16Z%22%7D%7D%2C%7B%22key%22%3A%22DUPUIN7J%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Ruacho%20et%20al.%22%2C%22parsedDate%22%3A%222020-09%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ERuacho%2C%20A.%2C%20Bundy%2C%20R.%20M.%2C%20Till%2C%20C.%20P.%2C%20Roshan%2C%20S.%2C%20Wu%2C%20J.%20F.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282020%29.%20Organic%20dissolved%20copper%20speciation%20across%20the%20US%20GEOTRACES%20equatorial%20Pacific%20zonal%20transect%20GP16.%20%3Ci%3EMarine%20Chemistry%3C%5C%2Fi%3E%2C%20%3Ci%3E225%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2020.103841%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2020.103841%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Organic%20dissolved%20copper%20speciation%20across%20the%20US%20GEOTRACES%20equatorial%20Pacific%20zonal%20transect%20GP16%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Ruacho%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20P.%22%2C%22lastName%22%3A%22Till%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Roshan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20F.%22%2C%22lastName%22%3A%22Wu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22Samples%20for%20organic%20copper%20%28Cu%29-binding%20ligand%20characterization%20were%20collected%20along%20the%202013%20U.S.%20GEOTRACES%20Pacific%20%28GP16%29%20cruise%20transect%20from%20Peru%20to%20Tahiti.%20Full%20depth%20profiles%20of%20Cu%20speciation%20were%20collected%20across%20a%20dynamic%20range%20in%20oceanographic%20conditions%20including%20a%20highly%20productive%20coastal%20region%2C%20an%20oxygen%20deficient%20zone%2C%20a%20high%20nutrient%20low%20chlorophyll%20%28HNLC%29%20region%2C%20an%20oligotrophic%20region%20and%20a%20hydrothermal%20vent%20plume.%20Surface%20waters%20from%20Peru%20to%20Tahiti%20exhibited%20elevated%20dissolved%20Cu%20and%20ligand%20concentrations%20near%20Peru%20and%20then%20decreased%20in%20concentration%20%28%20%3C%201%20nM%29%20offshore%20toward%20the%20oligotrophic%20waters.%20There%20was%20also%20an%20apparent%20shelf%20sediment%20source%20of%20strong%20Cu-binding%20ligands%20near%20the%20coast%20of%20Peru.%20Throughout%20most%20of%20the%20transect%20dissolved%20Cu%20and%20ligand%20concentrations%20were%20lower%20in%20the%20upper%20waters%20and%20increased%20with%20depth%2C%20with%20the%20highest%20concentrations%20near%20the%20ocean%20bottom.%20The%20hydrothermal%20vent%20sampled%20during%20the%20cruise%20did%20not%20seem%20to%20be%20a%20source%20for%20dissolved%20Cu%20but%20there%20was%20a%20slight%20elevation%20of%20Cu-binding%20ligands%20at%20the%20vent%20site.%20Similar%20vertical%20patterns%20in%20Cu-binding%20ligands%20were%20seen%20in%20both%20the%20GP16%20dataset%20and%20the%20North%20Atlantic%20GEOTRACES%20%28GA03%29%20cruise%2C%20with%20notable%20differences%20in%20deep%20waters%20of%20the%20Pacific.%20The%20older%20water%20masses%20of%20the%20Pacific%20were%20highlighted%20by%20higher%20concentrations%20of%20dissolved%20Cu%2C%20Cu-binding%20ligands%2C%20and%20the%20free%20Cu%20ion%20%28Cu2%2B%29%20relative%20to%20the%20deep%20Atlantic.%20Excess%20Cu%20ligands%20in%20both%20GP16%20and%20GA03%20point%20to%20a%20possible%20fraction%20of%20Cu%20accumulating%20in%20the%20deep%20Pacific%20that%20is%20inert%20to%20ligand%20exchange%2C%20suggesting%20older%20waters%20might%20contain%20a%20high%20fraction%20of%20unreactive%20Cu.%22%2C%22date%22%3A%222020%5C%2F09%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.marchem.2020.103841%22%2C%22ISSN%22%3A%220304-4203%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A16Z%22%7D%7D%2C%7B%22key%22%3A%22MJ9BJKHV%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Pan%20et%20al.%22%2C%22parsedDate%22%3A%222020-04%22%2C%22numChildren%22%3A4%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPan%2C%20B.%20J.%2C%20Vernet%2C%20M.%2C%20Manck%2C%20L.%2C%20Forsch%2C%20K.%2C%20Ekern%2C%20L.%2C%20Mascioni%2C%20M.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20Almandoz%2C%20G.%20O.%2C%20%26amp%3B%20Orona%2C%20A.%20J.%20%282020%29.%20Environmental%20drivers%20of%20phytoplankton%20taxonomic%20composition%20in%20an%20Antarctic%20fjord.%20%3Ci%3EProgress%20in%20Oceanography%3C%5C%2Fi%3E%2C%20%3Ci%3E183%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.pocean.2020.102295%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.pocean.2020.102295%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Environmental%20drivers%20of%20phytoplankton%20taxonomic%20composition%20in%20an%20Antarctic%20fjord%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20J.%22%2C%22lastName%22%3A%22Pan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Vernet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%22%2C%22lastName%22%3A%22Manck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Forsch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%22%2C%22lastName%22%3A%22Ekern%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Mascioni%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22G.%20O.%22%2C%22lastName%22%3A%22Almandoz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20J.%22%2C%22lastName%22%3A%22Orona%22%7D%5D%2C%22abstractNote%22%3A%22The%20impact%20of%20ice-ocean%20interaction%20on%20the%20Southern%20Ocean%20is%20expected%20to%20intensify%20in%20the%20future.%20However%2C%20its%20influence%20on%20phytoplankton%20community%20composition%20remains%20an%20open%20question.%20The%20Antarctic%20Peninsula%20fjords%20offer%20an%20ideal%20system%20to%20understand%20the%20effect%20of%20ice-ocean%20forcing%20on%20phytoplankton%20community%2C%20providing%20an%20extreme%20in%20the%20spatial%20gradient%20from%20the%20glacio-marine%20boundary%20to%20the%20Western%20Antarctic%20Peninsula%20%28WAP%29%20continental%20shelf.%20During%20two%20cruises%20conducted%20in%20December%202015%20and%20April%202016%20in%20Andvord%20Bay%2C%20we%20found%20that%20glacial%20meltwater%20input%20altered%20surface%20salinity%2C%20promoting%20shallow%20mixed%20layers%2C%20and%20enriched%20surface%20waters%20in%20dissolved%20iron%20and%20nitrate.%20The%20three%20major%20groups%20of%20phytoplankton%20fueled%20by%20glacial%20input%20were%3A%20cryptophytes%2C%20diatoms%2C%20and%20a%20group%20of%20unidentified%20small%20flagellates.%20Prasinophytes%20and%20dinoflagellates%20were%20also%20present%2C%20in%20lower%20concentrations.%20In%20December%2C%20cryptophytes%20dominated%20the%20phytoplankton%20community%20and%20were%20correlated%20with%20relatively%20warmer%20temperatures%20in%20the%20surface%20layer%3B%20in%20addition%2C%20contrary%20to%20our%20hypothesis%2C%20no%20diatom%20bloom%20was%20observed%20in%20the%20fjord%20in%20spite%20of%20dissolved%20iron%20concentration%20%3E%201%20nM.%20By%20April%2C%20after%20the%20growth%20season%2C%20the%20overall%20phytoplankton%20abundance%20had%20decreased%20by%20an%20order%20of%20magnitude.%20Phytoplankton%2C%20in%20particular%20diatoms%2C%20were%20then%20limited%20by%20daytime%20length%20despite%20abundant%20macro-nutrient%20and%20iron%20concentrations.%20Mixed%20flagellates%20emerged%20as%20the%20dominant%20group%20during%20April%20due%20to%20the%20decline%20of%20other%20major%20taxa.%20Deep-learning%20algorithms%20for%20predicting%20the%20abundance%20of%20each%20major%20phytoplankton%20group%20captured%20the%20effects%20of%20these%20environmental%20factors%20on%20the%20phytoplankton%20community.%20Our%20results%20show%20that%20the%20fjord%20has%20relatively%20high%20phytoplankton%20biomass%20combined%20with%20high%20macro-%20and%20trace%20nutrient%20concentrations%20when%20compared%20to%20the%20broader%20WAP%20region.%20Based%20on%20this%20study%2C%20we%20confirm%20that%20flagellates%20can%20be%20the%20dominant%20taxon%20in%20Antarctic%20nearshore%20waters%20and%20we%20propose%20that%20iron%20concentration%20alone%20is%20insufficient%20to%20predict%20diatom%20growth.%20Furthermore%2C%20marine%20terminating%20glaciers%20in%20the%20WAP%20can%20enrich%20surface%20waters%20with%20nitrate%20even%20if%20the%20main%20fjord%20circulation%20is%20not%20driven%20by%20glacier%20meltwater%20discharge.%22%2C%22date%22%3A%222020%5C%2F04%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.pocean.2020.102295%22%2C%22ISSN%22%3A%220079-6611%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%2C%22IG98B7FW%22%5D%2C%22dateModified%22%3A%222022-08-15T17%3A45%3A34Z%22%7D%7D%2C%7B%22key%22%3A%22DRC2I27N%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Manck%20et%20al.%22%2C%22parsedDate%22%3A%222020-03%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EManck%2C%20L.%20E.%2C%20Espinoza%2C%20J.%20L.%2C%20Dupont%2C%20C.%20L.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282020%29.%20Transcriptomic%20study%20of%20substrate-specific%20transport%20mechanisms%20for%20iron%20and%20carbon%20in%20the%20marine%20copiotroph%20Alteromonas%20macleodii.%20%3Ci%3EMSystems%3C%5C%2Fi%3E%2C%20%3Ci%3E5%3C%5C%2Fi%3E%282%29.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FmSystems.00070-20%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FmSystems.00070-20%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Transcriptomic%20study%20of%20substrate-specific%20transport%20mechanisms%20for%20iron%20and%20carbon%20in%20the%20marine%20copiotroph%20Alteromonas%20macleodii%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%20E.%22%2C%22lastName%22%3A%22Manck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20L.%22%2C%22lastName%22%3A%22Espinoza%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20L.%22%2C%22lastName%22%3A%22Dupont%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22Iron%20is%20an%20essential%20micronutrient%20for%20all%20microbial%20growth%20in%20the%20marine%20environment%2C%20and%20in%20heterotrophic%20bacteria%2C%20iron%20is%20tightly%20linked%20to%20carbon%20metabolism%20due%20to%20its%20central%20role%20as%20a%20cofactor%20in%20enzymes%20of%20the%20respiratory%20chain.%20Here%2C%20we%20present%20the%20iron-%20and%20carbon-regulated%20transcriptomes%20of%20a%20representative%20marine%20copiotroph%2C%20Alteromonas%20macleodii%20ATCC%2027126%2C%20and%20characterize%20its%20cellular%20transport%20mechanisms.%20ATCC%2027126%20has%20distinct%20metabolic%20responses%20to%20iron%20and%20carbon%20limitation%20and%2C%20accordingly%2C%20uses%20distinct%20sets%20of%20TonB-dependent%20transporters%20for%20the%20acquisition%20of%20iron%20and%20carbon.%20These%20distinct%20sets%20of%20TonB-dependent%20transporters%20were%20of%20a%20similar%20number%2C%20indicating%20that%20the%20diversity%20of%20carbon%20and%20iron%20substrates%20available%20to%20ATCC%2027126%20is%20of%20a%20similar%20scale.%20For%20the%20first%20time%20in%20a%20marine%20bacterium%2C%20we%20have%20also%20identified%20six%20characteristic%20inner%20membrane%20permeases%20for%20the%20transport%20of%20siderophores%20via%20an%20ATPase-independent%20mechanism.%20An%20examination%20of%20the%20distribution%20of%20specific%20TonB-dependent%20transporters%20in%2031%20genomes%20across%20the%20genus%20Alteromonas%20points%20to%20niche%20specialization%20in%20transport%20capacity%2C%20particularly%20for%20iron.%20We%20conclude%20that%20the%20substrate-specific%20bioavailability%20of%20both%20iron%20and%20carbon%20in%20the%20marine%20environment%20will%20likely%20be%20a%20key%20control%20on%20the%20processing%20of%20organic%20matter%20through%20the%20microbial%20loop.%20IMPORTANCE%20As%20the%20major%20facilitators%20of%20the%20turnover%20of%20organic%20matter%20in%20the%20marine%20environment%2C%20the%20ability%20of%20heterotrophic%20bacteria%20to%20acquire%20specific%20compounds%20within%20the%20diverse%20range%20of%20dissolved%20organic%20matter%20will%20affect%20the%20regeneration%20of%20essential%20nutrients%20such%20as%20iron%20and%20carbon.%20TonB-dependent%20transporters%20are%20a%20prevalent%20cellular%20tool%20in%20Gram-negative%20bacteria%20that%20allow%20a%20relatively%20high-molecular-weight%20fraction%20of%20organic%20matter%20to%20be%20directly%20accessed.%20However%2C%20these%20transporters%20are%20not%20well%20characterized%20in%20marine%20bacteria%2C%20limiting%20our%20understanding%20of%20the%20flow%20of%20specific%20substrates%20through%20the%20marine%20microbial%20loop.%20Here%2C%20we%20characterize%20the%20TonB-dependent%20transporters%20responsible%20for%20iron%20and%20carbon%20acquisition%20in%20a%20representative%20marine%20copiotroph%20and%20examine%20their%20distribution%20across%20the%20genus%20Alteromonas.%20We%20provide%20evidence%20that%20substrate-specific%20bioavailability%20is%20niche%20specific%2C%20particularly%20for%20iron%20complexes%2C%20indicating%20that%20transport%20capacity%20may%20serve%20as%20a%20significant%20control%20on%20microbial%20community%20dynamics%20and%20the%20resultant%20cycling%20of%20organic%20matter.%22%2C%22date%22%3A%222020%5C%2F03%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1128%5C%2FmSystems.00070-20%22%2C%22ISSN%22%3A%222379-5077%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A17Z%22%7D%7D%2C%7B%22key%22%3A%223AKD5NW7%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Stukel%20and%20Barbeau%22%2C%22parsedDate%22%3A%222020-03%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EStukel%2C%20M.%20R.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282020%29.%20Investigating%20the%20nutrient%20landscape%20in%20a%20coastal%20upwelling%20region%20and%20its%20relationship%20to%20the%20biological%20carbon%20pump.%20%3Ci%3EGeophysical%20Research%20Letters%3C%5C%2Fi%3E%2C%20%3Ci%3E47%3C%5C%2Fi%3E%286%29.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1029%5C%2F2020gl087351%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1029%5C%2F2020gl087351%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Investigating%20the%20nutrient%20landscape%20in%20a%20coastal%20upwelling%20region%20and%20its%20relationship%20to%20the%20biological%20carbon%20pump%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20R.%22%2C%22lastName%22%3A%22Stukel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22We%20investigated%20nutrient%20patterns%20and%20their%20relationship%20to%20vertical%20carbon%20export%20using%20results%20from%2038%20Lagrangian%20experiments%20in%20the%20California%20Current%20Ecosystem.%20The%20dominant%20mode%20of%20variability%20reflected%20onshore-offshore%20nutrient%20gradients.%20A%20secondary%20mode%20of%20variability%20was%20correlated%20with%20silica%20excess%20and%20dissolved%20iron%20and%20likely%20reflects%20regional%20patterns%20of%20iron%20limitation.%20The%20biological%20carbon%20pump%20was%20enhanced%20in%20high-nutrient%20and%20Fe-stressed%20regions.%20Patterns%20in%20the%20nutrient%20landscape%20proved%20to%20be%20better%20predictors%20of%20the%20vertical%20flux%20of%20sinking%20particles%20than%20contemporaneous%20measurements%20of%20net%20primary%20production.%20Our%20results%20suggest%20an%20important%20role%20for%20Fe-stressed%20diatoms%20in%20vertical%20carbon%20flux.%20They%20also%20suggest%20that%20either%20preferential%20recycling%20of%20N%20or%20non-Redfieldian%20nutrient%20uptake%20by%20diatoms%20may%20lead%20to%20high%20PO43-%3ANO3-%20and%20Si%28OH%29%284%29%3ANO3-%20ratios%2C%20following%20export%20of%20P-%20and%20Si-enriched%20organic%20matter.%20Increased%20export%20following%20Fe%20stress%20may%20partially%20explain%20inverse%20relationships%20between%20net%20primary%20productivity%20and%20export%20efficiency.%20Plain%20Language%20Summary%20The%20productivity%20of%20marine%20ecosystems%20is%20limited%20by%20the%20availability%20of%20macronutrients%20%28nitrogen%20and%20phosphorus%29%20and%20trace%20elements%20%28iron%29%20in%20the%20sunlit%20surface%20ocean.%20The%20ocean%27s%20ability%20to%20absorb%20atmospheric%20carbon%20dioxide%20through%20the%20%5C%22biological%20carbon%20pump%5C%22%20is%20further%20constrained%20by%20the%20rates%20at%20which%20oceanic%20upwelling%20naturally%20fertilizes%20the%20surface%20ocean%20with%20%5C%22new%5C%22%20nitrogen%20contained%20in%20nutrient-rich%20deep%20water.%20We%20investigated%20patterns%20in%20nutrient%20distributions%20during%2038%20experiments%20in%20the%20California%20Current%20Ecosystem.%20In%20these%20experiments%2C%20we%20followed%20biological%20communities%20as%20they%20were%20transported%20with%20the%20currents%20and%20measured%20nutrients%2C%20primary%20productivity%2C%20and%20the%20export%20of%20organic%20carbon%20contained%20in%20sinking%20particles.%20Our%20results%20indicate%20that%20nutrient%20concentrations%20may%20be%20a%20useful%20predictor%20of%20rates%20of%20carbon%20export%20and%20that%20iron%20stress%20increased%20the%20efficiency%20with%20which%20organic%20carbon%20created%20by%20algae%20is%20transported%20to%20the%20deep%20ocean.%20This%20increased%20carbon%20export%20efficiency%20likely%20results%20from%20physiological%20changes%20within%20diatoms%20that%20lead%20to%20thicker%20silica%20shells%20relative%20to%20organic%20carbon%20content.%22%2C%22date%22%3A%222020%5C%2F03%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1029%5C%2F2020gl087351%22%2C%22ISSN%22%3A%220094-8276%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A15Z%22%7D%7D%2C%7B%22key%22%3A%223DVSPXSF%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Coale%20et%20al.%22%2C%22parsedDate%22%3A%222019-11%22%2C%22numChildren%22%3A4%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ECoale%2C%20T.%20H.%2C%20Moosburner%2C%20M.%2C%20Horak%2C%20A.%2C%20Obornik%2C%20M.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20%26amp%3B%20Allen%2C%20A.%20E.%20%282019%29.%20Reduction-dependent%20siderophore%20assimilation%20in%20a%20model%20pennate%20diatom.%20%3Ci%3EProceedings%20of%20the%20National%20Academy%20of%20Sciences%20of%20the%20United%20States%20of%20America%3C%5C%2Fi%3E%2C%20%3Ci%3E116%3C%5C%2Fi%3E%2847%29%2C%2023609%26%23x2013%3B23617.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.1907234116%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.1907234116%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Reduction-dependent%20siderophore%20assimilation%20in%20a%20model%20pennate%20diatom%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T.%20H.%22%2C%22lastName%22%3A%22Coale%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Moosburner%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Horak%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Obornik%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22Iron%20uptake%20by%20diatoms%20is%20a%20biochemical%20process%20with%20global%20biogeochemical%20implications.%20In%20large%20regions%20of%20the%20surface%20ocean%20diatoms%20are%20both%20responsible%20for%20the%20majority%20of%20primary%20production%20and%20frequently%20experiencing%20iron%20limitation%20of%20growth.%20The%20strategies%20used%20by%20these%20phytoplankton%20to%20extract%20iron%20from%20seawater%20constrain%20carbon%20flux%20into%20higher%20trophic%20levels%20and%20sequestration%20into%20sediments.%20In%20this%20study%20we%20use%20reverse%20genetic%20techniques%20to%20target%20putative%20iron-acquisition%20genes%20in%20the%20model%20pennate%20diatom%20Phaeodactylum%20tricornutum.%20We%20describe%20components%20of%20a%20reduction-dependent%20siderophore%20acquisition%20pathway%20that%20relies%20on%20a%20bacterial-derived%20receptor%20protein%20and%20provides%20a%20viable%20alternative%20to%20inorganic%20iron%20uptake%20under%20certain%20conditions.%20This%20form%20of%20iron%20uptake%20entails%20a%20close%20association%20between%20diatoms%20and%20siderophore-producing%20organisms%20during%20low-iron%20conditions.%20Homologs%20of%20these%20proteins%20are%20found%20distributed%20across%20diatom%20lineages%2C%20suggesting%20the%20significance%20of%20siderophore%20utilization%20by%20diatoms%20in%20the%20marine%20environment.%20Evaluation%20of%20specific%20proteins%20enables%20us%20to%20confirm%20independent%20iron-acquisition%20pathways%20in%20diatoms%20and%20characterize%20their%20preferred%20substrates.%20These%20findings%20refine%20our%20mechanistic%20understanding%20of%20the%20multiple%20iron-uptake%20systems%20used%20by%20diatoms%20and%20help%20us%20better%20predict%20the%20influence%20of%20iron%20speciation%20on%20taxa-specific%20iron%20bioavailability.%22%2C%22date%22%3A%222019%5C%2F11%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1073%5C%2Fpnas.1907234116%22%2C%22ISSN%22%3A%220027-8424%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%2C%2286H9SNJB%22%5D%2C%22dateModified%22%3A%222022-08-05T16%3A19%3A47Z%22%7D%7D%2C%7B%22key%22%3A%22S6R9U35R%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Meskhidze%20et%20al.%22%2C%22parsedDate%22%3A%222019-11%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMeskhidze%2C%20N.%2C%20Volker%2C%20C.%2C%20Al-Abadleh%2C%20H.%20A.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%2C%20Bressac%2C%20M.%2C%20Buck%2C%20C.%2C%20Bundy%2C%20R.%20M.%2C%20Croot%2C%20P.%2C%20Feng%2C%20Y.%2C%20Ito%2C%20A.%2C%20Johansen%2C%20A.%20M.%2C%20Landing%2C%20W.%20M.%2C%20Mao%2C%20J.%20Q.%2C%20Myriokefalitakis%2C%20S.%2C%20Ohnemus%2C%20D.%2C%20Pasquier%2C%20B.%2C%20%26amp%3B%20Ye%2C%20Y.%20%282019%29.%20Perspective%20on%20identifying%20and%20characterizing%20the%20processes%20controlling%20iron%20speciation%20and%20residence%20time%20at%20the%20atmosphere-ocean%20interface.%20%3Ci%3EMarine%20Chemistry%3C%5C%2Fi%3E%2C%20%3Ci%3E217%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2019.103704%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2019.103704%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Perspective%20on%20identifying%20and%20characterizing%20the%20processes%20controlling%20iron%20speciation%20and%20residence%20time%20at%20the%20atmosphere-ocean%20interface%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22N.%22%2C%22lastName%22%3A%22Meskhidze%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%22%2C%22lastName%22%3A%22Volker%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22H.%20A.%22%2C%22lastName%22%3A%22Al-Abadleh%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Bressac%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%22%2C%22lastName%22%3A%22Buck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Croot%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Y.%22%2C%22lastName%22%3A%22Feng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Ito%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20M.%22%2C%22lastName%22%3A%22Johansen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22W.%20M.%22%2C%22lastName%22%3A%22Landing%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20Q.%22%2C%22lastName%22%3A%22Mao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Myriokefalitakis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%22%2C%22lastName%22%3A%22Ohnemus%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%22%2C%22lastName%22%3A%22Pasquier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Y.%22%2C%22lastName%22%3A%22Ye%22%7D%5D%2C%22abstractNote%22%3A%22It%20is%20well%20recognized%20that%20the%20atmospheric%20deposition%20of%20iron%20%28Fe%29%20affects%20ocean%20productivity%2C%20atmospheric%20CO2%20uptake%2C%20ecosystem%20diversity%2C%20and%20overall%20climate.%20Despite%20significant%20advances%20in%20measurement%20techniques%20and%20modeling%20efforts%2C%20discrepancies%20persist%20between%20observations%20and%20models%20that%20hinder%20accurate%20predictions%20of%20processes%20and%20their%20global%20effects.%20Here%2C%20we%20provide%20an%20assessment%20report%20on%20where%20the%20current%20state%20of%20knowledge%20is%20and%20where%20future%20research%20emphasis%20would%20have%20the%20highest%20impact%20in%20furthering%20the%20field%20of%20Fe%20atmosphere-ocean%20biogeochemical%20cycle.%20These%20results%20were%20determined%20through%20consensus%20reached%20by%20diverse%20researchers%20from%20the%20oceanographic%20and%20atmospheric%20science%20communities%20with%20backgrounds%20in%20laboratory%20and%20in%20situ%20measurements%2C%20modeling%2C%20and%20remote%20sensing.%20We%20discuss%20i%29%20novel%20measurement%20methodologies%20and%20instrumentation%20that%20allow%20detection%20and%20speciation%20of%20different%20forms%20and%20oxidation%20states%20of%20Fe%20in%20deliquesced%20mineral%20aerosol%2C%20cloud%5C%2Frainwater%2C%20and%20seawater%3B%20ii%29%20oceanic%20models%20that%20treat%20Fe%20cycling%20with%20several%20external%20sources%20and%20sinks%2C%20dissolved%2C%20colloidal%2C%20particulate%2C%20inorganic%2C%20and%20organic%20ligand-complexed%20forms%20of%20Fe%2C%20as%20well%20as%20Fe%20in%20detritus%20and%20phytoplankton%3B%20and%20iii%29%20atmospheric%20models%20that%20consider%20natural%20and%20anthropogenic%20sources%20of%20Fe%2C%20mobilization%20of%20Fe%20in%20mineral%20aerosols%20due%20to%20the%20dissolution%20of%20Fe-oxides%20and%20Fe-substituted%20aluminosilicates%20through%20proton-promoted%2C%20organic%20ligand-promoted%2C%20and%20photo-reductive%20mechanisms.%20In%20addition%2C%20the%20study%20identifies%20existing%20challenges%20and%20disconnects%20%28both%20fundamental%20and%20methodological%29%20such%20as%20i%29%20inconsistencies%20in%20Fe%20nomenclature%20and%20the%20definition%20of%20bioavailable%20Fe%20between%20oceanic%20and%20atmospheric%20disciplines%2C%20and%20ii%29%20the%20lack%20of%20characterization%20of%20the%20processes%20controlling%20Fe%20speciation%20and%20residence%20time%20at%20the%20atmosphere-ocean%20interface.%20Such%20challenges%20are%20undoubtedly%20caused%20by%20extremely%20low%20concentrations%2C%20short%20lifetime%2C%20and%20the%20myriad%20of%20physical%2C%20%28photo%29chemical%2C%20and%20biological%20processes%20affecting%20global%20biogeochemical%20cycling%20of%20Fe.%20However%2C%20we%20also%20argue%20that%20the%20historical%20division%20%28separate%20treatment%20of%20Fe%20biogeochemistry%20in%20oceanic%20and%20atmospheric%20disciplines%29%20and%20the%20classical%20funding%20structures%20%28that%20often%20create%20obstacles%20for%20transdisciplinary%20collaboration%29%20are%20also%20hampering%20the%20advancement%20of%20knowledge%20in%20the%20field.%20Finally%2C%20the%20study%20provides%20some%20specific%20ideas%20and%20guidelines%20for%20laboratory%20studies%2C%20field%20measurements%2C%20and%20modeling%20research%20required%20for%20improved%20characterization%20of%20global%20biogeochemical%20cycling%20of%20Fe%20in%20relationship%20with%20other%20trace%20elements%20and%20essential%20nutrients.%20The%20report%20is%20intended%20to%20aid%20scientists%20in%20their%20work%20related%20to%20Fe%20biogeochemistry%20as%20well%20as%20program%20managers%20at%20the%20relevant%20funding%20agencies.%22%2C%22date%22%3A%222019%5C%2F11%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.marchem.2019.103704%22%2C%22ISSN%22%3A%220304-4203%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A16Z%22%7D%7D%2C%7B%22key%22%3A%22LLN4ATWC%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Jiang%20et%20al.%22%2C%22parsedDate%22%3A%222019-08%22%2C%22numChildren%22%3A8%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EJiang%2C%20M.%2C%20Measures%2C%20C.%20I.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20Charette%2C%20M.%20A.%2C%20Gille%2C%20S.%20T.%2C%20Hatta%2C%20M.%2C%20Kahru%2C%20M.%2C%20Mitchell%2C%20B.%20G.%2C%20Garabato%2C%20A.%20C.%20N.%2C%20Reiss%2C%20C.%2C%20Selph%2C%20K.%2C%20%26amp%3B%20Zhou%2C%20M.%20%282019%29.%20Fe%20sources%20and%20transport%20from%20the%20Antarctic%20Peninsula%20shelf%20to%20the%20southern%20Scotia%20Sea.%20%3Ci%3EDeep-Sea%20Research%20Part%20I-Oceanographic%20Research%20Papers%3C%5C%2Fi%3E%2C%20%3Ci%3E150%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.dsr.2019.06.006%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.dsr.2019.06.006%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Fe%20sources%20and%20transport%20from%20the%20Antarctic%20Peninsula%20shelf%20to%20the%20southern%20Scotia%20Sea%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Jiang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20I.%22%2C%22lastName%22%3A%22Measures%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20A.%22%2C%22lastName%22%3A%22Charette%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%20T.%22%2C%22lastName%22%3A%22Gille%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Hatta%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Kahru%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20G.%22%2C%22lastName%22%3A%22Mitchell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20C.%20N.%22%2C%22lastName%22%3A%22Garabato%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%22%2C%22lastName%22%3A%22Reiss%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Selph%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Zhou%22%7D%5D%2C%22abstractNote%22%3A%22The%20Antarctic%20Peninsula%20%28AP%29%20shelf%20is%20an%20important%20source%20of%20dissolved%20iron%20%28Fe%29%20to%20the%20upper%20ocean%20in%20the%20southern%20Scotia%20Sea%2C%20one%20of%20the%20most%20productive%20regions%20of%20the%20Southern%20Ocean.%20Here%20we%20present%20results%20from%20a%20four-year%20%282003-2006%29%20numerical%20simulation%20using%20a%20regional%20coupled%20physical-biogeochemical%20model%20to%20assess%20the%20Fe%20sources%20and%20transport%20on%20the%20AP%20shelf%20and%20toward%20the%20southern%20Scotia%20Sea.%20The%20model%20was%20validated%20with%20a%20suite%20of%20data%20derived%20from%20in%20situ%20surveys%20and%20remote%20sensing.%20Model%20results%20indicate%20that%20sediments%20in%20the%20AP%20shelf%20and%20the%20South%20Orkney%20Plateau%20%28SOP%29%20provide%20the%20dominant%20source%20of%20Fe%20to%20the%20upper%20500%20m%20in%20the%20southern%20Scotia%20Sea.%20Additional%20Fe%20inputs%20to%20the%20region%20are%20associated%20with%20the%20Antarctic%20Circumpolar%20Current%20%28ACC%29%20and%20the%20northern%20limb%20of%20the%20Weddell%20Gyre%2C%20deep-ocean%20sediment%20sources%2C%20dust%20deposition%2C%20and%20icebergs.%20Fe%20on%20the%20AP%20shelf%20originates%20primarily%20from%20sediments%20on%20the%20relatively%20shallow%20inner%20shelf%20and%20is%20directly%20injected%20into%20the%20water%20column%20and%20subsequently%20transported%20toward%20Elephant%20Island%20by%20the%20confluent%20shelf%20currents.%20Off-shelf%20Fe%20export%20is%20primarily%20through%20entrainment%20of%20shelf%20waters%20by%20the%20ACC%27s%20Southern%20Boundary%20frontal%20jet%20along%20the%20northern%20edge%20of%20the%20AP%20shelf%2C%20the%20Hesperides%20Trough%2C%20and%20the%20SOP%20shelf.%20About%2070%25%20of%20the%20off-shelf%20export%20takes%20place%20below%20the%20surface%20mixed%20layer%2C%20and%20is%20subsequently%20re-supplied%20to%20the%20euphotic%20zone%20through%20vertical%20mixing%2C%20mainly%20during%20austral%20fall%20and%20winter.%20The%20exported%20shelf-derived%20Fe%20is%20then%20advected%20downstream%20by%20the%20ACC%20and%20Weddell%20Gyre%20and%20spread%20over%20the%20southern%20and%20eastern%20Scotia%20Seas.%20Taken%20together%2C%20shelf%20Fe%20export%20witin%20top%20500%20m%20meets%20nearly%20all%20of%20the%20Fe%20demand%20of%20phytoplankton%20photosynthesis%20in%20the%20southern%20Scotia%20Sea.%20Waters%20with%20elevated%20Fe%20concentrations%20in%20the%20Scotia%20Sea%20are%20largely%20restricted%20to%20south%20of%20the%20Southern%20ACC%20Front.%22%2C%22date%22%3A%222019%5C%2F08%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.dsr.2019.06.006%22%2C%22ISSN%22%3A%220967-0637%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22WQ4Y333C%22%2C%22MWYMG4GN%22%2C%22DPYPUWVE%22%2C%22GAZRXK5T%22%5D%2C%22dateModified%22%3A%222022-10-25T17%3A53%3A42Z%22%7D%7D%2C%7B%22key%22%3A%22LC6XF5BT%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Stukel%20et%20al.%22%2C%22parsedDate%22%3A%222019-05%22%2C%22numChildren%22%3A10%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EStukel%2C%20M.%20R.%2C%20Kelly%2C%20T.%20B.%2C%20Aluwihare%2C%20L.%20I.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20Goericke%2C%20R.%2C%20Krause%2C%20J.%20W.%2C%20Landry%2C%20M.%20R.%2C%20%26amp%3B%20Ohman%2C%20M.%20D.%20%282019%29.%20The%20Carbon%3A%28234%29Thorium%20ratios%20of%20sinking%20particles%20in%20the%20California%20current%20ecosystem%201%3A%20relationships%20with%20plankton%20ecosystem%20dynamics.%20%3Ci%3EMarine%20Chemistry%3C%5C%2Fi%3E%2C%20%3Ci%3E212%3C%5C%2Fi%3E%2C%201%26%23x2013%3B15.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2019.01.003%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2019.01.003%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20Carbon%3A%28234%29Thorium%20ratios%20of%20sinking%20particles%20in%20the%20California%20current%20ecosystem%201%3A%20relationships%20with%20plankton%20ecosystem%20dynamics%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20R.%22%2C%22lastName%22%3A%22Stukel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T.%20B.%22%2C%22lastName%22%3A%22Kelly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%20I.%22%2C%22lastName%22%3A%22Aluwihare%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Goericke%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20W.%22%2C%22lastName%22%3A%22Krause%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20R.%22%2C%22lastName%22%3A%22Landry%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20D.%22%2C%22lastName%22%3A%22Ohman%22%7D%5D%2C%22abstractNote%22%3A%22We%20investigated%20variability%20in%20the%20C%3ATh-234%20ratio%20of%20sinking%20particles%20and%20its%20relationship%20to%20changing%20water%20column%20characteristics%20and%20plankton%20ecological%20dynamics%20during%2029%20Lagrangian%20experiments%20conducted%20on%20six%20cruises%20of%20the%20California%20Current%20Ecosystem%20Long-Term%20Ecological%20Research%20%28CCE-LTER%29%20Program.%20C%3ATh-234%20ratios%20of%20sinking%20particles%20collected%20by%20a%20surface-tethered%20sediment%20trap%20%28%28CThST%29-Th-%3A234%29%20varied%20from%202.3%20to%2020.5%20mu%20mol%20C%20dpm%28-1%29%20over%20a%20depth%20range%20of%2047-150%20m.%20C%3ATh-234%28ST%29%20was%20significantly%20greater%20%28by%20a%20factor%20of%201.8%29%20than%20C%3ATh-234%20ratios%20of%20suspended%20%3E%2051-mu%20m%20particles%20collected%20in%20the%20same%20water%20parcels%20with%20in%20situ%20pumps.%20C%3ATh-234%20ratios%20of%20large%20%28%3E%20200-mu%20m%29%20sinking%20particles%20also%20exceeded%20those%20of%20smaller%20sinking%20particles.%20C%3ATh-234%28ST%29%20decreased%20with%20depth%20from%20the%20base%20of%20the%20euphotic%20zone%20through%20the%20upper%20twilight%20zone.%20C%3ATh-234%28ST%29%20was%20positively%20correlated%20with%20several%20indices%20of%20ecosystem%20productivity%20including%20particulate%20organic%20carbon%20%28POC%29%20and%20chlorophyll%20%28Chl%29%20concentrations%2C%20mesozooplankton%20biomass%2C%20and%20the%20fraction%20of%20Chl%20%3E%2020-mu%20m.%20Principal%20component%20analysis%20and%20multiple%20linear%20regression%20suggested%20that%20decaying%20phytoplankton%20blooms%20exhibited%20higher%20C%3ATh-234%28ST%29%20than%20actively%20growing%20blooms%20at%20similar%20biomass%20levels.%20C%3ATh-234%28ST%29%20was%20positively%20correlated%20with%20indices%20of%20the%20fractional%20contribution%20of%20fecal%20pellets%20in%20sediment%20traps%20when%20the%20proportion%20of%20fecal%20pellets%20was%20low%20in%20the%20traps%2C%20likely%20because%20of%20a%20correlation%20between%20mesozooplankton%20biomass%20and%20other%20indices%20of%20ecosystem%20productivity.%20However%2C%20when%20fecal%20pellets%20were%20a%20more%20important%20component%20of%20sinking%20material%2C%20C%3ATh-234%28ST%29%20decreased%20with%20increasing%20fecal%20pellet%20content.%20C%3ATh-234%28ST%29%20was%20also%20positively%20correlated%20with%20the%20Si%3AC%20ratio%20of%20sinking%20particles.%20Across%20the%20dataset%20%28and%20across%20depths%29%20a%20strong%20correlation%20was%20found%20between%20C%3ATh-234%28ST%29%20and%20the%20ratio%20of%20vertically-integrated%20POC%20to%20vertically-integrated%20total%20water%20column%20Th-234%20%28C-v%3ATh-234%28tot%29%29.%20A%20mechanistic%20one-layer%2C%20two-box%20model%20of%20thorium%20sorption%20and%20desorption%20was%20invoked%20to%20explain%20this%20correlation.%20Two%20empirical%20models%20%28one%20using%20C-v%3ATh-234%28tot%29%3B%20one%20using%20depth%20and%20vertically-integrated%20Chl%29%20were%20developed%20to%20predict%20C%3ATh-234%20ratios%20in%20this%20coastal%20upwelling%20biome.%20The%20former%20regression%20%28log%2810%29%28C%3ATh-234%28ST%29%29%20%3D%200.43%20x%20log%2810%29%28C-v%3ATh-234%28tot%29%29%20%2B%200.53%29%20was%20found%20to%20also%20be%20a%20reasonable%20predictor%20for%20C%3ATh-234%28ST%29%20from%20diverse%20regions%20including%20the%20Southern%20Ocean%2C%20Sargasso%20Sea%2C%20Subarctic%20North%20Pacific%2C%20and%20Eastern%20Tropical%20North%20Pacific.%22%2C%22date%22%3A%222019%5C%2F05%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.marchem.2019.01.003%22%2C%22ISSN%22%3A%220304-4203%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22QIYZ9CQ7%22%2C%22MWYMG4GN%22%2C%22FWE37XSJ%22%2C%22WJTCAXQW%22%2C%22PY4MY9R2%22%5D%2C%22dateModified%22%3A%222022-10-25T17%3A54%3A56Z%22%7D%7D%2C%7B%22key%22%3A%226F6EVUB9%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Chappell%20et%20al.%22%2C%22parsedDate%22%3A%222019-01%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EChappell%2C%20P.%20D.%2C%20Armbrust%2C%20E.%20V.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20Bundy%2C%20R.%20M.%2C%20Moffett%2C%20J.%20W.%2C%20Vedamati%2C%20J.%2C%20%26amp%3B%20Jenkins%2C%20B.%20D.%20%282019%29.%20Patterns%20of%20diatom%20diversity%20correlate%20with%20dissolved%20trace%20metal%20concentrations%20and%20longitudinal%20position%20in%20the%20northeast%20Pacific%20coastal-offshore%20transition%20zone.%20%3Ci%3EMarine%20Ecology%20Progress%20Series%3C%5C%2Fi%3E%2C%20%3Ci%3E609%3C%5C%2Fi%3E%2C%2069%26%23x2013%3B86.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3354%5C%2Fmeps12810%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3354%5C%2Fmeps12810%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Patterns%20of%20diatom%20diversity%20correlate%20with%20dissolved%20trace%20metal%20concentrations%20and%20longitudinal%20position%20in%20the%20northeast%20Pacific%20coastal-offshore%20transition%20zone%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%20D.%22%2C%22lastName%22%3A%22Chappell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20V.%22%2C%22lastName%22%3A%22Armbrust%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20W.%22%2C%22lastName%22%3A%22Moffett%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Vedamati%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20D.%22%2C%22lastName%22%3A%22Jenkins%22%7D%5D%2C%22abstractNote%22%3A%22Diatoms%20are%20important%20primary%20producers%20in%20the%20northeast%20Pacific%20Ocean%2C%20with%20their%20productivity%20closely%20linked%20to%20pulses%20of%20trace%20elements%20in%20the%20western%20high%20nitrate%2C%20low%20chlorophyll%20%28HNLC%29%20region%20of%20the%20oceanographic%20time%20series%20transect%20%27Line%20P.%27%20Recently%2C%20the%20coastal-HNLC%20transition%20zone%20of%20the%20Line%20P%20transect%20was%20identified%20as%20a%20hotspot%20of%20phytoplankton%20productivity%2C%20potentially%20controlled%20by%20a%20combination%20of%20trace%20element%20and%20macronutrient%20concentrations.%20Here%20we%20describe%20diatom%20community%20composition%20in%20the%20eastern%20Line%20P%20transect%2C%20including%20the%20coastal-%20HNLC%20transition%20zone%2C%20with%20a%20method%20using%20high-throughput%20sequencing%20of%20diatom%2018S%20gene%20amplicons.%20We%20identified%20significant%20correlations%20between%20shifting%20diatom%20community%20composition%20and%20longitude%20combined%20with%20concentrations%20of%20dissolved%20copper%20and%202%20other%20dissolved%20trace%20metals%20%28dissolved%20Fe%20%5BdFe%5D%20and%5C%2For%20dissolved%20zinc%29%20and%5C%2For%20a%20physical%20factor%20%28salinity%20or%20density%29.%20None%20of%20these%20variables%20on%20its%20own%20was%20significantly%20correlated%20with%20shifts%20in%20community%20composition%2C%20and%203%20of%20the%20factors%20%28dFe%2C%20salinity%2C%20and%20density%29%20correlated%20with%20one%20another.%20Longitude%20could%20incorporate%20multiple%20factors%20that%20may%20influence%20diatom%20communities%2C%20including%20distance%20from%20shore%2C%20proximity%20of%20sampling%20stations%2C%20and%20an%20integration%20of%20previous%20pulses%20of%20macro-%20and%20micro-nutrients.%20We%20also%20evaluated%20in%20situ%20Fe%20limitation%20of%20the%20diatom%20Thalassiosira%20oceanica%20using%20a%20quantitative%20reverse-transcription%20polymerase%20chain%20reaction%20method%2C%20and%20found%20biological%20evidence%20of%20Fe%20stress%20in%20samples%20from%20the%20coastal-HNLC%20transition%20zone.%20Combined%2C%20our%20results%20support%20a%20prior%20hypothesis%20that%20dissolved%20trace%20metals%20as%20well%20as%20longitudinal%20distance%20may%20be%20important%20to%20diatom%20diversity%20in%20the%20coastal-HNLC%20transition%20zone%20of%20the%20Line%20P%20transect.%22%2C%22date%22%3A%222019%5C%2F01%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.3354%5C%2Fmeps12810%22%2C%22ISSN%22%3A%220171-8630%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A16Z%22%7D%7D%2C%7B%22key%22%3A%22F6FFC26Y%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hogle%20et%20al.%22%2C%22parsedDate%22%3A%222018-12%22%2C%22numChildren%22%3A4%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHogle%2C%20S.%20L.%2C%20Dupont%2C%20C.%20L.%2C%20Hopkinson%2C%20B.%20M.%2C%20King%2C%20A.%20L.%2C%20Buck%2C%20K.%20N.%2C%20Roe%2C%20K.%20L.%2C%20Stuart%2C%20R.%20K.%2C%20Allen%2C%20A.%20E.%2C%20Mann%2C%20E.%20L.%2C%20Johnson%2C%20Z.%20I.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282018%29.%20Pervasive%20iron%20limitation%20at%20subsurface%20chlorophyll%20maxima%20of%20the%20California%20Current.%20%3Ci%3EProceedings%20of%20the%20National%20Academy%20of%20Sciences%20of%20the%20United%20States%20of%20America%3C%5C%2Fi%3E%2C%20%3Ci%3E115%3C%5C%2Fi%3E%2852%29%2C%2013300%26%23x2013%3B13305.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.1813192115%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.1813192115%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Pervasive%20iron%20limitation%20at%20subsurface%20chlorophyll%20maxima%20of%20the%20California%20Current%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%20L.%22%2C%22lastName%22%3A%22Hogle%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20L.%22%2C%22lastName%22%3A%22Dupont%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20M.%22%2C%22lastName%22%3A%22Hopkinson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20L.%22%2C%22lastName%22%3A%22King%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20N.%22%2C%22lastName%22%3A%22Buck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20L.%22%2C%22lastName%22%3A%22Roe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20K.%22%2C%22lastName%22%3A%22Stuart%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20L.%22%2C%22lastName%22%3A%22Mann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Z.%20I.%22%2C%22lastName%22%3A%22Johnson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22Subsurface%20chlorophyll%20maximum%20layers%20%28SCMLs%29%20are%20nearly%20ubiquitous%20in%20stratified%20water%20columns%20and%20exist%20at%20horizontal%20scales%20ranging%20from%20the%20submesoscale%20to%20the%20extent%20of%20oligotrophic%20gyres.%20These%20layers%20of%20heightened%20chlorophyll%20and%5C%2For%20phytoplankton%20concentrations%20are%20generally%20thought%20to%20be%20a%20consequence%20of%20a%20balance%20between%20light%20energy%20from%20above%20and%20a%20limiting%20nutrient%20flux%20from%20below%2C%20typically%20nitrate%20%28NO3%29.%20Here%20we%20present%20multiple%20lines%20of%20evidence%20demonstrating%20that%20iron%20%28Fe%29%20limits%20or%20with%20light%20colimits%20phytoplankton%20communities%20in%20SCMLs%20along%20a%20primary%20productivity%20gradient%20from%20coastal%20to%20oligotrophic%20offshore%20waters%20in%20the%20southern%20California%20Current%20ecosystem.%20SCML%20phytoplankton%20responded%20markedly%20to%20added%20Fe%20or%20Fe%5C%2Flight%20in%20experimental%20incubations%20and%20transcripts%20of%20diatom%20and%20picoeukaryote%20Fe%20stress%20genes%20were%20strikingly%20abundant%20in%20SCML%20metatranscriptomes.%20Using%20a%20biogeochemical%20proxy%20with%20data%20from%20a%2040-y%20time%20series%2C%20we%20find%20that%20diatoms%20growing%20in%20California%20Current%20SCMLs%20are%20persistently%20Fe%20deficient%20during%20the%20spring%20and%20summer%20growing%20season.%20We%20also%20find%20that%20the%20spatial%20extent%20of%20Fe%20deficiency%20within%20California%20Current%20SCMLs%20has%20significantly%20increased%20over%20the%20last%2025%20y%20in%20line%20with%20a%20regional%20climate%20index.%20Finally%2C%20we%20show%20that%20diatom%20Fe%20deficiency%20may%20be%20common%20in%20the%20subsurface%20of%20major%20upwelling%20zones%20worldwide.%20Our%20results%20have%20important%20implications%20for%20our%20understanding%20of%20the%20biogeochemical%20consequences%20of%20marine%20SCML%20formation%20and%20maintenance.%22%2C%22date%22%3A%222018%5C%2F12%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1073%5C%2Fpnas.1813192115%22%2C%22ISSN%22%3A%220027-8424%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%2C%2286H9SNJB%22%5D%2C%22dateModified%22%3A%222022-09-22T23%3A35%3A31Z%22%7D%7D%2C%7B%22key%22%3A%222ZXE9G3D%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22McQuaid%20et%20al.%22%2C%22parsedDate%22%3A%222018-03%22%2C%22numChildren%22%3A5%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMcQuaid%2C%20J.%20B.%2C%20Kustka%2C%20A.%20B.%2C%20Oborn%26%23xED%3Bk%2C%20M.%2C%20Hor%26%23xE1%3Bk%2C%20A.%2C%20McCrow%2C%20J.%20P.%2C%20Karas%2C%20B.%20J.%2C%20Zheng%2C%20H.%2C%20Kindeberg%2C%20T.%2C%20Andersson%2C%20A.%20J.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20%26amp%3B%20Allen%2C%20A.%20E.%20%282018%29.%20Carbonate-sensitive%20phytotransferrin%20controls%20high-affinity%20iron%20uptake%20in%20diatoms.%20%3Ci%3ENature%3C%5C%2Fi%3E%2C%20%3Ci%3E555%3C%5C%2Fi%3E%2C%20534.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fnature25982%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fnature25982%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Carbonate-sensitive%20phytotransferrin%20controls%20high-affinity%20iron%20uptake%20in%20diatoms%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeffrey%20B.%22%2C%22lastName%22%3A%22McQuaid%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Adam%20B.%22%2C%22lastName%22%3A%22Kustka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Miroslav%22%2C%22lastName%22%3A%22Oborn%5Cu00edk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ale%5Cu0161%22%2C%22lastName%22%3A%22Hor%5Cu00e1k%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22John%20P.%22%2C%22lastName%22%3A%22McCrow%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bogumil%20J.%22%2C%22lastName%22%3A%22Karas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hong%22%2C%22lastName%22%3A%22Zheng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Theodor%22%2C%22lastName%22%3A%22Kindeberg%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andreas%20J.%22%2C%22lastName%22%3A%22Andersson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katherine%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22In%20vast%20areas%20of%20the%20ocean%2C%20the%20scarcity%20of%20iron%20controls%20the%20growth%20and%20productivity%20of%20phytoplankton1%2C2.%20Although%20most%20dissolved%20iron%20in%20the%20marine%20environment%20is%20complexed%20with%20organic%20molecules3%2C%20picomolar%20amounts%20of%20labile%20inorganic%20iron%20species%20%28labile%20iron%29%20are%20maintained%20within%20the%20euphotic%20zone4%20and%20serve%20as%20an%20important%20source%20of%20iron%20for%20eukaryotic%20phytoplankton%20and%20particularly%20for%20diatoms5.%20Genome-enabled%20studies%20of%20labile%20iron%20utilization%20by%20diatoms%20have%20previously%20revealed%20novel%20iron-responsive%20transcripts6%2C7%2C%20including%20the%20ferric%20iron-concentrating%20protein%20ISIP2A8%2C%20but%20the%20mechanism%20behind%20the%20acquisition%20of%20picomolar%20labile%20iron%20remains%20unknown.%20Here%20we%20show%20that%20ISIP2A%20is%20a%20phytotransferrin%20that%20independently%20and%20convergently%20evolved%20carbonate%20ion-coordinated%20ferric%20iron%20binding.%20Deletion%20of%20ISIP2A%20disrupts%20high-affinity%20iron%20uptake%20in%20the%20diatom%20Phaeodactylum%20tricornutum%2C%20and%20uptake%20is%20restored%20by%20complementation%20with%20human%20transferrin.%20ISIP2A%20is%20internalized%20by%20endocytosis%2C%20and%20manipulation%20of%20the%20seawater%20carbonic%20acid%20system%20reveals%20a%20second-order%20dependence%20on%20the%20concentrations%20of%20labile%20iron%20and%20carbonate%20ions.%20In%20P.%20tricornutum%2C%20the%20synergistic%20interaction%20of%20labile%20iron%20and%20carbonate%20ions%20occurs%20at%20environmentally%20relevant%20concentrations%2C%20revealing%20that%20carbonate%20availability%20co-limits%20iron%20uptake.%20Phytotransferrin%20sequences%20have%20a%20broad%20taxonomic%20distribution8%20and%20are%20abundant%20in%20marine%20environmental%20genomic%20datasets9%2C10%2C%20suggesting%20that%20acidification-driven%20declines%20in%20the%20concentration%20of%20seawater%20carbonate%20ions%20will%20have%20a%20negative%20effect%20on%20this%20globally%20important%20eukaryotic%20iron%20acquisition%20mechanism.%22%2C%22date%22%3A%222018%5C%2F03%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fnature25982%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%2C%2286H9SNJB%22%2C%22R2U424Z5%22%5D%2C%22dateModified%22%3A%222022-11-21T17%3A57%3A55Z%22%7D%7D%2C%7B%22key%22%3A%22Y53D536J%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Stuart%20et%20al.%22%2C%22parsedDate%22%3A%222017-02%22%2C%22numChildren%22%3A4%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EStuart%2C%20R.%20K.%2C%20Bundy%2C%20R.%2C%20Buck%2C%20K.%2C%20Ghassemain%2C%20M.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%2C%20%26amp%3B%20Palenik%2C%20B.%20%282017%29.%20Copper%20toxicity%20response%20influences%20mesotrophic%20Synechococcus%20community%20structure.%20%3Ci%3EEnvironmental%20Microbiology%3C%5C%2Fi%3E%2C%20%3Ci%3E19%3C%5C%2Fi%3E%282%29%2C%20756%26%23x2013%3B769.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2F1462-2920.13630%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2F1462-2920.13630%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Copper%20toxicity%20response%20influences%20mesotrophic%20Synechococcus%20community%20structure%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20K.%22%2C%22lastName%22%3A%22Stuart%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Buck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Ghassemain%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%22%2C%22lastName%22%3A%22Palenik%22%7D%5D%2C%22abstractNote%22%3A%22Picocyanobacteria%20from%20the%20genus%20Synechococcus%20are%20ubiquitous%20in%20ocean%20waters.%20Their%20phylogenetic%20and%20genomic%20diversity%20suggests%20ecological%20niche%20differentiation%2C%20but%20the%20selective%20forces%20influencing%20this%20are%20not%20well%20defined.%20Marine%20picocyanobacteria%20are%20sensitive%20to%20Cu%20toxicity%2C%20so%20adaptations%20to%20this%20stress%20could%20represent%20a%20selective%20force%20within%2C%20and%20between%2C%20species%27%2C%20also%20known%20as%20clades.%20Here%2C%20we%20compared%20Cu%20stress%20responses%20in%20cultures%20and%20natural%20populations%20of%20marine%20Synechococcus%20from%20two%20co-occurring%20major%20mesotrophic%20clades%20%28I%20and%20IV%29.%20Using%20custom%20microarrays%20and%20proteomics%20to%20characterize%20expression%20responses%20to%20Cu%20in%20the%20lab%20and%20field%2C%20we%20found%20evidence%20for%20a%20general%20stress%20regulon%20in%20marine%20Synechococcus.%20However%2C%20the%20two%20clades%20also%20exhibited%20distinct%20responses%20to%20copper.%20The%20Clade%20I%20representative%20induced%20expression%20of%20genomic%20island%20genes%20in%20cultures%20and%20Southern%20California%20Bight%20populations%2C%20while%20the%20Clade%20IV%20representative%20downregulated%20Fe-limitation%20proteins.%20Copper%20incubation%20experiments%20suggest%20that%20Clade%20IV%20populations%20may%20harbour%20stress-tolerant%20subgroups%2C%20and%20thus%20fitness%20tradeoffs%20may%20govern%20Cu-tolerant%20strain%20distributions.%20This%20work%20demonstrates%20that%20Synechococcus%20has%20distinct%20adaptive%20strategies%20to%20deal%20with%20Cu%20toxicity%20at%20both%20the%20clade%20and%20subclade%20level%2C%20implying%20that%20metal%20toxicity%20and%20stress%20response%20adaptations%20represent%20an%20important%20selective%20force%20for%20influencing%20diversity%20within%20marine%20Synechococcus%20populations.%22%2C%22date%22%3A%222017%5C%2F02%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1111%5C%2F1462-2920.13630%22%2C%22ISSN%22%3A%221462-2912%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%2C%22BSCQ9CW7%22%5D%2C%22dateModified%22%3A%222023-04-10T22%3A50%3A10Z%22%7D%7D%2C%7B%22key%22%3A%2262NJJWSH%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Stukel%20et%20al.%22%2C%22parsedDate%22%3A%222017-02%22%2C%22numChildren%22%3A12%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EStukel%2C%20M.%20R.%2C%20Aluwihare%2C%20L.%20I.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20Chekalyuk%2C%20A.%20M.%2C%20Goericke%2C%20R.%2C%20Miller%2C%20A.%20J.%2C%20Ohman%2C%20M.%20D.%2C%20Ruacho%2C%20A.%2C%20Song%2C%20H.%2C%20Stephens%2C%20B.%20M.%2C%20%26amp%3B%20Landry%2C%20M.%20R.%20%282017%29.%20Mesoscale%20ocean%20fronts%20enhance%20carbon%20export%20due%20to%20gravitational%20sinking%20and%20subduction.%20%3Ci%3EProceedings%20of%20the%20National%20Academy%20of%20Sciences%20of%20the%20United%20States%20of%20America%3C%5C%2Fi%3E%2C%20%3Ci%3E114%3C%5C%2Fi%3E%286%29%2C%201252%26%23x2013%3B1257.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.1609435114%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.1609435114%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Mesoscale%20ocean%20fronts%20enhance%20carbon%20export%20due%20to%20gravitational%20sinking%20and%20subduction%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20R.%22%2C%22lastName%22%3A%22Stukel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%20I.%22%2C%22lastName%22%3A%22Aluwihare%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20M.%22%2C%22lastName%22%3A%22Chekalyuk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Goericke%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20J.%22%2C%22lastName%22%3A%22Miller%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20D.%22%2C%22lastName%22%3A%22Ohman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Ruacho%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22H.%22%2C%22lastName%22%3A%22Song%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20M.%22%2C%22lastName%22%3A%22Stephens%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20R.%22%2C%22lastName%22%3A%22Landry%22%7D%5D%2C%22abstractNote%22%3A%22Enhanced%20vertical%20carbon%20transport%20%28gravitational%20sinking%20and%20subduction%29%20at%20mesoscale%20ocean%20fronts%20may%20explain%20the%20demonstrated%20imbalance%20of%20new%20production%20and%20sinking%20particle%20export%20in%20coastal%20upwelling%20ecosystems.%20Based%20on%20flux%20assessments%20from%20U-238%3ATh-234%20disequilibrium%20and%20sediment%20traps%2C%20we%20found%202%20to%203%20times%20higher%20rates%20of%20gravitational%20particle%20export%20near%20a%20deep-water%20front%20%28305%20mg%20C.m%28-2%29.d%28-1%29%29%20compared%20with%20adjacent%20water%20or%20to%20mean%20%28nonfrontal%29%20regional%20conditions.%20Elevated%20particle%20flux%20at%20the%20front%20wasmechanistically%20linked%20to%20Fe-stressed%20diatoms%20and%20high-mesozooplankton%20fecal%20pellet%20production.%20Using%20a%20data%20assimilative%20regional%20ocean%20model%20fit%20to%20measured%20conditions%2C%20we%20estimate%20that%20an%20additional%20similar%20to%20225%20mg%20C.m%28-2%29.d%28-1%29%20was%20exported%20as%20subduction%20of%20particle-rich%20water%20at%20the%20front%2C%20highlighting%20a%20transport%20mechanism%20that%20is%20not%20captured%20by%20sediment%20traps%20and%20is%20poorly%20quantified%20by%20most%20models%20and%20in%20situ%20measurements.%20Mesoscale%20fronts%20may%20be%20responsible%20for%20over%20a%20quarter%20of%20total%20organic%20carbon%20sequestration%20in%20the%20California%20Current%20and%20other%20coastal%20upwelling%20ecosystems.%22%2C%22date%22%3A%222017%5C%2F02%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1073%5C%2Fpnas.1609435114%22%2C%22ISSN%22%3A%220027-8424%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22QIYZ9CQ7%22%2C%22MWYMG4GN%22%2C%22FWE37XSJ%22%2C%22R4DENPGW%22%2C%22WJTCAXQW%22%2C%22PY4MY9R2%22%5D%2C%22dateModified%22%3A%222023-04-10T22%3A50%3A01Z%22%7D%7D%2C%7B%22key%22%3A%22E7MHZTC7%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hogle%20et%20al.%22%2C%22parsedDate%22%3A%222017-01%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHogle%2C%20S.%20L.%2C%20Brahamsha%2C%20B.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282017%29.%20Direct%20heme%20uptake%20by%20phytoplankton-associated%20Roseobacter%20bacteria.%20%3Ci%3EMSystems%3C%5C%2Fi%3E%2C%20%3Ci%3E2%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FmSystems.00124-16%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FmSystems.00124-16%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Direct%20heme%20uptake%20by%20phytoplankton-associated%20Roseobacter%20bacteria%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shane%20L.%22%2C%22lastName%22%3A%22Hogle%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bianca%22%2C%22lastName%22%3A%22Brahamsha%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katherine%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Joshua%22%2C%22lastName%22%3A%22Weitz%22%7D%5D%2C%22abstractNote%22%3A%22Iron%20is%20an%20essential%20micronutrient%20and%20can%20limit%20the%20growth%20of%20both%20marine%20phytoplankton%20and%20heterotrophic%20bacterioplankton.%20In%20this%20study%2C%20we%20investigated%20the%20molecular%20basis%20of%20heme%20transport%2C%20an%20organic%20iron%20acquisition%20pathway%2C%20in%20phytoplankton-associated%20Roseobacter%20bacteria%20and%20explored%20the%20potential%20role%20of%20bacterial%20heme%20uptake%20in%20the%20marine%20environment.%20We%20searched%20153%20Roseobacter%20genomes%20and%20found%20that%20nearly%20half%20contained%20putative%20complete%20heme%20transport%20systems%20with%20nearly%20the%20same%20synteny.%20We%20also%20examined%20a%20publicly%20available%20coculture%20transcriptome%20and%20found%20that%20Roseobacter%20strain%20Sulfitobacter%20sp.%20strain%20SA11%20strongly%20downregulated%20a%20putative%20heme%20transport%20gene%20cluster%20during%20mutualistic%20growth%20with%20a%20marine%20diatom%2C%20suggesting%20that%20the%20regulation%20of%20heme%20transport%20might%20be%20influenced%20by%20host%20cues.%20We%20generated%20a%20mutant%20of%20phytoplankton-associated%20Roseobacter%20strain%20Ruegeria%20sp.%20strain%20TM1040%20by%20insertionally%20inactivating%20its%20homolog%20of%20the%20TonB-dependent%20heme%20transporter%20hmuR%20and%20confirmed%20the%20role%20of%20this%20gene%20in%20the%20uptake%20of%20heme%20and%20hemoproteins.%20We%20performed%20competition%20experiments%20between%20iron-limited%20wild-type%20and%20mutant%20TM1040%20strains%20and%20found%20that%20the%20wild%20type%20maintains%20a%20growth%20advantage%20when%20competing%20with%20the%20mutant%20for%20iron%20compounds%20derived%20solely%20from%20lysed%20diatom%20cells.%20Heme%20transport%20systems%20were%20largely%20absent%20from%20public%20marine%20metagenomes%20and%20metatranscriptomes%2C%20suggesting%20that%20marine%20bacteria%20with%20the%20potential%20for%20heme%20transport%20likely%20have%20small%20standing%20populations%20in%20the%20free-living%20bacterioplankton.%20Heme%20transport%20is%20likely%20a%20useful%20strategy%20for%20phytoplankton-associated%20bacteria%20because%20it%20provides%20direct%20access%20to%20components%20of%20the%20host%20intracellular%20iron%20pool%20after%20lysis.IMPORTANCE%20Ecosystem%20productivity%20in%20large%20regions%20of%20the%20surface%20ocean%20is%20fueled%20by%20iron%20that%20has%20been%20microbially%20regenerated%20from%20biomass.%20Currently%2C%20the%20specific%20microbes%20and%20molecules%20that%20mediate%20the%20transfer%20of%20recycled%20iron%20between%20microbial%20trophic%20levels%20remain%20largely%20unknown.%20We%20characterized%20a%20marine%20bacterial%20heme%20transporter%20and%20verified%20its%20role%20in%20acquiring%20heme%2C%20an%20abundant%20iron-containing%20enzyme%20cofactor.%20We%20present%20evidence%20that%20after%20host%20cell%20lysis%2C%20phytoplankton-associated%20bacteria%20directly%20extract%20heme%20and%20hemoproteins%20from%20algal%20cellular%20debris%20in%20order%20to%20fulfill%20their%20iron%20requirements%20and%20that%20the%20regulation%20of%20this%20process%20may%20be%20modulated%20by%20host%20cues.%20Direct%20heme%20transport%2C%20in%20contrast%20to%20multistep%20extracellular%20processing%20of%20hemoproteins%2C%20may%20allow%20certain%20phytoplankton-associated%20bacteria%20to%20rapidly%20extract%20iron%20from%20decaying%20phytoplankton%2C%20thus%20efficiently%20recycling%20cellular%20iron%20into%20the%20wider%20microbial%20loop.%22%2C%22date%22%3A%222017%5C%2F01%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1128%5C%2FmSystems.00124-16%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%2C%222XNW8SB5%22%5D%2C%22dateModified%22%3A%222023-04-10T22%3A52%3A17Z%22%7D%7D%2C%7B%22key%22%3A%22M9P5KRFF%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Boiteau%20et%20al.%22%2C%22parsedDate%22%3A%222016-11%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBoiteau%2C%20R.%20M.%2C%20Till%2C%20C.%20P.%2C%20Ruacho%2C%20A.%2C%20Bundy%2C%20R.%20M.%2C%20Hawco%2C%20N.%20J.%2C%20McKenna%2C%20A.%20M.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20Bruland%2C%20K.%20W.%2C%20Saito%2C%20M.%20A.%2C%20%26amp%3B%20Repeta%2C%20D.%20J.%20%282016%29.%20Structural%20characterization%20of%20natural%20nickel%20and%20copper%20binding%20ligands%20along%20the%20US%20GEOTRACES%20Eastern%20Pacific%20Zonal%20Transect.%20%3Ci%3EFrontiers%20in%20Marine%20Science%3C%5C%2Fi%3E%2C%20%3Ci%3E3%3C%5C%2Fi%3E%28243%29.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmars.2016.00243%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmars.2016.00243%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Structural%20characterization%20of%20natural%20nickel%20and%20copper%20binding%20ligands%20along%20the%20US%20GEOTRACES%20Eastern%20Pacific%20Zonal%20Transect%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rene%20M.%22%2C%22lastName%22%3A%22Boiteau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claire%20P.%22%2C%22lastName%22%3A%22Till%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Angel%22%2C%22lastName%22%3A%22Ruacho%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Randelle%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicholas%20J.%22%2C%22lastName%22%3A%22Hawco%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Amy%20M.%22%2C%22lastName%22%3A%22McKenna%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katherine%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kenneth%20W.%22%2C%22lastName%22%3A%22Bruland%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mak%20A.%22%2C%22lastName%22%3A%22Saito%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniel%20J.%22%2C%22lastName%22%3A%22Repeta%22%7D%5D%2C%22abstractNote%22%3A%22Organic%20ligands%20form%20strong%20complexes%20with%20many%20trace%20elements%20in%20seawater.%20Various%20metals%20can%20compete%20for%20the%20same%20ligand%20chelation%20sites%2C%20and%20the%20final%20speciation%20of%20bound%20metals%20is%20determined%20by%20relative%20binding%20affinities%2C%20concentrations%20of%20binding%20sites%2C%20uncomplexed%20metal%20concentrations%2C%20and%20association%5C%2Fdissociation%20kinetics.%20Different%20ligands%20have%20a%20wide%20range%20of%20metal%20affinities%20and%20specificities.%20However%2C%20the%20chemical%20composition%20of%20these%20ligands%20in%20the%20marine%20environment%20remains%20poorly%20constrained%2C%20which%20has%20hindered%20progress%20in%20modeling%20marine%20metal%20speciation.%20In%20this%20study%2C%20we%20detected%20and%20characterized%20natural%20ligands%20that%20bind%20copper%20%28Cu%29%20and%20nickel%20%28Ni%29%20in%20the%20eastern%20South%20Pacific%20Ocean%20with%20liquid%20chromatography%20tandem%20inductively%20coupled%20plasma%20mass%20spectrometry%20%28LC-ICPMS%29%2C%20and%20high%20resolution%20electrospray%20ionization%20mass%20spectrometry%20%28ESIMS%29.%20Dissolved%20Cu%2C%20Ni%2C%20and%20ligand%20concentrations%20were%20highest%20near%20the%20coast.%20Chromatographically%20unresolved%20polar%20compounds%20dominated%20ligands%20isolated%20near%20the%20coast%20by%20solid%20phase%20extraction.%20Offshore%2C%20metal%20and%20ligand%20concentrations%20decreased%2C%20but%20several%20new%20ligands%20appeared.%20One%20major%20ligand%20was%20detected%20that%20bound%20both%20Cu2%2B%20and%20Ni2%2B.%20Based%20on%20accurate%20mass%20and%20fragmentation%20measurements%2C%20this%20compound%20has%20a%20molecular%20formula%20of%20%5BC20H21N4O8S2%20%2B%20M%5D%2B%20%28M%20%3D%20metal%20isotope%29%20and%20contains%20several%20azole-like%20metal%20binding%20groups.%20Additional%20lipophilic%20Ni%20complexes%20were%20also%20present%20only%20in%20oligotrophic%20waters%2C%20with%20masses%20of%20649%2C%20698%2C%20and%20712%20m%5C%2Fz%20%28corresponding%20to%20the%2058Ni%20metal%20complex%29.%20Molecular%20formulae%20of%20%5BC32H54N3O6S2Ni%5D%2B%20and%20%5BC33H56N3O6S2Ni%5D%2B%20were%20determined%20for%20two%20of%20these%20compounds.%20Addition%20of%20Cu%20and%20Ni%20to%20the%20samples%20also%20revealed%20the%20presence%20of%20additional%20compounds%20that%20can%20bind%20both%20Ni%20and%20Cu.%20Although%20these%20specific%20compounds%20represent%20a%20small%20fraction%20of%20the%20total%20dissolved%20Cu%20and%20Ni%20pool%2C%20they%20highlight%20the%20compositional%20diversity%20and%20spatial%20heterogeneity%20of%20marine%20Ni%20and%20Cu%20ligands%2C%20as%20well%20as%20variability%20in%20the%20extent%20to%20which%20different%20metals%20in%20the%20same%20environment%20compete%20for%20ligand%20binding.%22%2C%22date%22%3A%222016%5C%2F11%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.3389%5C%2Ffmars.2016.00243%22%2C%22ISSN%22%3A%222296-7745%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A17Z%22%7D%7D%2C%7B%22key%22%3A%22MVLRICXI%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hogle%20et%20al.%22%2C%22parsedDate%22%3A%222016-10%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHogle%2C%20S.%20L.%2C%20Bundy%2C%20R.%20M.%2C%20Blanton%2C%20J.%20M.%2C%20Allen%2C%20E.%20E.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282016%29.%20Copiotrophic%20marine%20bacteria%20are%20associated%20with%20strong%20iron-binding%20ligand%20production%20during%20phytoplankton%20blooms.%20%3Ci%3ELimnology%20and%20Oceanography%20Letters%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Flol2.10026%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Flol2.10026%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Copiotrophic%20marine%20bacteria%20are%20associated%20with%20strong%20iron-binding%20ligand%20production%20during%20phytoplankton%20blooms%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shane%20L.%22%2C%22lastName%22%3A%22Hogle%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Randelle%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%20M.%22%2C%22lastName%22%3A%22Blanton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katherine%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22Although%20marine%20bacteria%20were%20identified%20nearly%20two%20decades%20ago%20as%20potential%20sources%20for%20strong%20iron-binding%20organic%20ligands%20detected%20in%20seawater%2C%20specific%20linkages%20between%20ligands%20detected%20in%20natural%20water%20and%20the%20microbial%20community%20remain%20unclear.%20We%20compared%20the%20production%20of%20different%20classes%20of%20iron-binding%20ligands%2C%20dissolved%20iron%20and%20macronutrient%20concentrations%2C%20and%20phytoplankton%20and%20bacterioplankton%20assemblages%20in%20a%20series%20of%20iron%20amended%206-d%20incubations.%20Incubations%20with%20high%20iron%20additions%20had%20near%20complete%20macronutrient%20consumption%20and%20higher%20phytoplankton%20biomass%20compared%20with%20incubations%20with%20low%20iron%20additions%2C%20but%20both%20iron%20treatments%20were%20dominated%20by%20diatoms.%20However%2C%20we%20only%20detected%20the%20strongest%20ligands%20in%20high-iron%20treatments%2C%20and%20strong%20iron-binding%20ligands%20were%20generally%20correlated%20with%20an%20increased%20abundance%20of%20copiotrophic%20bacteria%2C%20particularly%20Alteromonas%20strains.%20Ultimately%2C%20these%20robust%20correlations%20suggest%20a%20potential%20linkage%20between%20copiotrophic%20bacteria%20and%20strong%20iron-binding%20ligand%20production%20after%20iron%20fertilization%20events%20in%20the%20marine%20environment.%22%2C%22date%22%3A%222016%5C%2F10%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1002%5C%2Flol2.10026%22%2C%22ISSN%22%3A%222378-2242%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R4QPIR6K%22%2C%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222023-05-03T20%3A39%3A33Z%22%7D%7D%2C%7B%22key%22%3A%22CCPNR5AQ%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Semeniuk%20et%20al.%22%2C%22parsedDate%22%3A%222016-06%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESemeniuk%2C%20D.%20M.%2C%20Bundy%2C%20R.%20M.%2C%20Posacka%2C%20A.%20M.%2C%20Robert%2C%20M.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20%26amp%3B%20Maldonado%2C%20M.%20T.%20%282016%29.%20Using%2067Cu%20to%20study%20the%20biogeochemical%20cycling%20of%20copper%20in%20the%20northeast%20subarctic%20Pacific%20Ocean.%20%3Ci%3EFrontiers%20in%20Marine%20Science%3C%5C%2Fi%3E%2C%20%3Ci%3E3%3C%5C%2Fi%3E%2878%29.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmars.2016.00078%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmars.2016.00078%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Using%2067Cu%20to%20study%20the%20biogeochemical%20cycling%20of%20copper%20in%20the%20northeast%20subarctic%20Pacific%20Ocean%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%20M.%22%2C%22lastName%22%3A%22Semeniuk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Randelle%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anna%20M.%22%2C%22lastName%22%3A%22Posacka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie%22%2C%22lastName%22%3A%22Robert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katherine%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%20T.%22%2C%22lastName%22%3A%22Maldonado%22%7D%5D%2C%22abstractNote%22%3A%22Microbial%20copper%20%28Cu%29%20nutrition%20and%20dissolved%20Cu%20speciation%20were%20surveyed%20along%20Line%20P%2C%20a%20coastal%20to%20open%20ocean%20transect%20that%20extends%20from%20the%20coast%20of%20British%20Columbia%2C%20Canada%2C%20to%20the%20high-nutrient-low-chlorophyll%20%28HNLC%29%20zone%20of%20the%20northeast%20subarctic%20Pacific%20Ocean.%20Steady-state%20size%20fractionated%20Cu%20uptake%20rates%20and%20Cu%3AC%20assimilation%20ratios%20were%20determined%20at%20in%20situ%20Cu%20concentrations%20and%20speciation%20using%20a%2067Cu%20tracer%20method.%20The%20cellular%20Cu%3AC%20ratios%20that%20we%20measured%20%28~30%20%5Cu00b5mol%20Cu%20mol%20C-1%29%20are%20similar%20to%20recent%20estimates%20using%20synchrotron%20x-ray%20fluorescence%20%28SXRF%29%2C%20suggesting%20that%20the%2067Cu%20method%20can%20determine%20in%20situ%20metabolic%20Cu%20demands.%20We%20examined%20how%20environmental%20changes%20along%20the%20Line%20P%20transect%20influenced%20Cu%20metabolism%20in%20the%20sub-microplankton%20community.%20Cellular%20Cu%3AC%20assimilation%20ratios%20and%20uptake%20rates%20were%20compared%20with%20net%20primary%20productivity%2C%20bacterial%20abundance%20and%20productivity%2C%20total%20dissolved%20Cu%2C%20Cu%20speciation%2C%20and%20a%20suite%20of%20other%20chemical%20and%20biological%20parameters.%20Total%20dissolved%20Cu%20concentrations%20%28%5BCu%5Dd%29%20were%20within%20a%20narrow%20range%20%281.46%20to%202.79%20nM%29%2C%20and%20Cu%20was%20bound%20to%20a%20~5-fold%20excess%20of%20strong%20ligands%20with%20conditional%20stability%20constants%20%28%20%29%20of%20~1014.%20Free%20Cu2%2B%20concentrations%20were%20low%20%28pCu%2014.4%20to%2015.1%29%2C%20and%20total%20and%20size%20fractionated%20net%20primary%20productivity%20%28NPPV%3B%20%5Cu00b5g%20C%20L-1%20d-1%29%20were%20negatively%20correlated%20with%20inorganic%20Cu%20concentrations%20%28%5BCu%5Cu2032%5D%29.%20We%20suggest%20this%20is%20due%20to%20greater%20Cu%5Cu2032%20drawdown%20by%20faster%20growing%20phytoplankton%20populations.%20Using%20the%20relationship%20between%20%5BCu%5Cu2032%5D%20drawdown%20and%20NPPV%2C%20we%20calculated%20a%20regional%20photosynthetic%20Cu%3AC%20drawdown%20export%20ratio%20between%201.5%20and%2015%20%5Cu00b5mol%20Cu%20mol%20C-1%2C%20and%20a%20mixed%20layer%20residence%20time%20%282.5%20to%208%20years%29%20that%20is%20similar%20to%20other%20independent%20estimates%20%282-12%20years%29.%20Total%20particulate%20Cu%20uptake%20rates%20were%20between%2022%20and%20125%20times%20faster%20than%20estimates%20of%20Cu%20export%3B%20this%20is%20possibly%20mediated%20by%20rapid%20cellular%20Cu%20uptake%20and%20efflux%20by%20phytoplankton%20and%20bacteria%20or%20the%20effects%20of%20grazers%20and%20bacterial%20remineralization%20on%20dissolved%20Cu.%20These%20results%20provide%20a%20more%20detailed%20understanding%20of%20the%20interactions%20between%20Cu%20speciation%20and%20microorganisms%20in%20seawater%2C%20and%20present%20evidence%20that%20marine%20phytoplankton%20modify%20Cu%20speciation%20in%20the%20open%20ocean.%22%2C%22date%22%3A%222016%5C%2F06%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.3389%5C%2Ffmars.2016.00078%22%2C%22ISSN%22%3A%222296-7745%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A17Z%22%7D%7D%2C%7B%22key%22%3A%226KXJYVNG%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hogle%20et%20al.%22%2C%22parsedDate%22%3A%222016-03%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHogle%2C%20S.%20L.%2C%20Thrash%2C%20J.%20C.%2C%20Dupont%2C%20C.%20L.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282016%29.%20Trace%20metal%20acquisition%20by%20marine%20heterotrophic%20bacterioplankton%20with%20contrasting%20trophic%20strategies.%20%3Ci%3EApplied%20and%20Environmental%20Microbiology%3C%5C%2Fi%3E%2C%20%3Ci%3E82%3C%5C%2Fi%3E%285%29%2C%201613%26%23x2013%3B1624.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Faem.03128-15%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Faem.03128-15%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Trace%20metal%20acquisition%20by%20marine%20heterotrophic%20bacterioplankton%20with%20contrasting%20trophic%20strategies%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%20L.%22%2C%22lastName%22%3A%22Hogle%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20C.%22%2C%22lastName%22%3A%22Thrash%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20L.%22%2C%22lastName%22%3A%22Dupont%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22Heterotrophic%20bacteria%20in%20the%20SAR11%20and%20Roseobacter%20lineages%20shape%20the%20marine%20carbon%2C%20nitrogen%2C%20phosphorous%2C%20and%20sulfur%20cycles%2C%20yet%20they%20do%20so%20having%20adopted%20divergent%20ecological%20strategies.%20Currently%2C%20it%20is%20unknown%20whether%20these%20globally%20significant%20groups%20partition%20into%20specific%20niches%20with%20respect%20to%20micronutrients%20%28e.g.%2C%20trace%20metals%29%20and%20how%20that%20may%20affect%20marine%20trace%20metal%20cycling.%20Here%2C%20we%20used%20comparative%20genomics%20to%20identify%20diverse%20iron%2C%20cobalt%2C%20nickel%2C%20copper%2C%20and%20zinc%20uptake%20capabilities%20in%20SAR11%20and%20Roseobacter%20genomes%20and%20uncover%20surprising%20unevenness%20within%20and%20between%20lineages.%20The%20strongest%20predictors%20for%20the%20extent%20of%20the%20metal%20uptake%20gene%20content%20are%20the%20total%20number%20of%20transporters%20per%20genome%2C%20genome%20size%2C%20total%20metal%20transporters%2C%20and%20GC%20content%2C%20but%20numerous%20exceptions%20exist%20in%20both%20groups.%20Taken%20together%2C%20our%20results%20suggest%20that%20SAR11%20have%20strongly%20minimized%20their%20trace%20metal%20uptake%20versatility%2C%20with%20high-affinity%20zinc%20uptake%20being%20a%20unique%20exception.%20The%20larger%20Roseobacter%20genomes%20have%20greater%20trace%20metal%20uptake%20versatility%20on%20average%2C%20but%20they%20also%20appear%20to%20have%20greater%20plasticity%2C%20resulting%20in%20phylogenetically%20similar%20genomes%20having%20largely%20different%20capabilities.%20Ultimately%2C%20phylogeny%20is%20predictive%20of%20the%20diversity%20and%20extent%20of%2020%20to%2033%25%20of%20all%20metal%20uptake%20systems%2C%20suggesting%20that%20specialization%20in%20metal%20utilization%20mostly%20occurred%20independently%20from%20overall%20lineage%20diversification%20in%20both%20SAR11%20and%20Roseobacter.%20We%20interpret%20these%20results%20as%20reflecting%20relatively%20recent%20trace%20metal%20niche%20partitioning%20in%20both%20lineages%2C%20suggesting%20that%20concentrations%20and%20chemical%20forms%20of%20metals%20in%20the%20marine%20environment%20are%20important%20factors%20shaping%20the%20gene%20content%20of%20marine%20heterotrophic%20Alphaproteobacteria%20of%20the%20SAR11%20and%20Roseobacter%20lineages.%22%2C%22date%22%3A%222016%5C%2F03%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1128%5C%2Faem.03128-15%22%2C%22ISSN%22%3A%220099-2240%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A17Z%22%7D%7D%2C%7B%22key%22%3A%22336UUNN3%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Bundy%20et%20al.%22%2C%22parsedDate%22%3A%222016-03%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBundy%2C%20R.%20M.%2C%20Jiang%2C%20M.%2C%20Carter%2C%20M.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282016%29.%20Iron-binding%20ligands%20in%20the%20southern%20California%20Current%20System%3A%20Mechanistic%20studies.%20%3Ci%3EFrontiers%20in%20Marine%20Science%3C%5C%2Fi%3E%2C%20%3Ci%3E3%3C%5C%2Fi%3E%2827%29.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmars.2016.00027%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmars.2016.00027%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Iron-binding%20ligands%20in%20the%20southern%20California%20Current%20System%3A%20Mechanistic%20studies%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Randelle%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mingshun%22%2C%22lastName%22%3A%22Jiang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Melissa%22%2C%22lastName%22%3A%22Carter%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katherine%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22The%20distributions%20of%20dissolved%20iron%20and%20organic%20iron-binding%20ligands%20were%20examined%20in%20water%20column%20profiles%20and%20deckboard%20incubation%20experiments%20in%20the%20southern%20California%20Current%20System%20%28sCCS%29%20along%20a%20transition%20from%20coastal%20to%20semi-oligotrophic%20waters.%20Analysis%20of%20the%20iron-binding%20ligand%20pool%20by%20competitive%20ligand%20exchange-adsorptive%20cathodic%20stripping%20voltammetry%20%28CLE-ACSV%29%20using%20multiple%20analytical%20windows%20%28MAWs%29%20revealed%20three%20classes%20of%20iron-binding%20ligands%20present%20throughout%20the%20water%20column%20%28L1-L3%29%2C%20whose%20distributions%20closely%20matched%20those%20of%20dissolved%20iron%20and%20nitrate.%20Despite%20significant%20biogeochemical%20gradients%2C%20ligand%20profiles%20were%20similar%20between%20stations%2C%20with%20surface%20minima%20in%20strong%20ligands%20%28L1%20and%20L2%29%2C%20and%20relatively%20constant%20concentrations%20of%20weaker%20ligands%20%28L3%29%20down%20to%20500%20m.%20A%20phytoplankton%20grow-out%20incubation%2C%20initiated%20from%20an%20iron-limited%20water%20mass%2C%20showed%20dynamic%20temporal%20cycling%20of%20iron-binding%20ligands.%20A%20biological%20iron%20model%20was%20able%20to%20capture%20the%20patterns%20of%20the%20strong%20ligands%20in%20the%20grow-out%20incubation%20relatively%20well%20with%20only%20the%20microbial%20community%20as%20a%20biological%20source.%20An%20experiment%20focused%20on%20remineralization%20of%20particulate%20organic%20matter%20showed%20production%20of%20both%20strong%20and%20weak%20iron-binding%20ligands%20by%20the%20heterotrophic%20community%2C%20supporting%20a%20mechanism%20for%20in-situ%20production%20of%20both%20strong%20and%20weak%20iron-binding%20ligands%20in%20the%20subsurface%20water%20column.%20Photochemical%20experiments%20showed%20a%20variable%20influence%20of%20sunlight%20on%20the%20degradation%20of%20natural%20iron-binding%20ligands%2C%20providing%20some%20evidence%20to%20explain%20differences%20in%20surface%20ligand%20concentrations%20between%20stations.%20Patterns%20in%20ligand%20distributions%20between%20profiles%20and%20in%20the%20incubation%20experiments%20were%20primarily%20related%20to%20macronutrient%20concentrations%2C%20suggesting%20microbial%20remineralization%20processes%20might%20dominate%20on%20longer%20time-scales%20over%20short-term%20changes%20associated%20with%20photochemistry%20or%20phytoplankton%20growth.%22%2C%22date%22%3A%222016%5C%2F03%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.3389%5C%2Ffmars.2016.00027%22%2C%22ISSN%22%3A%222296-7745%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A15Z%22%7D%7D%2C%7B%22key%22%3A%22YQW6QPY5%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Semeniuk%20et%20al.%22%2C%22parsedDate%22%3A%222016-01%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESemeniuk%2C%20D.%20M.%2C%20Taylor%2C%20R.%20L.%2C%20Bundy%2C%20R.%20M.%2C%20Johnson%2C%20W.%20K.%2C%20Cullen%2C%20J.%20T.%2C%20Robert%2C%20M.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20%26amp%3B%20Maldonado%2C%20M.%20T.%20%282016%29.%20Iron-copper%20interactions%20in%20iron-limited%20phytoplankton%20in%20the%20northeast%20subarctic%20Pacific%20Ocean.%20%3Ci%3ELimnology%20and%20Oceanography%3C%5C%2Fi%3E%2C%20%3Ci%3E61%3C%5C%2Fi%3E%281%29%2C%20279%26%23x2013%3B297.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Flno.10210%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Flno.10210%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Iron-copper%20interactions%20in%20iron-limited%20phytoplankton%20in%20the%20northeast%20subarctic%20Pacific%20Ocean%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%20M.%22%2C%22lastName%22%3A%22Semeniuk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20L.%22%2C%22lastName%22%3A%22Taylor%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22W.%20K.%22%2C%22lastName%22%3A%22Johnson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20T.%22%2C%22lastName%22%3A%22Cullen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Robert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20T.%22%2C%22lastName%22%3A%22Maldonado%22%7D%5D%2C%22abstractNote%22%3A%22In%20August%202010%2C%20iron%20%28Fe%29%20and%20Fe%20and%20copper%20%28Cu%29%20addition%20incubation%20experiments%20were%20conducted%20at%20two%20low%20Fe%20stations%20%28P20%20and%20P26%29%20along%20Line%20P%2C%20off%20the%20western%20coast%20of%20British%20Columbia%2C%20to%20investigate%20Cu%20physiology%20in%20Fe-%20and%20Fe-light%20co-limited%20phytoplankton.%20Chlorophyll%20a%20concentrations%20%28%5BChl%20a%5D%29%2C%20maximum%20variable%20fluorescence%20yield%20%28F-v%5C%2FF-m%29%2C%20and%20Fe%20uptake%20rates%20by%20the%20Cu-dependent%20high-affinity%20Fe%20transport%20system%20%28HAFeTS%29%20were%20measured.%20Additions%20of%20Fe%20resulted%20in%20an%20increase%20in%20%5BChl%20a%5D%20and%20F-v%5C%2FF-m%20at%20both%20stations%20compared%20with%20the%20controls%2C%20regardless%20of%20light%20availability%2C%20and%20confirmed%20that%20the%20phytoplankton%20communities%20were%20Fe-limited.%20Uptake%20of%20Fe%20by%20the%20HAFeTS%20in%20both%20incubations%20increased%20with%20the%20addition%20of%20Fe%2C%20and%20likely%20reflects%20luxury%20Fe%20uptake%20and%20storage.%20While%20the%20in%20situ%20inorganic%20Cu%20concentrations%20were%20similar%20to%20those%20that%20can%20induce%20Cu-limitation%20in%20laboratory%20cultures%2C%20increasing%20Cu%20availability%20had%20no%20effect%20on%20biomass%20accumulation%20during%20both%20incubations%2C%20regardless%20of%20Fe%20availability%20or%20light%20regime.%20At%20P26%2C%20additions%20of%201%20nmol%20L-1%20CuSO4%20resulted%20in%20a%20short-term%20increase%20in%20F-v%5C%2FF-m%20of%20the%20phytoplankton%20community%2C%20and%20an%20increase%20in%20Fe%20uptake%20rates%20by%20large%20phytoplankton%20%28%3E5%20mu%20m%29%2C%20but%20only%20when%20light%20was%20not%20limiting.%20These%20data%20confirm%20a%20complex%20interaction%20between%20light%2C%20Fe%20and%20Cu%20physiology%20in%20indigenous%20phytoplankton%20communities%2C%20and%20suggest%20that%20these%20interactions%20may%20be%20both%20spatially%20heterogeneous%20and%20different%20for%20different%20phytoplankton%20size%20classes.%22%2C%22date%22%3A%222016%5C%2F01%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1002%5C%2Flno.10210%22%2C%22ISSN%22%3A%220024-3590%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A15Z%22%7D%7D%2C%7B%22key%22%3A%229H9CB4DU%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Brzezinski%20et%20al.%22%2C%22parsedDate%22%3A%222015-07%22%2C%22numChildren%22%3A8%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBrzezinski%2C%20M.%20A.%2C%20Krause%2C%20J.%20W.%2C%20Bundy%2C%20R.%20M.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20Franks%2C%20P.%2C%20Goericke%2C%20R.%2C%20Landry%2C%20M.%20R.%2C%20%26amp%3B%20Stukel%2C%20M.%20R.%20%282015%29.%20Enhanced%20silica%20ballasting%20from%20iron%20stress%20sustains%20carbon%20export%20in%20a%20frontal%20zone%20within%20the%20California%20Current.%20%3Ci%3EJournal%20of%20Geophysical%20Research-Oceans%3C%5C%2Fi%3E%2C%20%3Ci%3E120%3C%5C%2Fi%3E%287%29%2C%204654%26%23x2013%3B4669.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2F2015jc010829%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2F2015jc010829%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Enhanced%20silica%20ballasting%20from%20iron%20stress%20sustains%20carbon%20export%20in%20a%20frontal%20zone%20within%20the%20California%20Current%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20A.%22%2C%22lastName%22%3A%22Brzezinski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20W.%22%2C%22lastName%22%3A%22Krause%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Franks%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Goericke%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20R.%22%2C%22lastName%22%3A%22Landry%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20R.%22%2C%22lastName%22%3A%22Stukel%22%7D%5D%2C%22abstractNote%22%3A%22Nutrient%20dynamics%2C%20phytoplankton%20rate%20processes%2C%20and%20export%20were%20examined%20in%20a%20frontal%20region%20between%20an%20anticyclone%20and%20a%20pair%20of%20cyclones%20120%20km%20off%20the%20coast%20in%20the%20southern%20California%20Current%20System%20%28sCCS%29.%20Low%20silicic%20acid%3A%20nitrate%20ratios%20%28Si%3AN%29%20and%20high%20nitrate%20to%20iron%20ratios%20%28N%3A%20Fe%29%20characteristic%20of%20Fe-limiting%20conditions%20in%20the%20sCCS%20were%20associated%20with%20the%20northern%20cyclone%20and%20with%20the%20transition%20zone%20between%20the%20cyclones%20and%20the%20anticyclone.%20Phytoplankton%20growth%20in%20low-Si%3AN%2C%20high-N%3AFe%20waters%20responded%20strongly%20to%20added%20Fe%2C%20confirming%20growth%20limitation%20by%20Fe%20of%20the%20diatom-dominated%20phytoplankton%20community.%20Low%20Si%3A%20N%20waters%20had%20low%20biogenic%20silica%20content%2C%20intermediate%20productivity%2C%20but%20high%20export%20compared%20to%20intermediate%20Si%3A%20N%20waters%20indicating%20increased%20export%20efficiency%20under%20Fe%20stress.%20Biogenic%20silica%20and%20particulate%20organic%20carbon%20%28POC%29%20export%20were%20both%20high%20beneath%20low%20Si%3A%20N%20waters%20with%20biogenic%20silica%20export%20being%20especially%20enhanced.%20This%20suggests%20that%20relatively%20high%20POC%20export%20from%20low%20Si%3A%20N%20waters%20was%20supported%20by%20silica%20ballasting%20from%20Fe-limited%20diatoms.%20Higher%20POC%20export%20efficiency%20in%20low%20Si%3A%20N%20waters%20may%20have%20been%20further%20enhanced%20by%20lower%20rates%20of%20organic%20carbon%20remineralization%20due%20to%20reduced%20grazing%20of%20more%20heavily%20armored%20diatoms%20growing%20under%20Fe%20stress.%20The%20results%20imply%20that%20Fe%20stress%20can%20enhance%20carbon%20export%2C%20despite%20lowering%20productivity%2C%20by%20driving%20higher%20export%20efficiency.%22%2C%22date%22%3A%222015%5C%2F07%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1002%5C%2F2015jc010829%22%2C%22ISSN%22%3A%222169-9275%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%2C%22BZBPGKQB%22%2C%22FWE37XSJ%22%2C%22PY4MY9R2%22%5D%2C%22dateModified%22%3A%222024-04-12T20%3A13%3A42Z%22%7D%7D%2C%7B%22key%22%3A%228NF4LZQ8%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Bundy%20et%20al.%22%2C%22parsedDate%22%3A%222015-07%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBundy%2C%20R.%20M.%2C%20Abdulla%2C%20H.%20A.%20N.%2C%20Hatcher%2C%20P.%20G.%2C%20Biller%2C%20D.%20V.%2C%20Buck%2C%20K.%20N.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282015%29.%20Iron-binding%20ligands%20and%20humic%20substances%20in%20the%20San%20Francisco%20Bay%20estuary%20and%20estuarine-influenced%20shelf%20regions%20of%20coastal%20California.%20%3Ci%3EMarine%20Chemistry%3C%5C%2Fi%3E%2C%20%3Ci%3E173%3C%5C%2Fi%3E%2C%20183%26%23x2013%3B194.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2014.11.005%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2014.11.005%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Iron-binding%20ligands%20and%20humic%20substances%20in%20the%20San%20Francisco%20Bay%20estuary%20and%20estuarine-influenced%20shelf%20regions%20of%20coastal%20California%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22H.%20A.%20N.%22%2C%22lastName%22%3A%22Abdulla%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%20G.%22%2C%22lastName%22%3A%22Hatcher%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%20V.%22%2C%22lastName%22%3A%22Biller%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20N.%22%2C%22lastName%22%3A%22Buck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22Dissolved%20iron%20%28dFe%29%20and%20organic%20dFe-binding%20ligands%20were%20determined%20in%20San%20Francisco%20Bay%2C%20California%20by%20competitive%20ligand%20exchange%20adsorptive%20cathodic%20stripping%20voltammetry%20%28CLE-ACSV%29%20along%20a%20salinity%20gradient%20from%20the%20freshwater%20endmember%20of%20the%20Sacramento%20River%20%28salinity%20%3C2%29%20to%20the%20mouth%20of%20the%20estuary%20%28salinity%20%3E26%29.%20A%20range%20of%20dFe-binding%20ligand%20classes%20was%20simultaneously%20determined%20using%20multiple%20analytical%20window%20analysis%2C%20involving%20titrations%20with%20multiple%20concentrations%20of%20the%20added%20ligand%2Csalicylaldoxime.%20The%20highest%20dFe%20and%20ligand%20concentrations%20were%20determined%20in%20the%20low%20salinity%20end%20of%20the%20estuary%2C%20with%20dFe%20equal%20to%20131.5%20nmol%20L-1%20and%20strong%20ligand%20%28log%20K-Fel%2C%20Fe%27%28cond%29%20%3E%3D%2012.0%29%20concentrations%20equal%20to%20139.5%20nmol%20L-1.%20The%20weakest%20ligands%20%28log%20K-Fel%2C%20Fe%27%28cond%29%20%3C%2010.0%29%20were%20always%20in%20excess%20of%20dFe%20in%20low%20salinity%20waters%2C%20but%20were%20rapidly%20flocculated%20within%20the%20estuary%20and%20were%20not%20detected%20at%20salinities%20greater%20than%207.%20The%20strongest%20ligands%20%28log%20K-Fel%2C%20Fe%27%28cond%29%20%3E%2011.0%29%20were%20tightly%20coupled%20to%20dFe%20throughout%20the%20estuary%2C%20with%20average%20excess%20ligand%20concentrations%20%28%5BL%5D-%5BdFe%5D%29%20equal%20to%200.5%20nmol%20L-1.%20Humic-like%20substances%20analyzed%20via%20both%20CLE-ACSV%20and%20proton%20nuclear%20magnetic%20resonance%20in%20several%20samples%20were%20found%20to%20be%20a%20significant%20portion%20of%20the%20dFe-binding%20ligand%20pool%20in%20San%20Francisco%20Bay%2C%20with%20concentrations%20ranging%20from%20559.5%20mu%20g%20L-1%20to%2067.5%20mu%20g%20L-1%20in%20the%20lowest%20and%20highest%20salinity%20samples%2C%20respectively.%20DFe-binding%20ligands%20and%20humic-like%20substances%20were%20also%20found%20in%20benthic%20boundary%20layer%20samples%20taken%20from%20the%20shelf%20near%20the%20mouths%20of%20San%20Francisco%20Bay%20and%20Eel%20River%2C%20suggesting%20estuaries%20are%20an%20important%20source%20of%20dFe-binding%20ligands%20to%20California%20coastal%20shelf%20waters.%20%28C%29%202014%20Elsevier%20B.V.%20All%20rights%20reserved.%22%2C%22date%22%3A%222015%5C%2F07%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.marchem.2014.11.005%22%2C%22ISSN%22%3A%220304-4203%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A15Z%22%7D%7D%2C%7B%22key%22%3A%22EGGSBIXY%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Pizeta%20et%20al.%22%2C%22parsedDate%22%3A%222015-07%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPizeta%2C%20I.%2C%20Sander%2C%20S.%20G.%2C%20Hudson%2C%20R.%20J.%20M.%2C%20Omanovic%2C%20D.%2C%20Baars%2C%20O.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20Buck%2C%20K.%20N.%2C%20Bundy%2C%20R.%20M.%2C%20Carrasco%2C%20G.%2C%20Croot%2C%20P.%20L.%2C%20Garnier%2C%20C.%2C%20Gerringa%2C%20L.%20J.%20A.%2C%20Gledhill%2C%20M.%2C%20Hirose%2C%20K.%2C%20Kondo%2C%20Y.%2C%20Laglera%2C%20L.%20M.%2C%20Nuester%2C%20J.%2C%20Rijkenberg%2C%20M.%20J.%20A.%2C%20Takeda%2C%20S.%2C%20%26%23x2026%3B%20Wells%2C%20M.%20%282015%29.%20Interpretation%20of%20complexometric%20titration%20data%3A%20An%20intercomparison%20of%20methods%20for%20estimating%20models%20of%20trace%20metal%20complexation%20by%20natural%20organic%20ligands.%20%3Ci%3EMarine%20Chemistry%3C%5C%2Fi%3E%2C%20%3Ci%3E173%3C%5C%2Fi%3E%2C%203%26%23x2013%3B24.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2015.03.006%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2015.03.006%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Interpretation%20of%20complexometric%20titration%20data%3A%20An%20intercomparison%20of%20methods%20for%20estimating%20models%20of%20trace%20metal%20complexation%20by%20natural%20organic%20ligands%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22I.%22%2C%22lastName%22%3A%22Pizeta%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%20G.%22%2C%22lastName%22%3A%22Sander%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20J.%20M.%22%2C%22lastName%22%3A%22Hudson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%22%2C%22lastName%22%3A%22Omanovic%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22O.%22%2C%22lastName%22%3A%22Baars%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20N.%22%2C%22lastName%22%3A%22Buck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22G.%22%2C%22lastName%22%3A%22Carrasco%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%20L.%22%2C%22lastName%22%3A%22Croot%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%22%2C%22lastName%22%3A%22Garnier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%20J.%20A.%22%2C%22lastName%22%3A%22Gerringa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Gledhill%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Hirose%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Y.%22%2C%22lastName%22%3A%22Kondo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22L.%20M.%22%2C%22lastName%22%3A%22Laglera%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Nuester%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20J.%20A.%22%2C%22lastName%22%3A%22Rijkenberg%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Takeda%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20S.%22%2C%22lastName%22%3A%22Twining%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Wells%22%7D%5D%2C%22abstractNote%22%3A%22With%20the%20common%20goal%20of%20more%20accurately%20and%20consistently%20quantifying%20ambient%20concentrations%20of%20free%20metal%20ions%20and%20natural%20organic%20ligands%20in%20aquatic%20ecosystems%2C%20researchers%20from%2015%20laboratories%20that%20routinely%20analyze%20trace%20metal%20speciation%20participated%20in%20an%20intercomparison%20of%20statistical%20methods%20used%20to%20model%20their%20most%20common%20type%20of%20experimental%20dataset%2C%20the%20complexometric%20titration.%20All%20were%20asked%20to%20apply%20statistical%20techniques%20that%20they%20were%20familiar%20with%20to%20model%20synthetic%20titration%20data%20that%20are%20typical%20of%20those%20obtained%20by%20applying%20state-of-the-art%20electrochemical%20methods%20-%20anodic%20stripping%20voltammetry%20%28ASV%29%20and%20competitive%20ligand%20equilibration-adsorptive%20cathodic%20stripping%20voltammetry%20%28CLE-ACSV%29%20-%20to%20the%20analysis%20of%20natural%20waters.%20Herein%2C%20we%20compare%20their%20estimates%20for%20parameters%20describing%20the%20natural%20ligands%2C%20examine%20the%20accuracy%20of%20inferred%20ambient%20free%20metal%20ion%20concentrations%20%28%5DM-f%5D%29%2C%20and%20evaluate%20the%20influence%20of%20the%20various%20methods%20and%20assumptions%20used%20on%20these%20results.%20The%20ASV-type%20titrations%20were%20designed%20to%20test%20each%20participant%27s%20ability%20to%20correctly%20describe%20the%20natural%20ligands%20present%20in%20a%20sample%20when%20provided%20with%20data%20free%20of%20measurement%20error%2C%20i.e.%2C%20random%20noise.%20For%20the%20three%20virtual%20samples%20containing%20just%20one%20natural%20ligand%2C%20all%20participants%20were%20able%20to%20correctly%20identify%20the%20number%20of%20ligand%20classes%20present%20and%20accurately%20estimate%20their%20parameters.%20For%20the%20four%20samples%20containing%20two%20or%20three%20ligand%20classes%2C%20a%20few%20participants%20detected%20too%20few%20or%20too%20many%20classes%20and%20consequently%20reported%20inaccurate%20%27measurements%27%20of%20ambient%20%5BM-f%5D.%20Since%20the%20problematic%20results%20arose%20from%20human%20error%20rather%20than%20any%20specific%20method%20of%20analyzing%20the%20data%2C%20we%20recommend%20that%20analysts%20should%20make%20a%20practice%20of%20using%20one%27s%20parameter%20estimates%20to%20generate%20simulated%20%28back-calculated%29%20titration%20curves%20for%20comparison%20to%20the%20original%20data.%20The%20root-mean-squared%20relative%20error%20between%20the%20fitted%20observations%20and%20the%20simulated%20curves%20should%20be%20comparable%20to%20the%20expected%20precision%20of%20the%20analytical%20method%20and%20upon%20visual%20inspection%20the%20distribution%20of%20residuals%20should%20not%20be%20skewed.%20Modeling%20the%20synthetic%2C%20CLE-ACSV-type%20titration%20dataset%2C%20which%20comprises%205%20titration%20curves%20generated%20at%20different%20analytical-windows%20or%20levels%20of%20competing%20ligand%20added%20to%20the%20virtual%20sample%2C%20proved%20to%20be%20more%20challenging%20due%20to%20the%20random%20measurement%20error%20that%20was%20incorporated.%20Comparison%20of%20the%20submitted%20results%20was%20complicated%20by%20the%20participants%27%20differing%20interpretations%20of%20their%20task.%20Most%20adopted%20the%20provided%20%27true%27%20instrumental%20sensitivity%20in%20modeling%20the%20CLE-ACSV%20curves%2C%20but%20several%20estimated%20sensitivities%20using%20internal%20calibration%2C%20exactly%20as%20is%20required%20for%20actual%20samples.%20Since%20most%20fitted%20sensitivities%20were%20biased%20low%2C%20systematic%20error%20in%20inferred%20ambient%20%5BM-f%5D%20and%20in%20estimated%20weak%20ligand%20%28L-2%29%20concentrations%20resulted.%20The%20main%20distinction%20between%20the%20mathematical%20approaches%20taken%20by%20participants%20lies%20in%20the%20functional%20form%20of%20the%20speciation%20model%20equations%2C%20with%20their%20implicit%20definition%20of%20independent%20and%20dependent%20or%20manipulated%20variables.%20In%20%27direct%20modeling%27%2C%20the%20dependent%20variable%20is%20the%20measured%20%5BM-f%5D%20%28or%20I-p%29%20and%20the%20total%20metal%20concentration%20%28%5BM%5D%28T%29%29%20is%20considered%20independent%20In%20other%2C%20much%20more%20widely%20used%20methods%20of%20analyzing%20titration%20data%20-%20classical%20linearization%2C%20best%20known%20as%20van%20den%20Berg%5C%2FRuzic%20and%20isotherm%20fitting%20by%20nonlinear%20regression%2C%20best%20known%20as%20the%20langmuir%20or%20Gerringa%20methods%20-%20%5BM-f%5D%20is%20defined%20as%20independent%20and%20the%20dependent%20variable%20calculated%20from%20both%20%5BM%5D%28T%29%20and%20%5BM-f%5D.%20Close%20inspection%20of%20the%20biases%20and%20variability%20in%20the%20estimates%20of%20ligand%20parameters%20and%20in%20predictions%20of%20ambient%20%5BM-f%5D%20revealed%20that%20the%20best%20results%20were%20obtained%20by%20the%20direct%20approach.%20Linear%20regression%20of%20transformed%20data%20yielded%20the%20largest%20bias%20and%20greatest%20variability%2C%20while%20non-linear%20isotherm%20fitting%20generated%20results%20with%20mean%20bias%20comparable%20to%20direct%20modeling%2C%20but%20also%20with%20greater%20variability.%20Participants%20that%20performed%20a%20unified%20analysis%20of%20ACSV%20titration%20curves%20at%20multiple%20detection%20windows%20for%20a%20sample%20improved%20their%20results%20regardless%20of%20the%20basic%20mathematical%20approach%20taken.%20Overall%2C%20the%20three%20most%20accurate%20sets%20of%20results%20were%20obtained%20using%20direct%20modeling%20of%20the%20unified%20multiwindow%20dataset%2C%20while%20the%20single%20most%20accurate%20set%20of%20results%20also%20included%20simultaneous%20calibration.%20We%20therefore%20recommend%20that%20where%20sample%20volume%20and%20time%20permit%2C%20titration%20experiments%20for%20all%20natural%20water%20samples%20be%20designed%20to%20include%20two%20or%20more%20detection%20windows%2C%20especially%20for%20coastal%20and%20estuarine%20waters.%20It%20is%20vital%20that%20more%20practical%20experimental%20designs%20for%20multi-window%20titrations%20be%20developed.%20Finally%2C%20while%20all%20mathematical%20approaches%20proved%20to%20be%20adequate%20for%20some%20datasets%2C%20matrix-based%20equilibrium%20models%20proved%20to%20be%20most%20naturally%20suited%20for%20the%20most%20challenging%20cases%20encountered%20in%20this%20work%2C%20i.e.%2C%20experiments%20where%20the%20added%20ligand%20in%20ACSV%20became%20titrated.%20The%20ProMCC%20program%20%28Omanovic%20et%20al.%2C%20this%20issue%29%20as%20well%20as%20the%20Excel%20Add-in%20based%20KINETEQL%20Multiwindow%20Solver%20spreadsheet%20%28Hudson%2C%202014%29%20have%20this%20capability%20and%20have%20been%20made%20available%20for%20public%20use%20as%20a%20result%20of%20this%20intercomparison%20exercise.%20%28C%29%202015%20The%20Authors.%20Published%20by%20Elsevier%20B.V.%22%2C%22date%22%3A%222015%5C%2F07%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.marchem.2015.03.006%22%2C%22ISSN%22%3A%220304-4203%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A15Z%22%7D%7D%2C%7B%22key%22%3A%22VJB94N6K%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Fitzsimmons%20et%20al.%22%2C%22parsedDate%22%3A%222015-07%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EFitzsimmons%2C%20J.%20N.%2C%20Bundy%2C%20R.%20M.%2C%20Al-Subiai%2C%20S.%20N.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20%26amp%3B%20Boyle%2C%20E.%20A.%20%282015%29.%20The%20composition%20of%20dissolved%20iron%20in%20the%20dusty%20surface%20ocean%3A%20An%20exploration%20using%20size-fractionated%20iron-binding%20ligands.%20%3Ci%3EMarine%20Chemistry%3C%5C%2Fi%3E%2C%20%3Ci%3E173%3C%5C%2Fi%3E%2C%20125%26%23x2013%3B135.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2014.09.002%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2014.09.002%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20composition%20of%20dissolved%20iron%20in%20the%20dusty%20surface%20ocean%3A%20An%20exploration%20using%20size-fractionated%20iron-binding%20ligands%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20N.%22%2C%22lastName%22%3A%22Fitzsimmons%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%20N.%22%2C%22lastName%22%3A%22Al-Subiai%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20A.%22%2C%22lastName%22%3A%22Boyle%22%7D%5D%2C%22abstractNote%22%3A%22The%20size%20partitioning%20of%20dissolved%20iron%20and%20organic%20iron-binding%20ligands%20into%20soluble%20and%20colloidal%20phases%20was%20investigated%20in%20the%20upper%20150%20m%20of%20two%20stations%20along%20the%20GA03%20U.S.%20GEOTRACES%20North%20Atlantic%20transect.%20The%20size%20fractionation%20was%20completed%20using%20cross-flow%20filtration%20methods%2C%20followed%20by%20analysis%20by%20isotope%20dilution%20inductively-coupled%20plasma%20mass%20spectrometry%20%28ID-ICP-MS%29%20for%20iron%20and%20competitive%20ligand%20exchange-adsorptive%20cathodic%20stripping%20voltammetry%20%28CLE-ACSV%29%20for%20iron-binding%20ligands.%20On%20average%2C%2080%25%20of%20the%200.1-0.65%20nM%20dissolved%20iron%20%28%3C0.2%20mu%20m%29%20was%20partitioned%20into%20the%20colloidal%20iron%20%28cFe%29%20size%20fraction%20%2810%20kDa%20%3C%20cFe%20%3C0.2%20gm%29%2C%20as%20expected%20for%20areas%20of%20the%20ocean%20underlying%20a%20dust%20plume.%20The%201.3-2.0%20nM%20strong%20organic%20iron-binding%20ligands%2C%20however%2C%20overwhelmingly%20%2875-77%25%29%20fell%20into%20the%20soluble%20size%20fraction%20%28%3C10%20kDa%29.%20As%20a%20result%2C%20modeling%20the%20dissolved%20iron%20size%20fractionation%20at%20equilibrium%20using%20the%20observed%20ligand%20partitioning%20did%20not%20accurately%20predict%20the%20iron%20partitioning%20into%20colloidal%20and%20soluble%20pools.%20This%20suggests%20that%20either%20a%20portion%20of%20colloidal%20ligands%20is%20missed%20by%20current%20electrochemical%20methods%20because%20they%20react%20with%20iron%20more%20slowly%20than%20the%20equilibration%20time%20of%20our%20CLE-ACSV%20method%2C%20or%20part%20of%20the%20observed%20colloidal%20iron%20is%20actually%20inorganic%20in%20composition%20and%20thus%20cannot%20be%20predicted%20by%20our%20model%20of%20unbound%20iron-binding%20ligands.%20This%20potentially%20contradicts%20the%20prevailing%20view%20that%20greater%20than%20%3E99%25%20of%20dissolved%20iron%20in%20the%20ocean%20is%20organically%20complexed.%20Disentangling%20the%20chemical%20form%20of%20iron%20in%20the%20upper%20ocean%20has%20important%20implications%20for%20surface%20ocean%20biogeochemistry%20and%20may%20affect%20iron%20uptake%20by%20phytoplankton.%20%28C%29%202014%20Elsevier%20B.V.%20All%20rights%20reserved.%22%2C%22date%22%3A%222015%5C%2F07%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.marchem.2014.09.002%22%2C%22ISSN%22%3A%220304-4203%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A14Z%22%7D%7D%2C%7B%22key%22%3A%22VNSMP9HP%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Semeniuk%20et%20al.%22%2C%22parsedDate%22%3A%222015-07%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESemeniuk%2C%20D.%20M.%2C%20Bundy%2C%20R.%20M.%2C%20Payne%2C%20C.%20D.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20%26amp%3B%20Maldonado%2C%20M.%20T.%20%282015%29.%20Acquisition%20of%20organically%20complexed%20copper%20by%20marine%20phytoplankton%20and%20bacteria%20in%20the%20northeast%20subarctic%20Pacific%20Ocean.%20%3Ci%3EMarine%20Chemistry%3C%5C%2Fi%3E%2C%20%3Ci%3E173%3C%5C%2Fi%3E%2C%20222%26%23x2013%3B233.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2015.01.005%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2015.01.005%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Acquisition%20of%20organically%20complexed%20copper%20by%20marine%20phytoplankton%20and%20bacteria%20in%20the%20northeast%20subarctic%20Pacific%20Ocean%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%20M.%22%2C%22lastName%22%3A%22Semeniuk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20D.%22%2C%22lastName%22%3A%22Payne%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20T.%22%2C%22lastName%22%3A%22Maldonado%22%7D%5D%2C%22abstractNote%22%3A%22Copper%20%28Cu%29%20is%20an%20essential%20micronutrient%20for%20marine%20phytoplankton%2C%20but%20can%20cause%20toxicity%20at%20elevated%20intracellular%20concentrations.%20The%20majority%20of%20Cu%20%28%3E99.9%25%29%20in%20oceanic%20surface%20waters%20is%20bound%20to%20strong%20organic%20ligands%2C%20presumably%20produced%20by%20prokaryotes%20to%20detoxify%20Cu.%20Although%20laboratory%20studies%20have%20demonstrated%20that%20organically%20complexed%20Cu%20may%20be%20bioavailable%20to%20marine%20eukaryotic%20phytoplankton%2C%20the%20bioavailability%20of%20Cu%20organic%20complexes%20to%20indigenous%20marine%20phytoplankton%20has%20not%20been%20examined%20in%20detail.%20Using%20the%20carrier%20free%20radioisotope%20Cu-67%20at%20an%20iron%20limited%20station%20in%20the%20northeast%20subarctic%20Pacific%20Ocean%2C%20we%20performed%20size%20fractionated%20short-term%20Cu%20uptake%20assays%20with%20three%20Cu%28II%29-chelates%2C%20and%20Cu-67%20bound%20to%20the%20strong%20in%20situ%20ligands%2C%20with%20or%20without%20additions%20of%20weak%20Cu%28I%29%20ligands.%20Estimates%20of%20the%20maximum%20supply%20of%20inorganic%20Cu%20%28Cu%27%29%20to%20the%20cell%20surface%20of%20eukaryotic%20phytoplankton%20were%20unable%20to%20account%20for%20the%20observed%20Cu%20uptake%20rates%20from%20the%20in%20situ%20ligands%20and%20two%20of%20the%20three%20added%20Cu%28II%29-chelates.%20Addition%20of%2010%20nM%20weak%20organic%20Cu%28I%29%20ligands%20enhanced%20uptake%20of%20Cu%20bound%20to%20the%20in%20situ%20ligands.%20Thus%2C%20Cu%20within%20the%20in%20situ%20and%20strong%20artificial%20Cu%28II%29%20organic%20ligands%20was%20accessible%20to%20the%20phytoplankton%20community%20via%20various%20possible%20Cu%20uptake%20strategies%2C%20including%3B%20cell%20surface%20enzymatically%20mediated%20reduction%20of%20Cu%28II%29%20to%20Cu%28I%29%2C%20the%20substrate%20of%20the%20high-affinity%20Cu%20transport%20system%20in%20eukaryotes%3B%20or%20ligand%20exchange%20between%20weak%20Cu-binding%20ligands%20and%20the%20cellular%20Cu%20transporters.%20During%20a%2014-hour%20uptake%20assay%2C%20particulate%20Cu%20concentrations%20reached%20a%20plateau%20in%20most%20treatments.%20Losses%20were%20observed%20in%20some%20treatments%2C%20especially%20in%20the%20small%20size%20fractions%20%28%3C5%20mu%20m%29%2C%20corresponding%20with%20faster%20initial%20Cu%20uptake%20rates.%20This%20may%20indicate%20that%20Cu%20cycling%20is%20rapid%20between%20particulate%20and%20dissolved%20phases%20due%20to%20cellular%20efflux%20or%20remineralization%20by%20micrograzers.%20The%20acquisition%20of%20Cu%20from%20the%20strong%20in%20situ%20ligands%20puts%20into%20question%20the%20historic%20role%20attributed%20to%20Cu%20binding%20ligands%20in%20decreasing%20Cu%20bioavailability.%20%28C%29%202015%20Elsevier%20B.V.%20All%20rights%20reserved.%22%2C%22date%22%3A%222015%5C%2F07%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.marchem.2015.01.005%22%2C%22ISSN%22%3A%220304-4203%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A14Z%22%7D%7D%2C%7B%22key%22%3A%22HBI6ILAJ%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Dupont%20et%20al.%22%2C%22parsedDate%22%3A%222014-10%22%2C%22numChildren%22%3A6%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EDupont%2C%20C.%20L.%2C%20McCrow%2C%20J.%20P.%2C%20Valas%2C%20R.%2C%20Moustafa%2C%20A.%2C%20Walworth%2C%20N.%2C%20Goodenough%2C%20U.%2C%20Roth%2C%20R.%2C%20Hogle%2C%20S.%20L.%2C%20Bai%2C%20J.%2C%20Johnson%2C%20Z.%20I.%2C%20Mann%2C%20E.%2C%20Palenik%2C%20B.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20Craig%20Venter%2C%20J.%2C%20%26amp%3B%20Allen%2C%20A.%20E.%20%282014%29.%20Genomes%20and%20gene%20expression%20across%20light%20and%20productivity%20gradients%20in%20eastern%20subtropical%20Pacific%20microbial%20communities.%20%3Ci%3EIsme%20Journal%3C%5C%2Fi%3E%2C%20%3Ci%3E9%3C%5C%2Fi%3E%285%29%2C%201076%26%23x2013%3B1092.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fismej.2014.198%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fismej.2014.198%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Genomes%20and%20gene%20expression%20across%20light%20and%20productivity%20gradients%20in%20eastern%20subtropical%20Pacific%20microbial%20communities%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chris%20L.%22%2C%22lastName%22%3A%22Dupont%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22John%20P.%22%2C%22lastName%22%3A%22McCrow%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ruben%22%2C%22lastName%22%3A%22Valas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ahmed%22%2C%22lastName%22%3A%22Moustafa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nathan%22%2C%22lastName%22%3A%22Walworth%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ursula%22%2C%22lastName%22%3A%22Goodenough%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robyn%22%2C%22lastName%22%3A%22Roth%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shane%20L.%22%2C%22lastName%22%3A%22Hogle%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jing%22%2C%22lastName%22%3A%22Bai%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zackary%20I.%22%2C%22lastName%22%3A%22Johnson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elizabeth%22%2C%22lastName%22%3A%22Mann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brian%22%2C%22lastName%22%3A%22Palenik%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katherine%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Craig%20Venter%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%20E.%22%2C%22lastName%22%3A%22Allen%22%7D%5D%2C%22abstractNote%22%3A%22Transitions%20in%20community%20genomic%20features%20and%20biogeochemical%20processes%20were%20examined%20in%20surface%20and%20subsurface%20chlorophyll%20maximum%20%28SCM%29%20microbial%20communities%20across%20a%20trophic%20gradient%20from%20mesotrophic%20waters%20near%20San%20Diego%2C%20California%20to%20the%20oligotrophic%20Pacific.%20Transect%20end%20points%20contrasted%20in%20thermocline%20depth%2C%20rates%20of%20nitrogen%20and%20CO2%20uptake%2C%20new%20production%20and%20SCM%20light%20intensity.%20Relative%20to%20surface%20waters%2C%20bacterial%20SCM%20communities%20displayed%20greater%20genetic%20diversity%20and%20enrichment%20in%20putative%20sulfur%20oxidizers%2C%20multiple%20actinomycetes%2C%20low-light-adapted%20Prochlorococcus%20and%20cell-associated%20viruses.%20Metagenomic%20coverage%20was%20not%20correlated%20with%20transcriptional%20activity%20for%20several%20key%20taxa%20within%20Bacteria.%20Low-light-adapted%20Prochlorococcus%2C%20Synechococcus%2C%20and%20low%20abundance%20gamma-proteobacteria%20enriched%20in%20the%3E3.0-%5Bmu%5Dm%20size%20fraction%20contributed%20disproportionally%20to%20global%20transcription.%20The%20abundance%20of%20these%20groups%20also%20correlated%20with%20community%20functions%2C%20such%20as%20primary%20production%20or%20nitrate%20uptake.%20In%20contrast%2C%20many%20of%20the%20most%20abundant%20bacterioplankton%2C%20including%20SAR11%2C%20SAR86%2C%20SAR112%20and%20high-light-adapted%20Prochlorococcus%2C%20exhibited%20low%20levels%20of%20transcriptional%20activity%20and%20were%20uncorrelated%20with%20rate%20processes.%20Eukaryotes%20such%20as%20Haptophytes%20and%20non-photosynthetic%20Aveolates%20were%20prevalent%20in%20surface%20samples%20while%20Mamielles%20and%20Pelagophytes%20dominated%20the%20SCM.%20Metatranscriptomes%20generated%20with%20ribosomal%20RNA-depleted%20mRNA%20%28total%20mRNA%29%20coupled%20to%20in%20vitro%20polyadenylation%20compared%20with%20polyA-enriched%20mRNA%20revealed%20a%20trade-off%20in%20detection%20eukaryotic%20organelle%20and%20eukaryotic%20nuclear%20origin%20transcripts%2C%20respectively.%20Gene%20expression%20profiles%20of%20SCM%20eukaryote%20populations%2C%20highly%20similar%20in%20sequence%20identity%20to%20the%20model%20pelagophyte%20Pelagomonas%20sp.%20CCMP1756%2C%20suggest%20that%20pelagophytes%20are%20responsible%20for%20a%20majority%20of%20nitrate%20assimilation%20within%20the%20SCM.%22%2C%22date%22%3A%222014%5C%2F10%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fismej.2014.198%22%2C%22ISSN%22%3A%221751-7362%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%2C%2286H9SNJB%22%2C%22BSCQ9CW7%22%5D%2C%22dateModified%22%3A%222024-04-15T17%3A26%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22J7NNB4BI%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Roe%20and%20Barbeau%22%2C%22parsedDate%22%3A%222014-10%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ERoe%2C%20K.%20L.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282014%29.%20Uptake%20mechanisms%20for%20inorganic%20iron%20and%20ferric%20citrate%20in%20Trichodesmium%20erythraeum%20IMS101.%20%3Ci%3EMetallomics%3C%5C%2Fi%3E%2C%20%3Ci%3E6%3C%5C%2Fi%3E%2811%29%2C%202042%26%23x2013%3B2051.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1039%5C%2Fc4mt00026a%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1039%5C%2Fc4mt00026a%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Uptake%20mechanisms%20for%20inorganic%20iron%20and%20ferric%20citrate%20in%20Trichodesmium%20erythraeum%20IMS101%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20L.%22%2C%22lastName%22%3A%22Roe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22Growth%20of%20the%20prevalent%20marine%20organism%20Trichodesmium%20can%20be%20limited%20by%20iron%20in%20natural%20and%20laboratory%20settings.%20This%20study%20investigated%20the%20iron%20uptake%20mechanisms%20that%20the%20model%20organism%20T.%20erythraeum%20IMS101%20uses%20to%20acquire%20iron%20from%20inorganic%20iron%20and%20iron%20associated%20with%20the%20weak%20ligand%20complex%2C%20ferric%20citrate.%20IMS101%20was%20observed%20to%20employ%20two%20different%20iron%20uptake%20mechanisms%3A%20superoxide-mediated%20reduction%20of%20inorganic%20iron%20in%20the%20surrounding%20milieu%20and%20a%20superoxide-independent%20uptake%20system%20for%20ferric%20citrate%20complexes.%20While%20the%20detailed%20pathway%20of%20ferric%20citrate%20utilization%20remains%20to%20be%20elucidated%2C%20transport%20of%20iron%20from%20this%20complex%20appears%20to%20involve%20reduction%20and%5C%2For%20exchange%20of%20the%20iron%20out%20of%20the%20complex%20prior%20to%20uptake%2C%20either%20at%20the%20outer%20membrane%20of%20the%20cell%20or%20within%20the%20periplasmic%20space.%20Various%20iron%20uptake%20strategies%20may%20allow%20Trichodesmium%20to%20effectively%20scavenge%20iron%20in%20oligotrophic%20ocean%20environments.%22%2C%22date%22%3A%222014%5C%2F10%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1039%5C%2Fc4mt00026a%22%2C%22ISSN%22%3A%221756-5901%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A17Z%22%7D%7D%2C%7B%22key%22%3A%22UMRF2TDE%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Bundy%20et%20al.%22%2C%22parsedDate%22%3A%222014-05%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBundy%2C%20R.%20M.%2C%20Biller%2C%20D.%20V.%2C%20Buck%2C%20K.%20N.%2C%20Bruland%2C%20K.%20W.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282014%29.%20Distinct%20pools%20of%20dissolved%20iron-binding%20ligands%20in%20the%20surface%20and%20benthic%20boundary%20layer%20of%20the%20California%20Current.%20%3Ci%3ELimnology%20and%20Oceanography%3C%5C%2Fi%3E%2C%20%3Ci%3E59%3C%5C%2Fi%3E%283%29%2C%20769%26%23x2013%3B787.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.4319%5C%2Flo.2014.59.3.0769%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.4319%5C%2Flo.2014.59.3.0769%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Distinct%20pools%20of%20dissolved%20iron-binding%20ligands%20in%20the%20surface%20and%20benthic%20boundary%20layer%20of%20the%20California%20Current%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%20V.%22%2C%22lastName%22%3A%22Biller%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20N.%22%2C%22lastName%22%3A%22Buck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20W.%22%2C%22lastName%22%3A%22Bruland%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22Organic%20dissolved%20iron%20%28dFe%29-binding%20ligands%20were%20measured%20by%20competitive%20ligand%20exchange-adsorptive%20cathodic%20stripping%20voltammetry%20%28CLE-ACSV%29%20at%20multiple%20analytical%20windows%20%28side%20reaction%20coefficient%20of%20salicylaldoxime%2C%20alpha%28Fe%28SA%292%29%20%3D%2030%2C%2060%2C%20and%20100%29%20in%20surface%20and%20benthic%20boundary%20layer%20%28BBL%29%20samples%20along%20the%20central%20California%20coast%20during%20spring%20and%20summer.%20The%20weakest%20ligands%20were%20detected%20in%20the%20BBL%20at%20the%20lowest%20analytical%20window%20with%20average%20log%20K-FeL%2CFe%27%28cond%29%20%3D%2010.2%20%2B%5C%2F-%200.4%20in%20the%20summer%20and%2010.8%20%2B%5C%2F-%200.2%20in%20the%20spring.%20Between%203%25%20and%2018%25%20of%20the%20dFe%20complexation%20in%20the%20BBL%20was%20accounted%20for%20by%20HS%2C%20which%20were%20measured%20separately%20in%20samples%20by%20ACSV%20and%20may%20indicate%20a%20source%20of%20dFe-binding%20ligands%20from%20San%20Francisco%20Bay.%20The%20strongest%20ligands%20were%20found%20in%20nearshore%20spring%20surface%20waters%20at%20the%20highest%20analytical%20window%20with%20average%20log%20K-FeL%2CFe%27%28cond%29%20%3D%2011.9%20%2B%5C%2F-%200.3%2C%20and%20the%20concentrations%20of%20these%20ligands%20declined%20rapidly%20offshore.%20The%20ligand%20pools%20in%20the%20surface%20and%20BBL%20waters%20were%20distinct%20from%20each%20other%20based%20on%20principal%20components%20analysis%2C%20with%20variances%20in%20the%20BBL%20ligand%20pool%20explained%20by%20sample%20location%2C%20and%20variance%20in%20surface%20waters%20explained%20by%20water%20mass.%20The%20use%20of%20multiple%20analytical%20window%20analysis%20elucidated%20several%20distinct%20iron-binding%20ligand%20pools%2C%20each%20with%20unique%20distributions%20in%20the%20central%20California%20Current%20system.%22%2C%22date%22%3A%222014%5C%2F05%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.4319%5C%2Flo.2014.59.3.0769%22%2C%22ISSN%22%3A%220024-3590%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A14Z%22%7D%7D%2C%7B%22key%22%3A%22TPKCBUTA%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hogle%20et%20al.%22%2C%22parsedDate%22%3A%222014-04%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHogle%2C%20S.%20L.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20%26amp%3B%20Gledhill%2C%20M.%20%282014%29.%20Heme%20in%20the%20marine%20environment%3A%20from%20cells%20to%20the%20iron%20cycle.%20%3Ci%3EMetallomics%3C%5C%2Fi%3E%2C%20%3Ci%3E6%3C%5C%2Fi%3E%286%29%2C%201107%26%23x2013%3B1120.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1039%5C%2Fc4mt00031e%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1039%5C%2Fc4mt00031e%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Heme%20in%20the%20marine%20environment%3A%20from%20cells%20to%20the%20iron%20cycle%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%20L.%22%2C%22lastName%22%3A%22Hogle%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Gledhill%22%7D%5D%2C%22abstractNote%22%3A%22Hemes%20are%20iron%20containing%20heterocyclic%20molecules%20important%20in%20many%20cellular%20processes.%20In%20the%20marine%20environment%2C%20hemes%20participate%20as%20enzymatic%20cofactors%20in%20biogeochemically%20significant%20processes%20like%20photosynthesis%2C%20respiration%2C%20and%20nitrate%20assimilation.%20Further%2C%20hemoproteins%2C%20hemes%2C%20and%20their%20analogs%20appear%20to%20be%20iron%20sources%20for%20some%20marine%20bacterioplankton%20under%20certain%20conditions.%20Current%20oceanographic%20analytical%20methodologies%20allow%20for%20the%20extraction%20and%20measurement%20of%20heme%20b%20from%20marine%20material%2C%20and%20a%20handful%20of%20studies%20have%20begun%20to%20examine%20the%20distribution%20of%20heme%20b%20in%20ocean%20basins.%20The%20study%20of%20heme%20in%20the%20marine%20environment%20is%20still%20in%20its%20infancy%2C%20but%20some%20trends%20can%20be%20gleaned%20from%20the%20work%20that%20has%20been%20published%20so%20far.%20In%20this%20review%2C%20we%20summarize%20what%20is%20known%20or%20might%20be%20inferred%20about%20the%20roles%20of%20heme%20in%20marine%20microbes%20as%20well%20as%20the%20few%20studies%20on%20heme%20in%20the%20marine%20environment%20that%20have%20been%20conducted%20to%20date.%20We%20conclude%20by%20presenting%20some%20future%20questions%20and%20challenges%20for%20the%20field.%22%2C%22date%22%3A%222014%5C%2F04%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1039%5C%2Fc4mt00031e%22%2C%22ISSN%22%3A%221756-5901%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A15Z%22%7D%7D%2C%7B%22key%22%3A%223FTL3B2N%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Earley%20et%20al.%22%2C%22parsedDate%22%3A%222014-01%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EEarley%2C%20P.%20J.%2C%20Swope%2C%20B.%20L.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%2C%20Bundy%2C%20R.%2C%20McDonald%2C%20J.%20A.%2C%20%26amp%3B%20Rivera-Duarte%2C%20I.%20%282014%29.%20Life%20cycle%20contributions%20of%20copper%20from%20vessel%20painting%20and%20maintenance%20activities.%20%3Ci%3EBiofouling%3C%5C%2Fi%3E%2C%20%3Ci%3E30%3C%5C%2Fi%3E%281%29%2C%2051%26%23x2013%3B68.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1080%5C%2F08927014.2013.841891%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1080%5C%2F08927014.2013.841891%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Life%20cycle%20contributions%20of%20copper%20from%20vessel%20painting%20and%20maintenance%20activities%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%20J.%22%2C%22lastName%22%3A%22Earley%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20L.%22%2C%22lastName%22%3A%22Swope%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20A.%22%2C%22lastName%22%3A%22McDonald%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22I.%22%2C%22lastName%22%3A%22Rivera-Duarte%22%7D%5D%2C%22abstractNote%22%3A%22Copper-based%20epoxy%20and%20ablative%20antifouling%20painted%20panels%20were%20exposed%20in%20natural%20seawater%20to%20evaluate%20environmental%20loading%20parameters.%20In%20situ%20loading%20factors%20including%20initial%20exposure%2C%20passive%20leaching%2C%20and%20surface%20refreshment%20were%20measured%20utilizing%20two%20protocols%20developed%20by%20the%20US%20Navy%3A%20the%20dome%20method%20and%20the%20in-water%20hull%20cleaning%20sampling%20method.%20Cleaning%20techniques%20investigated%20included%20a%20soft-pile%20carpet%20and%20a%20medium%20duty%203M%28%28TM%29%29%20pad%20for%20fouling%20removal.%20Results%20show%20that%20the%20passive%20leach%20rates%20of%20copper%20peaked%20three%20days%20after%20both%20initial%20deployment%20and%20cleaning%20events%20%28CEs%29%2C%20followed%20by%20a%20rapid%20decrease%20over%20about%2015days%20and%20a%20slow%20approach%20to%20asymptotic%20levels%20on%20approximately%20day%2030.%20Additionally%2C%20copper%20was%20more%20bioavailable%20during%20a%20CE%20in%20comparison%20to%20the%20passive%20leaching%20that%20immediately%20followed.%20A%20paint%20life%20cycle%20model%20quantifying%20annual%20copper%20loading%20estimates%20for%20each%20paint%20and%20cleaning%20method%20based%20on%20a%20three-year%20cycle%20of%20painting%2C%20episodic%20cleaning%2C%20and%20passive%20leaching%20is%20presented.%22%2C%22date%22%3A%222014%5C%2F01%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1080%5C%2F08927014.2013.841891%22%2C%22ISSN%22%3A%220892-7014%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A15Z%22%7D%7D%2C%7B%22key%22%3A%22XN4VR2G9%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Roe%20et%20al.%22%2C%22parsedDate%22%3A%222013-09%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ERoe%2C%20K.%20L.%2C%20Hogle%2C%20S.%20L.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282013%29.%20Utilization%20of%20heme%20as%20an%20iron%20source%20by%20marine%20alphaproteobacteria%20in%20the%20roseobacter%20clade.%20%3Ci%3EApplied%20and%20Environmental%20Microbiology%3C%5C%2Fi%3E%2C%20%3Ci%3E79%3C%5C%2Fi%3E%2818%29%2C%205753%26%23x2013%3B5762.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Faem.01562-13%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Faem.01562-13%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Utilization%20of%20heme%20as%20an%20iron%20source%20by%20marine%20alphaproteobacteria%20in%20the%20roseobacter%20clade%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20L.%22%2C%22lastName%22%3A%22Roe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%20L.%22%2C%22lastName%22%3A%22Hogle%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22The%20bioavailability%20and%20utilization%20of%20porphyrin-bound%20iron%2C%20specifically%20heme%2C%20by%20marine%20microorganisms%20have%20rarely%20been%20examined.%20This%20study%20used%20Ruegeria%20sp.%20strain%20TrichCH4B%20as%20a%20model%20organism%20to%20study%20heme%20acquisition%20by%20a%20member%20of%20the%20Roseobacter%20clade.%20Analogs%20of%20known%20heme%20transporter%20proteins%20were%20found%20within%20the%20Ruegeria%20sp.%20TrichCH4B%20genome.%20The%20identified%20heme%20uptake%20and%20utilization%20system%20appears%20to%20be%20functional%2C%20as%20the%20heme%20genes%20were%20upregulated%20under%20iron%20stress%2C%20the%20bacterium%20could%20grow%20on%20ferric-porphyrin%20complexes%20as%20the%20sole%20iron%20source%2C%20and%20internalization%20of%2855%29%20Fe%20from%20ferric%20protoporphyrin%20IX%20was%20observed.%20The%20potential%20ability%20to%20utilize%20heme%20in%20the%20Roseobacter%20clade%20appears%20to%20be%20common%2C%20as%20half%20of%20the%20isolates%20in%20the%20RoseoBase%20database%20were%20found%20to%20have%20a%20complete%20heme%20uptake%20system.%20A%20degenerate%20primer%20set%20was%20designed%20and%20successfully%20used%20to%20identify%20the%20putative%20heme%20oxygenase%20gene%20%28hmus%29%20in%20the%20roseobacter%20heme%20uptake%20system%20from%20diverse%20nonenriched%20marine%20environments.%20This%20study%20found%20that%20members%20of%20the%20Roseobacter%20clade%20are%20capable%20of%20utilizing%20heme%20as%20an%20iron%20source%20and%20that%20this%20capability%20may%20be%20present%20in%20all%20types%20of%20marine%20environments.%20The%20results%20of%20this%20study%20add%20a%20new%20perspective%20to%20the%20current%20picture%20of%20iron%20cycling%20in%20marine%20systems%2C%20whereby%20relatively%20refractory%20intracellular%20pools%20of%20heme-bound%20iron%20may%20be%20taken%20up%20quickly%20and%20directly%20reincorporated%20into%20living%20bacteria%20without%20previous%20degradation%20or%20the%20necessity%20of%20a%20siderophore%20intermediate.%22%2C%22date%22%3A%222013%5C%2F09%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1128%5C%2Faem.01562-13%22%2C%22ISSN%22%3A%220099-2240%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A17Z%22%7D%7D%2C%7B%22key%22%3A%22EBEYN93V%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Ohman%20et%20al.%22%2C%22parsedDate%22%3A%222013-09%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EOhman%2C%20M.%20D.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%2C%20Franks%2C%20P.%20J.%20S.%2C%20Goericke%2C%20R.%2C%20Landry%2C%20M.%20R.%2C%20%26amp%3B%20Miller%2C%20A.%20J.%20%282013%29.%20Ecological%20transitions%20in%20a%20coastal%20upwelling%20ecosystem.%20%3Ci%3EOceanography%3C%5C%2Fi%3E%2C%20%3Ci%3E26%3C%5C%2Fi%3E%283%29%2C%20210%26%23x2013%3B219.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Ecological%20transitions%20in%20a%20coastal%20upwelling%20ecosystem%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20D.%22%2C%22lastName%22%3A%22Ohman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%20J.%20S.%22%2C%22lastName%22%3A%22Franks%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Goericke%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20R.%22%2C%22lastName%22%3A%22Landry%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20J.%22%2C%22lastName%22%3A%22Miller%22%7D%5D%2C%22abstractNote%22%3A%22The%20southern%20California%20Current%20Ecosystem%20%28CCE%29%20is%20a%20dynamic%20eastern%20boundary%20current%20ecosystem%20that%20is%20forced%20by%20ocean-atmosphere%20variability%20on%20interannual%2C%20multidecadal%2C%20and%20long-term%20secular%20time%20scales.%20Recent%20evidence%20suggests%20that%20apparent%20abrupt%20transitions%20in%20ecosystem%20conditions%20reflect%20linear%20tracking%20of%20the%20physical%20environment%20rather%20than%20oscillations%20between%20alternative%20preferred%20states.%20A%20space-for-time%20exchange%20is%20one%20approach%20that%20permits%20use%20of%20natural%20spatial%20variability%20in%20the%20CCE%20to%20develop%20a%20mechanistic%20understanding%20needed%20to%20project%20future%20temporal%20changes.%20The%20role%20of%20%28sub%29mesoscale%20frontal%20systems%20in%20altering%20rates%20of%20nutrient%20transport%2C%20primary%20and%20secondary%20production%2C%20export%20fluxes%2C%20and%20the%20rates%20of%20encounters%20between%20predators%20and%20prey%20is%20an%20issue%20central%20to%20this%20pelagic%20ecosystem%20and%20its%20future%20trajectory%20because%20the%20occurrence%20of%20such%20frontal%20features%20is%20increasing.%22%2C%22date%22%3A%222013%5C%2F09%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%22%22%2C%22ISSN%22%3A%221042-8275%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A14Z%22%7D%7D%2C%7B%22key%22%3A%222PQEAZ5T%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Bundy%20et%20al.%22%2C%22parsedDate%22%3A%222013-06%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBundy%2C%20R.%20M.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20%26amp%3B%20Buck%2C%20K.%20N.%20%282013%29.%20Sources%20of%20strong%20copper-binding%20ligands%20in%20Antarctic%20Peninsula%20surface%20waters.%20%3Ci%3EDeep-Sea%20Research%20Part%20Ii-Topical%20Studies%20in%20Oceanography%3C%5C%2Fi%3E%2C%20%3Ci%3E90%3C%5C%2Fi%3E%2C%20134%26%23x2013%3B146.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.dsr2.2012.07.023%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.dsr2.2012.07.023%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Sources%20of%20strong%20copper-binding%20ligands%20in%20Antarctic%20Peninsula%20surface%20waters%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20N.%22%2C%22lastName%22%3A%22Buck%22%7D%5D%2C%22abstractNote%22%3A%22Copper-binding%20organic%20ligands%20were%20measured%20during%20austral%20winter%20in%20surface%20waters%20around%20the%20Antarctic%20Peninsula%20using%20competitive%20ligand%20exchange-adsorptive%20cathodic%20stripping%20voltammetry%20with%20multiple%20analytical%20windows.%20Samples%20were%20collected%20from%20four%20distinct%20water%20masses%20including%20the%20Antarctic%20Circumpolar%20Current%2C%20Southern%20Antarctic%20Circumpolar%20Current%20Front%2C%20Bransfield%20Strait%2C%20and%20the%20shelf%20region%20of%20the%20Antarctic%20Peninsula.%20Strong%20copper-binding%20organic%20ligands%20were%20detected%20in%20each%20water%20mass.%20The%20strongest%20copper-binding%20ligands%20were%20detected%20at%20the%20highest%20competition%20strength%20in%20the%20Antarctic%20Circumpolar%20Current%2C%20with%20an%20average%20conditional%20stability%20constant%20of%20logK%28CuL%2CCu2%2B%29%28cond%29%20%3D%2016.00%20%2B%5C%2F-%200.82.%20The%20weakest%20ligands%20were%20found%20at%20the%20lowest%20competition%20strength%20in%20the%20shelf%20region%20with%20logK%28CuL%2CCu2%2B%29%28cond%29%20%3D%2012.68%20%2B%5C%2F-%200.48.%20No%20ligands%20with%20stability%20constants%20less%20than%20logK%28CuL%2CCu2%2B%29%28cond%29%20%3D%2013.5%20were%20detected%20in%20the%20Antarctic%20Circumpolar%20Current%20at%20any%20competition%20strength%2C%20suggesting%20a%20shelf%20source%20of%20weaker%20copper-binding%20ligands.%20Free%2C%20hydrated%20copper%20ion%20concentrations%2C%20the%20biologically%20available%20form%20of%20dissolved%20copper%2C%20were%20less%20than%2010%28-14%29%20M%20in%20all%20samples%2C%20approaching%20levels%20that%20may%20be%20limiting%20for%20some%20types%20of%20inducible%20iron%20acquisition.%20%28C%29%202012%20Elsevier%20Ltd.%20All%20rights%20reserved.%22%2C%22date%22%3A%222013%5C%2F06%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.dsr2.2012.07.023%22%2C%22ISSN%22%3A%220967-0645%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A17Z%22%7D%7D%2C%7B%22key%22%3A%223HZRMXKX%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Jiang%20et%20al.%22%2C%22parsedDate%22%3A%222013-06%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EJiang%2C%20M.%20S.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20Selph%2C%20K.%20E.%2C%20Measures%2C%20C.%20I.%2C%20Buck%2C%20K.%20N.%2C%20Azam%2C%20F.%2C%20Mitchell%2C%20B.%20G.%2C%20%26amp%3B%20Zhou%2C%20M.%20%282013%29.%20The%20role%20of%20organic%20ligands%20in%20iron%20cycling%20and%20primary%20productivity%20in%20the%20Antarctic%20Peninsula%3A%20A%20modeling%20study.%20%3Ci%3EDeep-Sea%20Research%20Part%20Ii-Topical%20Studies%20in%20Oceanography%3C%5C%2Fi%3E%2C%20%3Ci%3E90%3C%5C%2Fi%3E%2C%20112%26%23x2013%3B133.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.dsr2.2013.01.029%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.dsr2.2013.01.029%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20role%20of%20organic%20ligands%20in%20iron%20cycling%20and%20primary%20productivity%20in%20the%20Antarctic%20Peninsula%3A%20A%20modeling%20study%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20S.%22%2C%22lastName%22%3A%22Jiang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20E.%22%2C%22lastName%22%3A%22Selph%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20I.%22%2C%22lastName%22%3A%22Measures%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20N.%22%2C%22lastName%22%3A%22Buck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22F.%22%2C%22lastName%22%3A%22Azam%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20G.%22%2C%22lastName%22%3A%22Mitchell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Zhou%22%7D%5D%2C%22abstractNote%22%3A%22Iron%20%28Fe%29%20is%20the%20limiting%20nutrient%20for%20primary%20productivity%20in%20the%20Southern%20Ocean%2C%20with%20much%20of%20the%20dissolved%20iron%20%28dFe%29%20bound%20to%20organic%20ligands%20or%20colloids.%20A%20Fe%20model%20for%20the%20Southern%20Ocean%20%28SOFe%29%20is%20developed%20to%20understand%20the%20role%20of%20bacteria%20and%20organic%20ligands%20in%20controlling%20Fe%20cycling%20and%20productivity.%20The%20model%20resolves%20the%20classical%20food%20web%20and%20microbial%20loop%2C%20including%20three%20types%20of%20nutrients%20%28N%2C%20Si%2C%20Fe%29%20and%20two%20types%20of%20Fe%20ligands.%20Simulations%20of%20the%20zero-dimensional%20%280-D%29%20model%20are%20calibrated%20with%20detailed%20results%20of%20shipboard%20grow-out%20incubation%20experiments%20conducted%20with%20Antarctic%20Peninsula%20phytoplankton%20communities%20during%20winter%202006%20to%20provide%20the%20best%20estimate%20of%20key%20biological%20parameters.%20Then%20a%20one-dimensional%20%281-D%29%20model%20is%20developed%20by%20coupling%20the%20biological%20model%20with%20the%20Regional%20Oceanic%20Modeling%20System%20%28ROMS%29%20for%20a%20site%20on%20the%20Antarctic%20Peninsula%20shelf%2C%20and%20the%20model%20parameters%20are%20further%20calibrated%20with%20data%20collected%20from%20two%20surveys%20%28summer%202004%20and%20winter%202006%29%20in%20the%20area.%20The%20results%20of%20the%20numerical%20simulations%20agree%20reasonably%20well%20with%20observations.%20An%20analysis%20of%20the%201-D%20model%20results%20suggests%20that%20bacteria%20and%20organic%20ligands%20may%20play%20an%20important%20role%20in%20Fe%20cycling%2C%20which%20can%20be%20categorized%20into%20a%20relatively%20fast%20mode%20within%20the%20euphotic%20zone%20dominated%20by%20photo-reactions%20%28summer%20d%20Fe%20residence%20time%20about%20600%20days%29%20and%20complexation%20and%20a%20slow%20mode%20below%20with%20most%20of%20the%20dFe%20biologically%20complexed%20%28summer%20dFe%20residence%20time%20%3E%2010%20years%29.%20The%20dFe%20removal%20from%20the%20euphotic%20zone%20is%20dominated%20by%20colloidal%20formation%20and%20further%20aggregations%20with%20additional%20contribution%20from%20biological%20uptake%2C%20and%20an%20increase%20of%20organic%20ligands%20would%20reduce%20Fe%20export.%20The%20decrease%20of%20Fe%20removal%20rate%20over%20depth%20is%20due%20to%20the%20continuous%20dissolution%20and%20remineralization%20of%20particulate%20Fe.%20A%20number%20of%20sensitivity%20experiments%20are%20carried%20out%20for%20both%20O-D%20and%201-D%20models%20to%20understand%20the%20importance%20of%20photo-reactive%20processes%20in%20primary%20productivity%2C%20bacterial%20activity%2C%20Fe%20speciation%2C%20and%20dFe%20residence%20time%20within%20the%20euphotic%20zone.%20The%20bio-availability%20of%20ligand-bound%20Fe%20%28FeL%29%20is%20critical%20to%20modeled%20high%20primary%20productivity%2C%20which%20is%20consistent%20with%20both%20shipboard%20measurements%20and%20field%20observations.%20In%20addition%2C%20model%20productivity%20is%20sensitive%20to%20photoreaction%20rates%20if%20FeL%20is%20not%20directly%20available%20for%20phytoplankton%20uptake.%20%28C%29%202013%20Elsevier%20Ltd.%20All%20rights%20reserved.%22%2C%22date%22%3A%222013%5C%2F06%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.dsr2.2013.01.029%22%2C%22ISSN%22%3A%220967-0645%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A17Z%22%7D%7D%2C%7B%22key%22%3A%22N88XFC9V%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hopkinson%20et%20al.%22%2C%22parsedDate%22%3A%222013-06%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHopkinson%2C%20B.%20M.%2C%20Seegers%2C%20B.%2C%20Hatta%2C%20M.%2C%20Measures%2C%20C.%20I.%2C%20Mitchell%2C%20B.%20G.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282013%29.%20Planktonic%20C%3AFe%20ratios%20and%20carrying%20capacity%20in%20the%20southern%20Drake%20Passage.%20%3Ci%3EDeep-Sea%20Research%20Part%20Ii-Topical%20Studies%20in%20Oceanography%3C%5C%2Fi%3E%2C%20%3Ci%3E90%3C%5C%2Fi%3E%2C%20102%26%23x2013%3B111.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.dsr2.2012.09.001%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.dsr2.2012.09.001%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Planktonic%20C%3AFe%20ratios%20and%20carrying%20capacity%20in%20the%20southern%20Drake%20Passage%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20M.%22%2C%22lastName%22%3A%22Hopkinson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%22%2C%22lastName%22%3A%22Seegers%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Hatta%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%20I.%22%2C%22lastName%22%3A%22Measures%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20G.%22%2C%22lastName%22%3A%22Mitchell%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22The%20carbon%20to%20iron%20%28C%3AFe%29%20ratio%20of%20planktonic%20biomass%20constrains%20net%20production%20in%20iron-limited%20regions%20of%20the%20ocean%20and%20is%20an%20important%20parameter%20for%20predicting%20biomass%20production%20from%20iron%20inputs.%20On%20a%20cruise%20to%20the%20southern%20Drake%20Passage%20in%20July-August%202006%2C%20we%20used%20two%20approaches%20to%20determine%20the%20C%3AFe%20ratio%20of%20planktonic%20material%3A%20dual-radiotracer%20labeling%20and%20net%20biomass%20production%20in%20iron-limited%20grow-out%20experiments.%20There%20was%20variability%20in%20C%3AFe%20ratios%20among%20experiments%2C%20but%20values%20from%20the%20two%20methods%20overlapped%20with%20average%20values%20of%201.4%20x%2010%285%29%20%28mol%3Amol%29%20for%20the%20radiotracer%20method%20and%201.7%20x%2010%285%29%20for%20the%20net%20biomass%20production%20method.%20This%20is%20notable%20since%20the%20net%20biomass%20production%20method%20is%20a%20new%20approach%20to%20determine%20C%3AFe%20ratios.%20Although%20it%20has%20potential%20issues%20related%20to%20bottle%20effects%20and%20sensitivity%20to%20trace%20contamination%2C%20the%20method%20avoids%20some%20of%20the%20questions%20associated%20with%20iron%20speciation%20and%20bioavailability%20since%20ambient%20iron%20supports%20production.%20Because%20light%20intensity%20is%20known%20to%20affect%20C%3AFe%20ratios%20in%20phytoplankton%20through%20photosynthetic%20iron%20demands%2C%20we%20tested%20the%20effect%20of%20light%20level%20on%20C%3AFe%20in%20Antarctic%20assemblages.%20In%20contrast%20to%20what%20is%20seen%20in%20many%20phytoplankton%20cultures%2C%20C%3AFe%20ratios%20increased%20at%20low-light%2C%20but%20we%20suspect%20that%20this%20is%20due%20to%20initial%20photoinhibition%20of%20the%20low-light%20adapted%20winter%20assemblages%20at%20higher%20light%20levels.%20%28c%29%202012%20Elsevier%20Ltd.%20All%20rights%20reserved.%22%2C%22date%22%3A%222013%5C%2F06%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.dsr2.2012.09.001%22%2C%22ISSN%22%3A%220967-0645%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A17Z%22%7D%7D%2C%7B%22key%22%3A%22H78R3IXW%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Landry%20et%20al.%22%2C%22parsedDate%22%3A%222012-09%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ELandry%2C%20M.%20R.%2C%20Ohman%2C%20M.%20D.%2C%20Goericke%2C%20R.%2C%20Stukel%2C%20M.%20R.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20Bundy%2C%20R.%2C%20%26amp%3B%20Kahru%2C%20M.%20%282012%29.%20Pelagic%20community%20responses%20to%20a%20deep-water%20front%20in%20the%20California%20Current%20Ecosystem%3A%20overview%20of%20the%20A-Front%20Study.%20%3Ci%3EJournal%20of%20Plankton%20Research%3C%5C%2Fi%3E%2C%20%3Ci%3E34%3C%5C%2Fi%3E%289%29%2C%20739%26%23x2013%3B748.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fplankt%5C%2Ffbs025%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fplankt%5C%2Ffbs025%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Pelagic%20community%20responses%20to%20a%20deep-water%20front%20in%20the%20California%20Current%20Ecosystem%3A%20overview%20of%20the%20A-Front%20Study%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20R.%22%2C%22lastName%22%3A%22Landry%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20D.%22%2C%22lastName%22%3A%22Ohman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Goericke%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20R.%22%2C%22lastName%22%3A%22Stukel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Kahru%22%7D%5D%2C%22abstractNote%22%3A%22In%20October%202008%2C%20we%20investigated%20pelagic%20community%20composition%20and%20biomass%2C%20from%20bacteria%20to%20fish%2C%20across%20a%20sharp%20frontal%20gradient%20overlying%20deep%20waters%20south%20of%20Point%20Conception%2C%20California.%20This%20northsouth%20gradient%2C%20which%20we%20called%20A-Front%2C%20was%20formed%20by%20the%20eastward%20flow%20of%20the%20California%20Current%20and%20separated%20cooler%20mesotrophic%20waters%20of%20coastal%20upwelling%20origin%20to%20the%20north%2C%20from%20warm%20oligotrophic%20waters%20of%20likely%20mixed%20subarcticsubtropical%20origin%20to%20the%20south.%20Plankton%20biomass%20and%20phytoplankton%20growth%20rates%20were%20two%20to%20three%20times%20greater%20on%20the%20northern%20side%2C%20and%20primary%20production%20rates%20were%20elevated%205-fold%20to%20the%20north.%20Compared%20with%20either%20of%20the%20adjacent%20waters%2C%20the%20frontal%20interface%20was%20strongly%20enriched%20and%20uniquely%20defined%20by%20a%20subsurface%20bloom%20of%20large%20diatoms%2C%20elevated%20concentrations%20of%20suspension-feeding%20zooplankton%2C%20high%20bioacoustical%20estimates%20of%20pelagic%20fish%20and%20enhanced%20bacterial%20production%20and%20phytoplankton%20biomass%20and%20photosynthetic%20potential.%20Such%20habitats%2C%20though%20small%20in%20areal%20extent%2C%20may%20contribute%20disproportionately%20and%20importantly%20to%20regional%20productivity%2C%20nutrient%20cycling%2C%20carbon%20fluxes%20and%20trophic%20ecology.%20As%20a%20general%20introduction%20to%20the%20A-Front%20study%2C%20we%20provide%20an%20overview%20of%20its%20design%20and%20implementation%2C%20a%20brief%20summary%20of%20major%20findings%20and%20a%20discussion%20of%20potential%20mechanisms%20of%20plankton%20enrichment%20at%20the%20front.%22%2C%22date%22%3A%22Sep%202012%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.1093%5C%2Fplankt%5C%2Ffbs025%22%2C%22ISSN%22%3A%220142-7873%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A16Z%22%7D%7D%2C%7B%22key%22%3A%22DRIX8SY7%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Buck%20et%20al.%22%2C%22parsedDate%22%3A%222012-07%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBuck%2C%20K.%20N.%2C%20Moffett%2C%20J.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%2C%20Bundy%2C%20R.%20M.%2C%20Kondo%2C%20Y.%2C%20%26amp%3B%20Wu%2C%20J.%20F.%20%282012%29.%20The%20organic%20complexation%20of%20iron%20and%20copper%3A%20an%20intercomparison%20of%20competitive%20ligand%20exchange-adsorptive%20cathodic%20stripping%20voltammetry%20%28CLE-ACSV%29%20techniques.%20%3Ci%3ELimnology%20and%20Oceanography-Methods%3C%5C%2Fi%3E%2C%20%3Ci%3E10%3C%5C%2Fi%3E%2C%20496%26%23x2013%3B515.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.4319%5C%2Flom.2012.10.496%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.4319%5C%2Flom.2012.10.496%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20organic%20complexation%20of%20iron%20and%20copper%3A%20an%20intercomparison%20of%20competitive%20ligand%20exchange-adsorptive%20cathodic%20stripping%20voltammetry%20%28CLE-ACSV%29%20techniques%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20N.%22%2C%22lastName%22%3A%22Buck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Moffett%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20M.%22%2C%22lastName%22%3A%22Bundy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Y.%22%2C%22lastName%22%3A%22Kondo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20F.%22%2C%22lastName%22%3A%22Wu%22%7D%5D%2C%22abstractNote%22%3A%22Characterization%20of%20the%20speciation%20of%20iron%20and%20copper%20is%20an%20important%20objective%20of%20the%20GEOTRACES%20Science%20Plan.%20To%20incorporate%20speciation%20measurements%20into%20such%20a%20multinational%20program%2C%20standard%20practices%20must%20be%20adopted%20that%20allow%20data%20from%20multiple%20labs%20to%20be%20synthesized.%20Competitive%20ligand%20exchange-adsorptive%20cathodic%20stripping%20voltammetry%20%28CLE-ACSV%29%20is%20the%20primary%20technique%20employed%20for%20measuring%20metal-binding%20ligands%20and%20determining%20metal%20speciation%20in%20seawater.%20The%20determination%20of%20concentrations%20and%20conditional%20stability%20constants%20of%20metal-binding%20ligands%20is%20particularly%20challenging%2C%20as%20results%20can%20be%20influenced%20both%20by%20experimental%20conditions%20and%20interpretation%20of%20titration%20data.%20Here%2C%20we%20report%20an%20investigation%20between%20four%20laboratories%20to%20study%20the%20speciation%20of%20iron%20and%20copper%20using%20CLE-ACSV.%20Samples%20were%20collected%20on%20the%20GEOTRACES%20II%20intercomparison%20cruise%20in%20the%20North%20Pacific%20Ocean%20in%20May%202009%20at%2030%20degrees%20N%2C%20140%20degrees%20W.%20This%20intercomparison%20was%20carried%20out%20shipboard%20and%20included%20an%20assessment%20of%20the%20viability%20of%20sample%20preservation%20by%20freezing.%20Results%20showed%20that%20consensus%20values%20could%20be%20obtained%20between%20different%20labs%2C%20but%20that%20some%20existing%20practices%20were%20problematic%20and%20require%20further%20attention%20in%20future%20work.%20A%20series%20of%20recommendations%20emerged%20from%20this%20study%20that%20will%20be%20useful%20in%20implementing%20multi-investigator%20programs%20like%20GEOTRACES.%22%2C%22date%22%3A%22Jul%202012%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.4319%5C%2Flom.2012.10.496%22%2C%22ISSN%22%3A%221541-5856%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A16Z%22%7D%7D%2C%7B%22key%22%3A%22QUGHMWZR%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Roe%20et%20al.%22%2C%22parsedDate%22%3A%222012-07%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ERoe%2C%20K.%20L.%2C%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%2C%20Mann%2C%20E.%20L.%2C%20%26amp%3B%20Haygood%2C%20M.%20G.%20%282012%29.%20Acquisition%20of%20iron%20by%20Trichodesmium%20and%20associated%20bacteria%20in%20culture.%20%3Ci%3EEnvironmental%20Microbiology%3C%5C%2Fi%3E%2C%20%3Ci%3E14%3C%5C%2Fi%3E%287%29%2C%201681%26%23x2013%3B1695.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fj.1462-2920.2011.02653.x%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fj.1462-2920.2011.02653.x%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Acquisition%20of%20iron%20by%20Trichodesmium%20and%20associated%20bacteria%20in%20culture%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20L.%22%2C%22lastName%22%3A%22Roe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%22%2C%22lastName%22%3A%22Barbeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20L.%22%2C%22lastName%22%3A%22Mann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%20G.%22%2C%22lastName%22%3A%22Haygood%22%7D%5D%2C%22abstractNote%22%3A%22Trichodesmium%20colonies%20contain%20an%20abundant%20microbial%20consortium%20that%20is%20likely%20to%20play%20a%20role%20in%20nutrient%20cycling%20within%20the%20colony.%20This%20study%20used%20laboratory%20cultures%20of%20Trichodesmium%20and%20two%20genome-sequenced%20strains%20of%20bacteria%20typical%20of%20Trichodesmium-associated%20microbes%20to%20develop%20an%20understanding%20of%20the%20cycling%20of%20iron%2C%20a%20potentially%20limiting%20micronutrient%2C%20within%20Trichodesmium%20colonies.%20We%20found%20that%20the%20ferric%20siderophores%20desferrioxamine%20B%20and%20aerobactin%20were%20not%20readily%20bioavailable%20to%20Trichodesmium%2C%20relative%20to%20ferric%20chloride%20or%20citrate-associated%20iron.%20In%20contrast%2C%20the%20representative%20bacterial%20strains%20we%20studied%20were%20able%20to%20acquire%20iron%20from%20all%20of%20the%20iron%20sources%2C%20implying%20that%20naturally%20occurring%20Trichodesmium-associated%20bacteria%20may%20be%20capable%20of%20utilizing%20a%20more%20diverse%20array%20of%20iron%20sources%20than%20Trichodesmium.%20From%20the%20organism-specific%20uptake%20data%20collected%20in%20this%20study%2C%20a%20theoretical%20Trichodesmium%20colony%20was%20designed%20to%20model%20whole%20colony%20iron%20uptake.%20The%20bacteria%20accounted%20for%20most%20%28%3E%2070%25%29%20of%20the%20iron%20acquired%20by%20the%20colony%2C%20highlighting%20the%20importance%20of%20determining%20organism-specific%20uptake%20in%20a%20complex%20environment.%20Our%20findings%20suggest%20that%2C%20although%20they%20may%20share%20the%20same%20micro-environment%2C%20Trichodesmium%20and%20its%20colony-associated%20microbial%20cohort%20may%20differ%20substantially%20in%20terms%20of%20iron%20acquisition%20strategy.%22%2C%22date%22%3A%22Jul%202012%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.1111%5C%2Fj.1462-2920.2011.02653.x%22%2C%22ISSN%22%3A%221462-2912%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A14Z%22%7D%7D%2C%7B%22key%22%3A%22DLLS5NEJ%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22King%20et%20al.%22%2C%22parsedDate%22%3A%222012-01%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EKing%2C%20A.%20L.%2C%20Buck%2C%20K.%20N.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282012%29.%20Quasi-Lagrangian%20drifter%20studies%20of%20iron%20speciation%20and%20cycling%20off%20Point%20Conception%2C%20California.%20%3Ci%3EMarine%20Chemistry%3C%5C%2Fi%3E%2C%20%3Ci%3E128%3C%5C%2Fi%3E%2C%201%26%23x2013%3B12.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2011.11.001%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2011.11.001%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Quasi-Lagrangian%20drifter%20studies%20of%20iron%20speciation%20and%20cycling%20off%20Point%20Conception%2C%20California%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20L.%22%2C%22lastName%22%3A%22King%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20N.%22%2C%22lastName%22%3A%22Buck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22The%20distribution%20and%20speciation%20of%20dissolved%20Fe%20%28dFe%29%20were%20measured%20during%20four%20quasi-Lagrangian%20drogued%20drifter%20studies%20%28similar%20to%204%20d%20duration%20each%29%20that%20were%20conducted%20in%20the%20southern%20California%20Current%20System%20in%20May%202006%20and%20April%202007.%20Three%20of%20the%20four%20drifter%20studies%20were%20within%20the%20coastal%20upwelling%20regime%20and%20one%20drifter%20study%20was%20in%20a%20warm-core%20anticyclonic%20eddy.%20Incubation%20bottle%20experiments%20were%20also%20conducted%20to%20determine%20the%20degree%20of%20phytoplankton%20Fe%20limitation%20and%20to%20assess%20changes%20in%20the%20concentration%20of%20Fe-binding%20ligands.%20In%20the%20coastal%20upwelling%20drifter%20studies%2C%20in%20situ%20dFe%20%281.4-1.8%20nM%29%20and%20macronutrients%20were%20initially%20high%20and%20declined%20over%20time.%20Fe%20addition%20incubation%20experiments%20indicated%20that%20the%20phytoplankton%20community%20was%20not%20Fe%20limited%20at%20the%20beginning%20of%20the%20coastal%20upwelling%20drifter%20experiments%20%28when%20mu%20M%20nitrate%3AnM%20dFe%20ratios%20were%20similar%20to%207-8%29.%20By%20the%20end%20of%20two%20of%20the%20three%20drifter%20studies%20%28when%20mu%20M%20nitrate%3AnM%20dFe%20ratios%20were%20similar%20to%2012-19%29%2C%20Fe%20addition%20resulted%20in%20larger%20nitrate%20and%20silicic%20acid%20drawdown%2C%20and%20larger%20accumulations%20in%20chlorophyll%20a%2C%20particulate%20organic%20carbon%20and%20nitrogen%2C%20and%20diatom%20and%20dinoflagellate-specific%20carotenoid%20pigments.%20Fe%20speciation%20was%20measured%20in%20situ%20in%20three%20of%20the%20four%20drifter%20studies%20with%20stronger%20L-1-type%20ligands%20found%20to%20be%20present%20in%20excess%20of%20dFe%20in%20all%20samples.%20In%20Fe%20speciation%20incubation%20experiments.%20L-1-type%20ligand%20production%20was%20observed%20in%20conjunction%20with%20phytoplankton%20growth%20under%20Fe-limiting%20conditions.%20The%20results%20presented%20here%20support%20and%20add%20a%20quasi-Lagrangian%20perspective%20to%20previous%20observations%20of%20dFe%20and%20macronutrient%20cycling%20over%20space%20and%20time%20within%20the%20California%20coastal%20upwelling%20regime%2C%20including%20Fe%20limitation%20within%20the%20phytoplankton%20community%20in%20this%20region%20and%20the%20biological%20production%20of%20Fe-binding%20ligands%20concomitant%20with%20Fe%20limitation.%20%28C%29%202011%20Elsevier%20B.V.%20All%20rights%20reserved.%22%2C%22date%22%3A%22Jan%202012%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.marchem.2011.11.001%22%2C%22ISSN%22%3A%220304-4203%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A17Z%22%7D%7D%2C%7B%22key%22%3A%227J3E8KXX%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hopkinson%20and%20Barbeau%22%2C%22parsedDate%22%3A%222012-01%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHopkinson%2C%20B.%20M.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282012%29.%20Iron%20transporters%20in%20marine%20prokaryotic%20genomes%20and%20metagenomes.%20%3Ci%3EEnvironmental%20Microbiology%3C%5C%2Fi%3E%2C%20%3Ci%3E14%3C%5C%2Fi%3E%281%29%2C%20114%26%23x2013%3B128.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fj.1462-2920.2011.02539.x%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fj.1462-2920.2011.02539.x%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Iron%20transporters%20in%20marine%20prokaryotic%20genomes%20and%20metagenomes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20M.%22%2C%22lastName%22%3A%22Hopkinson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22In%20the%20pelagic%20environment%2C%20iron%20is%20a%20scarce%20but%20essential%20micronutrient.%20The%20iron%20acquisition%20capabilities%20of%20selected%20marine%20bacteria%20have%20been%20investigated%2C%20but%20the%20recent%20proliferation%20of%20marine%20prokaryotic%20genomes%20and%20metagenomes%20offers%20a%20more%20comprehensive%20picture%20of%20microbial%20iron%20uptake%20pathways%20in%20the%20ocean.%20Searching%20these%20data%20sets%2C%20we%20were%20able%20to%20identify%20uptake%20mechanisms%20for%20Fe3%2B%2C%20Fe2%2B%20and%20iron%20chelates%20%28e.g.%20siderophore%20and%20haem%20iron%20complexes%29.%20Transport%20of%20iron%20chelates%20is%20accomplished%20by%20TonB-dependent%20transporters%20%28TBDTs%29.%20After%20clustering%20the%20TBDTs%20from%20marine%20prokaryotic%20genomes%2C%20we%20identified%20TBDT%20clusters%20for%20the%20transport%20of%20hydroxamate%20and%20catecholate%20siderophore%20iron%20complexes%20and%20haem%20using%20gene%20neighbourhood%20analysis%20and%20co-clustering%20of%20TBDTs%20of%20known%20function.%20The%20genomes%20also%20contained%20two%20classes%20of%20siderophore%20biosynthesis%20genes%3A%20NRPS%20%28non-ribosomal%20peptide%20synthase%29%20genes%20and%20NIS%20%28NRPS%20Independent%20Siderophore%29%20genes.%20The%20most%20common%20iron%20transporters%2C%20in%20both%20the%20genomes%20and%20metagenomes%2C%20were%20Fe3%2B%20ABC%20transporters.%20Iron%20uptake-related%20TBDTs%20and%20siderophore%20biosynthesis%20genes%20were%20less%20common%20in%20pelagic%20marine%20metagenomes%20relative%20to%20the%20genomic%20data%20set%2C%20in%20part%20because%20Pelagibacter%20ubique%20and%20Prochlorococcus%20species%2C%20which%20almost%20entirely%20lacked%20these%20Fe%20uptake%20systems%2C%20dominate%20the%20metagenomes.%20Our%20results%20are%20largely%20consistent%20with%20current%20knowledge%20of%20iron%20speciation%20in%20the%20ocean%2C%20but%20suggest%20that%20in%20certain%20niches%20the%20ability%20to%20acquire%20siderophores%20and%5C%2For%20haem%20iron%20chelates%20is%20beneficial.%22%2C%22date%22%3A%22Jan%202012%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.1111%5C%2Fj.1462-2920.2011.02539.x%22%2C%22ISSN%22%3A%221462-2912%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A15Z%22%7D%7D%2C%7B%22key%22%3A%22BEKC2QJ5%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22King%20and%20Barbeau%22%2C%22parsedDate%22%3A%222011-03%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EKing%2C%20A.%20L.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282011%29.%20Dissolved%20iron%20and%20macronutrient%20distributions%20in%20the%20southern%20California%20Current%20System.%20%3Ci%3EJournal%20of%20Geophysical%20Research-Oceans%3C%5C%2Fi%3E%2C%20%3Ci%3E116%3C%5C%2Fi%3E.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1029%5C%2F2010jc006324%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1029%5C%2F2010jc006324%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Dissolved%20iron%20and%20macronutrient%20distributions%20in%20the%20southern%20California%20Current%20System%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20L.%22%2C%22lastName%22%3A%22King%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22The%20distribution%20of%20dissolved%20iron%20in%20the%20southern%20California%20Current%20System%20%28sCCS%29%20is%20presented%20from%20seven%20research%20cruises%20between%202002%20and%202006.%20Dissolved%20iron%20concentrations%20were%20generally%20low%20in%20most%20of%20the%20study%20area%20%28%3C0.5%20nM%29%2C%20although%20high%20mixed%20layer%20and%20water%20column%20dissolved%20iron%20concentrations%20%28up%20to%208%20nM%29%20were%20found%20to%20be%20associated%20with%20coastal%20upwelling%2C%20both%20along%20the%20continental%20margin%20and%20some%20island%20platforms.%20A%20significant%20supply%20of%20iron%20was%20probably%20not%20from%20a%20deep%20remineralized%20source%20but%20rather%20from%20the%20continental%20shelf%20and%20bottom%20boundary%20layer%20as%20identified%20in%20previous%20studies%20along%20the%20central%20and%20northern%20California%20coast.%20With%20distance%20offshore%2C%20dissolved%20iron%20decreased%20more%20rapidly%20relative%20to%20nitrate%20in%20a%20transition%20zone%2010-250%20km%20offshore%20during%20spring%20and%20summer%2C%20resulting%20in%20relatively%20high%20ratios%20of%20nitrate%3A%20dissolved%20iron.%20Higher%20nitrate%3A%20dissolved%20iron%20ratios%20could%20be%20the%20result%20of%20utilization%20and%20scavenging%20in%20addition%20to%20an%20overall%20lower%20supply%20of%20iron%20relative%20to%20nitrate%20in%20the%20offshore%20transition%20zones.%20The%20low%20supply%20of%20iron%20leads%20to%20phytoplankton%20iron%20limitation%20and%20a%20depletion%20in%20silicic%20acid%20relative%20to%20nitrate%20in%20the%20coastal%20upwelling%20and%20transition%20zones%20of%20the%20sCCS.%22%2C%22date%22%3A%22Mar%202011%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.1029%5C%2F2010jc006324%22%2C%22ISSN%22%3A%220148-0227%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A14Z%22%7D%7D%2C%7B%22key%22%3A%22YI55DGGR%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Buck%20et%20al.%22%2C%22parsedDate%22%3A%222010-10%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBuck%2C%20K.%20N.%2C%20Selph%2C%20K.%20E.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282010%29.%20Iron-binding%20ligand%20production%20and%20copper%20speciation%20in%20an%20incubation%20experiment%20of%20Antarctic%20Peninsula%20shelf%20waters%20from%20the%20Bransfield%20Strait%2C%20Southern%20Ocean.%20%3Ci%3EMarine%20Chemistry%3C%5C%2Fi%3E%2C%20%3Ci%3E122%3C%5C%2Fi%3E%281%26%23x2013%3B4%29%2C%20148%26%23x2013%3B159.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2010.06.002%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.marchem.2010.06.002%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Iron-binding%20ligand%20production%20and%20copper%20speciation%20in%20an%20incubation%20experiment%20of%20Antarctic%20Peninsula%20shelf%20waters%20from%20the%20Bransfield%20Strait%2C%20Southern%20Ocean%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20N.%22%2C%22lastName%22%3A%22Buck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20E.%22%2C%22lastName%22%3A%22Selph%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22The%20evolution%20of%20dissolved%20iron%20%28Fe%29%20and%20copper%20%28Cu%29%20speciation%20was%20followed%20through%20a%20simulated%20spring%20bloom%20event%20in%20a%2015-day%20incubation%20experiment%20of%20natural%20seawater%20collected%20during%20austral%20winter%20from%20high%20macronutrient%20high%20Fe%20waters%20of%20Bransfield%20Strait%20in%20the%20Southern%20Ocean.%20The%20incubation%20experiment%20included%20unamended%20bottles%20as%20well%20as%20Fe%20additions%20using%20the%20stable%20isotope%20of%20Fe%2C%20Fe-57.%20as%20inorganic%20%28%28FeCl3%29-Fe-57%29%20and%20organic%20%28Fe-57-aerobactin%2C%20Fe-57-desferrioxamine%20B%29%20amendments.%20Exposure%20to%20summer%20light%20conditions%20resulted%20in%20substantial%20growth%20for%20all%20treatments%2C%20mimicking%20the%20initiation%20of%20a%20spring%20bloom.%20The%20addition%20of%20Fe%20resulted%20in%20a%2030%25%20increase%20in%20phytoplankton%20biomass%20over%20unamended%20controls%20by%20day%2015%2C%20indicating%20that%20the%20unamended%20waters%20became%20Fe%20limited%20despite%20initially%20elevated%20dissolved%20Fe%20concentrations.%20Dissolved%20Cu%20and%20Cu%20speciation%20remained%20largely%20unchanged%20for%20all%20treatments%20of%20the%20incubation%2C%20with%20Cu%20speciation%20dominated%20by%20exceedingly%20strong%20Cu-binding%20ligands%20%28log%20K-CuL1.Cu2%2B%28Cond%29%20similar%20to%2016%29%20and%20low%20resultant%20Cu2%2B%20concentrations%20%2810%28-16.3%20%2B%5C%2F-%200.3%29%20mol%20L-1%29.%20In%20only%20the%20unamended%20light%20bottles%2C%20strong%20Fe-binding%20ligands%20were%20produced%20over%20the%20course%20of%20the%20experiment.%20The%20observed%20production%20of%20strong%20Fe-binding%20ligands%20in%20the%20control%20bottles%20that%20became%20Fe-limited%20supports%20the%20important%20role%20of%20biologically%20produced%20siderophore-type%20natural%20ligands%20in%20the%20marine%20Fe%20cycle.%20%28C%29%202010%20Elsevier%20B.V.%20All%20rights%20reserved.%22%2C%22date%22%3A%22Oct%202010%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.marchem.2010.06.002%22%2C%22ISSN%22%3A%220304-4203%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A15Z%22%7D%7D%2C%7B%22key%22%3A%22DBXYB8YE%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Dupont%20et%20al.%22%2C%22parsedDate%22%3A%222010-04%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EDupont%2C%20C.%20L.%2C%20Buck%2C%20K.%20N.%2C%20Palenik%2C%20B.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20%282010%29.%20Nickel%20utilization%20in%20phytoplankton%20assemblages%20from%20contrasting%20oceanic%20regimes.%20%3Ci%3EDeep-Sea%20Research%20Part%20I-Oceanographic%20Research%20Papers%3C%5C%2Fi%3E%2C%20%3Ci%3E57%3C%5C%2Fi%3E%284%29%2C%20553%26%23x2013%3B566.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.dsr.2009.12.014%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.dsr.2009.12.014%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Nickel%20utilization%20in%20phytoplankton%20assemblages%20from%20contrasting%20oceanic%20regimes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christopher%20L.%22%2C%22lastName%22%3A%22Dupont%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kristen%20N.%22%2C%22lastName%22%3A%22Buck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brian%22%2C%22lastName%22%3A%22Palenik%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katherine%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22In%20most%20oceanic%20environments%2C%20dissolved%20nickel%20%28Ni%29%20concentrations%20are%20drawn%20clown%20in%20surface%20waters%20with%20increasing%20concentrations%20at%20depth%2C%20implying%20a%20role%20for%20biology%20in%20the%20geochemical%20distribution%20of%20Ni%20Studies%20with%20phytoplankton%20isolates%20from%20the%20surface%20ocean%20have%20established%20the%20biochemical%20roles%20of%20Ni%20in%20the%20assimilation%20of%20urea%20and%20oxidative%20defense%20To%20determine%20if%20these%20requirements%20are%20relevant%20in%20natural%20marine%20planktonic%20assemblages%2C%20bottle-based%20fertilization%20experiments%20were%20used%20to%20test%20the%20effects%20of%20low-level%20additions%20of%20Ni.%20urea%2C%20or%20both%20Ni%20and%20urea%20to%20surface%20waters%20at%20several%20locations%20offshore%20of%20Peru%20and%20California%2C%20as%20well%20as%20in%20the%20Gulf%20of%20California%20Urea%20and%20Ni%28%2B%29%20urea%20additions%20consistently%20promoted%20phytoplankton%20growth%20relative%20to%20control%20and%20%2BNi%20treatments%2C%20except%20in%20a%20coastal%20upwelling%20site%20and%20Peruvian%20water.%20No%20effect%20was%20observed%20in%20the%20upwelling%20site%2C%20but%20in%20Peruvian%20waters%20urea%20additions%20resulted%20in%20increased%20phytoplankton%20pigments%20and%20phosphate%20drawdown%20only%20when%20Ni%20was%20added%20concurrently%2C%20suggesting%20a%20biochemically%20dependent%20Ni-urea%20colimitation%20In%20the%20Gulf%20of%20California%2C%20Ni%20additions%20without%20urea%20resulted%20in%20increased%20abundances%20of%20cyanobacteria%2C%20picoeukaryotes%2C%20and%20the%20corresponding%20pigments%20As%20urea%20additions%20showed%20the%20overall%20phytoplankton%20community%20was%20also%20urea-limited%2C%20it%20appears%20that%20the%20cyanobactena%20and%20potentially%20the%20picoeukaryotes%20were%20colimited%20by%20Ni%20and%20urea%20in%20a%20biochemically%20independent%20fashion.%20In%20parallel%2C%20radiotracer-based%20uptake%20experiments%20were%20used%20to%20study%20the%20kinetics%20and%20spatial%20variation%20of%20biological%20Ni%20assimilation.%20In%20these%20experiments%2C%20the%20added%20radiotracer%20rarely%20equilibrated%20with%20the%20natural%20Ni%20present%2C%20precluding%20estimates%20a%20determination%20of%20in%20situ%20Ni%20uptake%20rates%20and%20suggesting%20that%20much%20of%20the%20natural%20Ni%20was%20not%20bioavailable.%20The%20lack%20of%20equilibration%20likely%20did%20not%20preclude%20the%20measurement%20of%20community%20Ni%20uptake%20kinetics%2C%20nor%20the%20comparison%20of%20measured%20rates%20between%20locations%20The%20highest%20V%28max%29K%28p%29%28-1%29%20values%2C%20which%20reflect%20a%20competitive%20advantage%20in%20Ni%20acquisition%20at%20low%20concentrations%2C%20were%20observed%20in%20stratified%20nitrogen-deplete%20communities%2C%20potentially%20linking%20Ni%20and%20nitrogen%20biogeochemistry%20in%20a%20manner%20consistent%20with%20the%20biochemical%20utilization%20of%20Ni.%20Overall%2C%20uptake%20rates%20were%20higher%20in%20the%20euphotic%20rather%20than%20non-euphotic%20zone%20communities%2C%20directly%20reconciling%20the%20nutrient-like%20depth%20profile%20of%20Ni%20The%20Ni%20uptake%20rates%20observed%20at%20the%20nitrate-replete%20Fe-deplete%20Peru%20stations%20were%20an%20order%20of%20magnitude%20lower%20than%20the%20other%20sites%20This%20result%20agrees%20with%20calculations%20suggesting%20that%20saturation%20of%20the%20cell%20surface%20with%20Ni%20and%20iron%20%28Fe%29%20transporters%20may%20limit%20uptake%20rates%20in%20low%20Fe%20waters.%20%28C%29%202010%20Elsevier%20Ltd.%20All%20rights%20reserved%22%2C%22date%22%3A%22Apr%202010%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.dsr.2009.12.014%22%2C%22ISSN%22%3A%220967-0637%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A16Z%22%7D%7D%2C%7B%22key%22%3A%22C6SK2T6D%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hopkinson%20et%20al.%22%2C%22parsedDate%22%3A%222008-10%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHopkinson%2C%20B.%20M.%2C%20Roe%2C%20K.%20L.%2C%20%26amp%3B%20%3Cstrong%3EBarbeau%3C%5C%2Fstrong%3E%2C%20K.%20A.%20%282008%29.%20Heme%20uptake%20by%20Microscilla%20marina%20and%20evidence%20for%20heme%20uptake%20systems%20in%20the%20genomes%20of%20diverse%20marine%20bacteria.%20%3Ci%3EApplied%20and%20Environmental%20Microbiology%3C%5C%2Fi%3E%2C%20%3Ci%3E74%3C%5C%2Fi%3E%2820%29%2C%206263%26%23x2013%3B6270.%20%3Ca%20class%3D%27zp-DOIURL%27%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Faem.00964-08%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Faem.00964-08%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Heme%20uptake%20by%20Microscilla%20marina%20and%20evidence%20for%20heme%20uptake%20systems%20in%20the%20genomes%20of%20diverse%20marine%20bacteria%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20M.%22%2C%22lastName%22%3A%22Hopkinson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20L.%22%2C%22lastName%22%3A%22Roe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20A.%22%2C%22lastName%22%3A%22Barbeau%22%7D%5D%2C%22abstractNote%22%3A%22The%20ability%20to%20acquire%20diverse%20and%20abundant%20forms%20of%20iron%20would%20be%20expected%20to%20confer%20a%20survival%20advantage%20in%20the%20marine%20environment%2C%20where%20iron%20is%20scarce.%20Marine%20bacteria%20are%20known%20to%20use%20siderophores%20and%20inorganic%20iron%2C%20but%20their%20ability%20to%20use%20heme%2C%20an%20abundant%20intracellular%20iron%20form%2C%20has%20only%20been%20examined%20preliminarily.%20Microscilla%20marina%2C%20a%20cultured%20relative%20of%20a%20bacterial%20group%20frequently%20found%20on%20marine%20particulates%2C%20was%20used%20as%20a%20model%20organism%20to%20examine%20heme%20uptake.%20Searches%20of%20the%20genome%20revealed%20analogs%20to%20known%20heme%20transport%20proteins%2C%20and%20reverse%20transcription-quantitative%20PCR%20analysis%20of%20these%20genes%20showed%20that%20they%20were%20expressed%20and%20upregulated%20under%20iron%20stress%20and%20during%20growth%20on%20heme.%20M.%20marina%20was%20found%20to%20take%20up%20heme-bound%20iron%20and%20could%20grow%20on%20heme%20as%20a%20sole%20iron%20source%2C%20supporting%20the%20genetic%20evidence%20for%20heme%20transport.%20Similar%20putative%20heme%20transport%20components%20were%20identified%20in%20the%20genomes%20of%20diverse%20marine%20bacteria.%20These%20systems%20were%20found%20in%20the%20genomes%20of%20many%20bacteria%20thought%20to%20be%20particle%20associated%20but%20were%20lacking%20in%20known%20free-living%20organisms%20%28e.g.%2C%20Pelagibacter%20ubique%20and%20marine%20cyanobacteria%29.%20This%20distribution%20of%20transporters%20is%20consistent%20with%20the%20hydrophobic%2C%20light-sensitive%20nature%20of%20heme%2C%20suggesting%20that%20it%20is%20primarily%20available%20on%20phytoplankton%20or%20detritus%20or%20in%20nutrient-rich%20environments.%22%2C%22date%22%3A%22Oct%202008%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.1128%5C%2Faem.00964-08%22%2C%22ISSN%22%3A%220099-2240%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MWYMG4GN%22%5D%2C%22dateModified%22%3A%222022-05-24T23%3A28%3A15Z%22%7D%7D%5D%7D
Manck, L. E., Coale, T. H., Stephens, B. M., Forsch, K. O., Aluwihare, L. I., Dupont, C. L., Allen, A. E., & Barbeau, K. A. (2024). Iron limitation of heterotrophic bacteria in the California Current System tracks relative availability of organic carbon and iron. The ISME Journal, 18(1), wrae061. https://doi.org/10.1093/ismejo/wrae061
Lampe, R. H., Coale, T. H., Forsch, K. O., Jabre, L. J., Kekuewa, S., Bertrand, E. M., Horák, A., Oborník, M., Rabines, A. J., Rowland, E., Zheng, H., Andersson, A. J., Barbeau, K. A., & Allen, A. E. (2023). Short-term acidification promotes diverse iron acquisition and conservation mechanisms in upwelling-associated phytoplankton. Nature Communications, 14(1), 7215. https://doi.org/10.1038/s41467-023-42949-1
Forsch, K. O., Fulton, K. C., Weiss, M. M., Krause, J. W., Stukel, M. R., & Barbeau, K. A. (2023). Iron Limitation and Biogeochemical Effects in Southern California Current Coastal Upwelling Filaments. Journal of Geophysical Research: Oceans, 128(11), e2023JC019961. https://doi.org/10.1029/2023JC019961
Forsch, K. O., Hahn-Woernle, L., Sherrell, R. M., Roccanova, V. J., Bu, K. X., Burdige, D., Vernet, M., & Barbeau, K. A. (2021). Seasonal dispersal of fjord meltwaters as an important source of iron and manganese to coastal Antarctic phytoplankton. Biogeosciences, 18(23), 6349–6375. https://doi.org/10.5194/bg-18-6349-2021
Moore, L. E., Heller, M. I., Barbeau, K. A., Moffett, J. W., & Bundy, R. M. (2021). Organic complexation of iron by strong ligands and siderophores in the eastern tropical North Pacific oxygen deficient zone. Marine Chemistry, 236, 16. https://doi.org/10.1016/j.marchem.2021.104021
Manck, L. E., Park, J., Tully, B. J., Poire, A. M., Bundy, R. M., Dupont, C. L., & Barbeau, K. A. (2021). Petrobactin, a siderophore produced by Alteromonas, mediates community iron acquisition in the global ocean. Isme Journal, 12. https://doi.org/10.1038/s41396-021-01065-y
Ruacho, A., Bundy, R. M., Till, C. P., Roshan, S., Wu, J. F., & Barbeau, K. A. (2020). Organic dissolved copper speciation across the US GEOTRACES equatorial Pacific zonal transect GP16. Marine Chemistry, 225. https://doi.org/10.1016/j.marchem.2020.103841
Pan, B. J., Vernet, M., Manck, L., Forsch, K., Ekern, L., Mascioni, M., Barbeau, K. A., Almandoz, G. O., & Orona, A. J. (2020). Environmental drivers of phytoplankton taxonomic composition in an Antarctic fjord. Progress in Oceanography, 183. https://doi.org/10.1016/j.pocean.2020.102295
Manck, L. E., Espinoza, J. L., Dupont, C. L., & Barbeau, K. A. (2020). Transcriptomic study of substrate-specific transport mechanisms for iron and carbon in the marine copiotroph Alteromonas macleodii. MSystems, 5(2). https://doi.org/10.1128/mSystems.00070-20
Stukel, M. R., & Barbeau, K. A. (2020). Investigating the nutrient landscape in a coastal upwelling region and its relationship to the biological carbon pump. Geophysical Research Letters, 47(6). https://doi.org/10.1029/2020gl087351
Coale, T. H., Moosburner, M., Horak, A., Obornik, M., Barbeau, K. A., & Allen, A. E. (2019). Reduction-dependent siderophore assimilation in a model pennate diatom. Proceedings of the National Academy of Sciences of the United States of America, 116(47), 23609–23617. https://doi.org/10.1073/pnas.1907234116
Meskhidze, N., Volker, C., Al-Abadleh, H. A., Barbeau, K., Bressac, M., Buck, C., Bundy, R. M., Croot, P., Feng, Y., Ito, A., Johansen, A. M., Landing, W. M., Mao, J. Q., Myriokefalitakis, S., Ohnemus, D., Pasquier, B., & Ye, Y. (2019). Perspective on identifying and characterizing the processes controlling iron speciation and residence time at the atmosphere-ocean interface. Marine Chemistry, 217. https://doi.org/10.1016/j.marchem.2019.103704
Jiang, M., Measures, C. I., Barbeau, K. A., Charette, M. A., Gille, S. T., Hatta, M., Kahru, M., Mitchell, B. G., Garabato, A. C. N., Reiss, C., Selph, K., & Zhou, M. (2019). Fe sources and transport from the Antarctic Peninsula shelf to the southern Scotia Sea. Deep-Sea Research Part I-Oceanographic Research Papers, 150. https://doi.org/10.1016/j.dsr.2019.06.006
Stukel, M. R., Kelly, T. B., Aluwihare, L. I., Barbeau, K. A., Goericke, R., Krause, J. W., Landry, M. R., & Ohman, M. D. (2019). The Carbon:(234)Thorium ratios of sinking particles in the California current ecosystem 1: relationships with plankton ecosystem dynamics. Marine Chemistry, 212, 1–15. https://doi.org/10.1016/j.marchem.2019.01.003
Chappell, P. D., Armbrust, E. V., Barbeau, K. A., Bundy, R. M., Moffett, J. W., Vedamati, J., & Jenkins, B. D. (2019). Patterns of diatom diversity correlate with dissolved trace metal concentrations and longitudinal position in the northeast Pacific coastal-offshore transition zone. Marine Ecology Progress Series, 609, 69–86. https://doi.org/10.3354/meps12810
Hogle, S. L., Dupont, C. L., Hopkinson, B. M., King, A. L., Buck, K. N., Roe, K. L., Stuart, R. K., Allen, A. E., Mann, E. L., Johnson, Z. I., & Barbeau, K. A. (2018). Pervasive iron limitation at subsurface chlorophyll maxima of the California Current. Proceedings of the National Academy of Sciences of the United States of America, 115(52), 13300–13305. https://doi.org/10.1073/pnas.1813192115
McQuaid, J. B., Kustka, A. B., Oborník, M., Horák, A., McCrow, J. P., Karas, B. J., Zheng, H., Kindeberg, T., Andersson, A. J., Barbeau, K. A., & Allen, A. E. (2018). Carbonate-sensitive phytotransferrin controls high-affinity iron uptake in diatoms. Nature, 555, 534. https://doi.org/10.1038/nature25982
Stuart, R. K., Bundy, R., Buck, K., Ghassemain, M., Barbeau, K., & Palenik, B. (2017). Copper toxicity response influences mesotrophic Synechococcus community structure. Environmental Microbiology, 19(2), 756–769. https://doi.org/10.1111/1462-2920.13630
Stukel, M. R., Aluwihare, L. I., Barbeau, K. A., Chekalyuk, A. M., Goericke, R., Miller, A. J., Ohman, M. D., Ruacho, A., Song, H., Stephens, B. M., & Landry, M. R. (2017). Mesoscale ocean fronts enhance carbon export due to gravitational sinking and subduction. Proceedings of the National Academy of Sciences of the United States of America, 114(6), 1252–1257. https://doi.org/10.1073/pnas.1609435114
Hogle, S. L., Brahamsha, B., & Barbeau, K. A. (2017). Direct heme uptake by phytoplankton-associated Roseobacter bacteria. MSystems, 2. https://doi.org/10.1128/mSystems.00124-16
Boiteau, R. M., Till, C. P., Ruacho, A., Bundy, R. M., Hawco, N. J., McKenna, A. M., Barbeau, K. A., Bruland, K. W., Saito, M. A., & Repeta, D. J. (2016). Structural characterization of natural nickel and copper binding ligands along the US GEOTRACES Eastern Pacific Zonal Transect. Frontiers in Marine Science, 3(243). https://doi.org/10.3389/fmars.2016.00243
Hogle, S. L., Bundy, R. M., Blanton, J. M., Allen, E. E., & Barbeau, K. A. (2016). Copiotrophic marine bacteria are associated with strong iron-binding ligand production during phytoplankton blooms. Limnology and Oceanography Letters. https://doi.org/10.1002/lol2.10026
Semeniuk, D. M., Bundy, R. M., Posacka, A. M., Robert, M., Barbeau, K. A., & Maldonado, M. T. (2016). Using 67Cu to study the biogeochemical cycling of copper in the northeast subarctic Pacific Ocean. Frontiers in Marine Science, 3(78). https://doi.org/10.3389/fmars.2016.00078
Hogle, S. L., Thrash, J. C., Dupont, C. L., & Barbeau, K. A. (2016). Trace metal acquisition by marine heterotrophic bacterioplankton with contrasting trophic strategies. Applied and Environmental Microbiology, 82(5), 1613–1624. https://doi.org/10.1128/aem.03128-15
Bundy, R. M., Jiang, M., Carter, M., & Barbeau, K. A. (2016). Iron-binding ligands in the southern California Current System: Mechanistic studies. Frontiers in Marine Science, 3(27). https://doi.org/10.3389/fmars.2016.00027
Semeniuk, D. M., Taylor, R. L., Bundy, R. M., Johnson, W. K., Cullen, J. T., Robert, M., Barbeau, K. A., & Maldonado, M. T. (2016). Iron-copper interactions in iron-limited phytoplankton in the northeast subarctic Pacific Ocean. Limnology and Oceanography, 61(1), 279–297. https://doi.org/10.1002/lno.10210
Brzezinski, M. A., Krause, J. W., Bundy, R. M., Barbeau, K. A., Franks, P., Goericke, R., Landry, M. R., & Stukel, M. R. (2015). Enhanced silica ballasting from iron stress sustains carbon export in a frontal zone within the California Current. Journal of Geophysical Research-Oceans, 120(7), 4654–4669. https://doi.org/10.1002/2015jc010829
Bundy, R. M., Abdulla, H. A. N., Hatcher, P. G., Biller, D. V., Buck, K. N., & Barbeau, K. A. (2015). Iron-binding ligands and humic substances in the San Francisco Bay estuary and estuarine-influenced shelf regions of coastal California. Marine Chemistry, 173, 183–194. https://doi.org/10.1016/j.marchem.2014.11.005
Pizeta, I., Sander, S. G., Hudson, R. J. M., Omanovic, D., Baars, O., Barbeau, K. A., Buck, K. N., Bundy, R. M., Carrasco, G., Croot, P. L., Garnier, C., Gerringa, L. J. A., Gledhill, M., Hirose, K., Kondo, Y., Laglera, L. M., Nuester, J., Rijkenberg, M. J. A., Takeda, S., … Wells, M. (2015). Interpretation of complexometric titration data: An intercomparison of methods for estimating models of trace metal complexation by natural organic ligands. Marine Chemistry, 173, 3–24. https://doi.org/10.1016/j.marchem.2015.03.006
Fitzsimmons, J. N., Bundy, R. M., Al-Subiai, S. N., Barbeau, K. A., & Boyle, E. A. (2015). The composition of dissolved iron in the dusty surface ocean: An exploration using size-fractionated iron-binding ligands. Marine Chemistry, 173, 125–135. https://doi.org/10.1016/j.marchem.2014.09.002
Semeniuk, D. M., Bundy, R. M., Payne, C. D., Barbeau, K. A., & Maldonado, M. T. (2015). Acquisition of organically complexed copper by marine phytoplankton and bacteria in the northeast subarctic Pacific Ocean. Marine Chemistry, 173, 222–233. https://doi.org/10.1016/j.marchem.2015.01.005
Dupont, C. L., McCrow, J. P., Valas, R., Moustafa, A., Walworth, N., Goodenough, U., Roth, R., Hogle, S. L., Bai, J., Johnson, Z. I., Mann, E., Palenik, B., Barbeau, K. A., Craig Venter, J., & Allen, A. E. (2014). Genomes and gene expression across light and productivity gradients in eastern subtropical Pacific microbial communities. Isme Journal, 9(5), 1076–1092. https://doi.org/10.1038/ismej.2014.198
Roe, K. L., & Barbeau, K. A. (2014). Uptake mechanisms for inorganic iron and ferric citrate in Trichodesmium erythraeum IMS101. Metallomics, 6(11), 2042–2051. https://doi.org/10.1039/c4mt00026a
Bundy, R. M., Biller, D. V., Buck, K. N., Bruland, K. W., & Barbeau, K. A. (2014). Distinct pools of dissolved iron-binding ligands in the surface and benthic boundary layer of the California Current. Limnology and Oceanography, 59(3), 769–787. https://doi.org/10.4319/lo.2014.59.3.0769
Hogle, S. L., Barbeau, K. A., & Gledhill, M. (2014). Heme in the marine environment: from cells to the iron cycle. Metallomics, 6(6), 1107–1120. https://doi.org/10.1039/c4mt00031e
Earley, P. J., Swope, B. L., Barbeau, K., Bundy, R., McDonald, J. A., & Rivera-Duarte, I. (2014). Life cycle contributions of copper from vessel painting and maintenance activities. Biofouling, 30(1), 51–68. https://doi.org/10.1080/08927014.2013.841891
Roe, K. L., Hogle, S. L., & Barbeau, K. A. (2013). Utilization of heme as an iron source by marine alphaproteobacteria in the roseobacter clade. Applied and Environmental Microbiology, 79(18), 5753–5762. https://doi.org/10.1128/aem.01562-13
Ohman, M. D., Barbeau, K., Franks, P. J. S., Goericke, R., Landry, M. R., & Miller, A. J. (2013). Ecological transitions in a coastal upwelling ecosystem. Oceanography, 26(3), 210–219.
Bundy, R. M., Barbeau, K. A., & Buck, K. N. (2013). Sources of strong copper-binding ligands in Antarctic Peninsula surface waters. Deep-Sea Research Part Ii-Topical Studies in Oceanography, 90, 134–146. https://doi.org/10.1016/j.dsr2.2012.07.023
Jiang, M. S., Barbeau, K. A., Selph, K. E., Measures, C. I., Buck, K. N., Azam, F., Mitchell, B. G., & Zhou, M. (2013). The role of organic ligands in iron cycling and primary productivity in the Antarctic Peninsula: A modeling study. Deep-Sea Research Part Ii-Topical Studies in Oceanography, 90, 112–133. https://doi.org/10.1016/j.dsr2.2013.01.029
Hopkinson, B. M., Seegers, B., Hatta, M., Measures, C. I., Mitchell, B. G., & Barbeau, K. A. (2013). Planktonic C:Fe ratios and carrying capacity in the southern Drake Passage. Deep-Sea Research Part Ii-Topical Studies in Oceanography, 90, 102–111. https://doi.org/10.1016/j.dsr2.2012.09.001
Landry, M. R., Ohman, M. D., Goericke, R., Stukel, M. R., Barbeau, K. A., Bundy, R., & Kahru, M. (2012). Pelagic community responses to a deep-water front in the California Current Ecosystem: overview of the A-Front Study. Journal of Plankton Research, 34(9), 739–748. https://doi.org/10.1093/plankt/fbs025
Buck, K. N., Moffett, J., Barbeau, K. A., Bundy, R. M., Kondo, Y., & Wu, J. F. (2012). The organic complexation of iron and copper: an intercomparison of competitive ligand exchange-adsorptive cathodic stripping voltammetry (CLE-ACSV) techniques. Limnology and Oceanography-Methods, 10, 496–515. https://doi.org/10.4319/lom.2012.10.496
Roe, K. L., Barbeau, K., Mann, E. L., & Haygood, M. G. (2012). Acquisition of iron by Trichodesmium and associated bacteria in culture. Environmental Microbiology, 14(7), 1681–1695. https://doi.org/10.1111/j.1462-2920.2011.02653.x
King, A. L., Buck, K. N., & Barbeau, K. A. (2012). Quasi-Lagrangian drifter studies of iron speciation and cycling off Point Conception, California. Marine Chemistry, 128, 1–12. https://doi.org/10.1016/j.marchem.2011.11.001
Hopkinson, B. M., & Barbeau, K. A. (2012). Iron transporters in marine prokaryotic genomes and metagenomes. Environmental Microbiology, 14(1), 114–128. https://doi.org/10.1111/j.1462-2920.2011.02539.x
King, A. L., & Barbeau, K. A. (2011). Dissolved iron and macronutrient distributions in the southern California Current System. Journal of Geophysical Research-Oceans, 116. https://doi.org/10.1029/2010jc006324
Buck, K. N., Selph, K. E., & Barbeau, K. A. (2010). Iron-binding ligand production and copper speciation in an incubation experiment of Antarctic Peninsula shelf waters from the Bransfield Strait, Southern Ocean. Marine Chemistry, 122(1–4), 148–159. https://doi.org/10.1016/j.marchem.2010.06.002
Dupont, C. L., Buck, K. N., Palenik, B., & Barbeau, K. (2010). Nickel utilization in phytoplankton assemblages from contrasting oceanic regimes. Deep-Sea Research Part I-Oceanographic Research Papers, 57(4), 553–566. https://doi.org/10.1016/j.dsr.2009.12.014
Hopkinson, B. M., Roe, K. L., & Barbeau, K. A. (2008). Heme uptake by Microscilla marina and evidence for heme uptake systems in the genomes of diverse marine bacteria. Applied and Environmental Microbiology, 74(20), 6263–6270. https://doi.org/10.1128/aem.00964-08